Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0427 and Shewana3_0428 are separated by 149 nucleotides Shewana3_0428 and Shewana3_0429 are separated by 140 nucleotides
Shewana3_0427: Shewana3_0427 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (RefSeq), at 487,935 to 489,941
_0427
Shewana3_0428: Shewana3_0428 - dihydrolipoamide dehydrogenase (RefSeq), at 490,091 to 491,518
_0428
Shewana3_0429: Shewana3_0429 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (RefSeq), at 491,659 to 496,122
_0429
Position (kb) 490
491
492 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 489.196 kb on - strand, within Shewana3_0427 at 489.299 kb on - strand, within Shewana3_0427 at 489.307 kb on + strand, within Shewana3_0427 at 489.363 kb on - strand, within Shewana3_0427 at 489.472 kb on - strand at 489.486 kb on + strand, within Shewana3_0427 at 489.546 kb on - strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.570 kb on - strand, within Shewana3_0427 at 489.659 kb on + strand, within Shewana3_0427 at 489.675 kb on + strand, within Shewana3_0427 at 491.811 kb on - strand at 491.896 kb on + strand at 491.904 kb on - strand at 491.904 kb on - strand at 492.015 kb on + strand at 492.301 kb on - strand, within Shewana3_0429 at 492.376 kb on - strand, within Shewana3_0429 at 492.423 kb on - strand, within Shewana3_0429 at 492.435 kb on + strand, within Shewana3_0429
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 489,196 - Shewana3_0427 0.63 +1.4 489,299 - Shewana3_0427 0.68 +1.3 489,307 + Shewana3_0427 0.68 +2.6 489,363 - Shewana3_0427 0.71 +1.2 489,472 - +1.9 489,486 + Shewana3_0427 0.77 +1.8 489,546 - Shewana3_0427 0.80 +2.4 489,562 + Shewana3_0427 0.81 +2.3 489,562 + Shewana3_0427 0.81 +2.0 489,562 + Shewana3_0427 0.81 +2.3 489,570 - Shewana3_0427 0.81 +1.0 489,659 + Shewana3_0427 0.86 +2.6 489,675 + Shewana3_0427 0.87 +2.1 491,811 - -2.9 491,896 + -1.7 491,904 - +0.9 491,904 - +0.7 492,015 + -1.9 492,301 - Shewana3_0429 0.14 -1.3 492,376 - Shewana3_0429 0.16 +0.3 492,423 - Shewana3_0429 0.17 +0.5 492,435 + Shewana3_0429 0.17 -0.6
Or see this region's nucleotide sequence