Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0423 and Shewana3_0424 are separated by 184 nucleotides Shewana3_0424 and pdhR are separated by 325 nucleotides
Shewana3_0423: Shewana3_0423 - N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq), at 482,516 to 483,061
_0423
Shewana3_0424: Shewana3_0424 - regulatory protein AmpE (RefSeq), at 483,246 to 484,097
_0424
Shewana3_0425: pdhR - transcriptional regulator PdhR (RefSeq), at 484,423 to 485,175
pdhR
Position (kb) 483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2 at 482.438 kb on + strand at 482.474 kb on - strand at 482.518 kb on - strand at 482.539 kb on - strand at 482.624 kb on - strand, within Shewana3_0423 at 482.637 kb on + strand, within Shewana3_0423 at 482.637 kb on + strand, within Shewana3_0423 at 482.647 kb on + strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.718 kb on + strand, within Shewana3_0423 at 482.762 kb on + strand, within Shewana3_0423 at 482.812 kb on - strand, within Shewana3_0423 at 482.951 kb on - strand, within Shewana3_0423 at 482.963 kb on - strand, within Shewana3_0423 at 482.990 kb on - strand, within Shewana3_0423 at 483.108 kb on + strand at 483.108 kb on + strand at 483.116 kb on - strand at 483.116 kb on - strand at 483.129 kb on + strand at 483.235 kb on + strand at 483.285 kb on + strand at 483.384 kb on - strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.737 kb on + strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.860 kb on + strand, within Shewana3_0424 at 483.872 kb on + strand, within Shewana3_0424 at 483.876 kb on + strand, within Shewana3_0424 at 483.892 kb on - strand, within Shewana3_0424 at 483.960 kb on + strand, within Shewana3_0424 at 484.039 kb on + strand at 484.039 kb on + strand at 484.039 kb on + strand at 484.047 kb on - strand at 484.165 kb on + strand at 484.233 kb on + strand at 484.513 kb on + strand, within pdhR at 484.918 kb on + strand, within pdhR
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 482,438 + +0.6 482,474 - -1.0 482,518 - +0.1 482,539 - +1.2 482,624 - Shewana3_0423 0.20 +1.2 482,637 + Shewana3_0423 0.22 +1.7 482,637 + Shewana3_0423 0.22 +0.4 482,647 + Shewana3_0423 0.24 +1.1 482,655 - Shewana3_0423 0.25 +0.2 482,655 - Shewana3_0423 0.25 +1.2 482,655 - Shewana3_0423 0.25 -0.0 482,655 - Shewana3_0423 0.25 +1.2 482,655 - Shewana3_0423 0.25 +0.1 482,655 - Shewana3_0423 0.25 +0.3 482,655 - Shewana3_0423 0.25 +1.0 482,718 + Shewana3_0423 0.37 +1.3 482,762 + Shewana3_0423 0.45 +0.9 482,812 - Shewana3_0423 0.54 +0.3 482,951 - Shewana3_0423 0.80 +0.7 482,963 - Shewana3_0423 0.82 +0.4 482,990 - Shewana3_0423 0.87 +1.9 483,108 + +1.3 483,108 + +0.4 483,116 - -2.7 483,116 - -1.4 483,129 + +1.0 483,235 + -0.2 483,285 + +0.3 483,384 - Shewana3_0424 0.16 +0.1 483,732 + Shewana3_0424 0.57 +0.1 483,732 + Shewana3_0424 0.57 -0.6 483,737 + Shewana3_0424 0.58 -0.4 483,740 - Shewana3_0424 0.58 -0.7 483,740 - Shewana3_0424 0.58 -1.8 483,740 - Shewana3_0424 0.58 +0.5 483,745 - Shewana3_0424 0.59 +1.5 483,745 - Shewana3_0424 0.59 -0.7 483,745 - Shewana3_0424 0.59 -1.9 483,860 + Shewana3_0424 0.72 +1.3 483,872 + Shewana3_0424 0.73 -0.2 483,876 + Shewana3_0424 0.74 -0.4 483,892 - Shewana3_0424 0.76 -0.3 483,960 + Shewana3_0424 0.84 -0.5 484,039 + -0.0 484,039 + +0.1 484,039 + +0.5 484,047 - -0.2 484,165 + +0.4 484,233 + -0.6 484,513 + pdhR Shewana3_0425 0.12 +2.5 484,918 + pdhR Shewana3_0425 0.66 +0.7
Or see this region's nucleotide sequence