Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabG and Shewana3_0328 are separated by 125 nucleotides Shewana3_0328 and Shewana3_0329 are separated by 5 nucleotides Shewana3_0329 and Shewana3_0330 are separated by 96 nucleotides Shewana3_0330 and Shewana3_0331 are separated by 4 nucleotides
Shewana3_0327: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 361,715 to 362,440
fabG
Shewana3_0328: Shewana3_0328 - thioester dehydrase family protein (RefSeq), at 362,566 to 363,105
_0328
Shewana3_0329: Shewana3_0329 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 363,111 to 364,328
_0329
Shewana3_0330: Shewana3_0330 - hypothetical protein (RefSeq), at 364,425 to 365,159
_0330
Shewana3_0331: Shewana3_0331 - monooxygenase, FAD-binding (RefSeq), at 365,164 to 366,471
_0331
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2 at 362.191 kb on - strand, within fabG at 362.260 kb on + strand, within fabG at 362.302 kb on + strand, within fabG at 362.303 kb on - strand, within fabG at 362.310 kb on - strand, within fabG at 362.420 kb on + strand at 362.501 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.537 kb on - strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.572 kb on - strand at 362.712 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.773 kb on + strand, within Shewana3_0328 at 362.871 kb on - strand, within Shewana3_0328 at 363.171 kb on + strand at 363.298 kb on + strand, within Shewana3_0329 at 363.457 kb on - strand, within Shewana3_0329 at 363.503 kb on + strand, within Shewana3_0329 at 363.567 kb on - strand, within Shewana3_0329 at 363.582 kb on - strand, within Shewana3_0329 at 363.700 kb on + strand, within Shewana3_0329 at 363.708 kb on - strand, within Shewana3_0329 at 363.828 kb on + strand, within Shewana3_0329 at 363.981 kb on + strand, within Shewana3_0329 at 364.015 kb on + strand, within Shewana3_0329 at 364.023 kb on + strand, within Shewana3_0329 at 364.023 kb on + strand, within Shewana3_0329 at 364.056 kb on - strand, within Shewana3_0329 at 364.056 kb on - strand, within Shewana3_0329 at 364.226 kb on - strand at 364.254 kb on + strand at 364.262 kb on - strand at 364.262 kb on - strand at 364.262 kb on - strand at 364.263 kb on - strand at 364.350 kb on + strand at 364.358 kb on - strand at 364.704 kb on - strand, within Shewana3_0330 at 364.773 kb on - strand, within Shewana3_0330 at 364.784 kb on + strand, within Shewana3_0330 at 364.789 kb on + strand, within Shewana3_0330 at 364.789 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 365.009 kb on + strand, within Shewana3_0330 at 365.017 kb on - strand, within Shewana3_0330 at 365.147 kb on + strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 362,191 - fabG Shewana3_0327 0.66 +1.2 362,260 + fabG Shewana3_0327 0.75 -0.4 362,302 + fabG Shewana3_0327 0.81 -1.5 362,303 - fabG Shewana3_0327 0.81 -0.6 362,310 - fabG Shewana3_0327 0.82 -1.5 362,420 + -1.2 362,501 + +0.1 362,529 + -1.0 362,529 + +1.1 362,529 + +1.3 362,537 - +0.5 362,564 + +1.0 362,564 + +0.4 362,564 + +1.6 362,572 - +0.1 362,712 - Shewana3_0328 0.27 -0.2 362,764 - Shewana3_0328 0.37 +0.2 362,764 - Shewana3_0328 0.37 +1.6 362,773 + Shewana3_0328 0.38 +0.2 362,871 - Shewana3_0328 0.56 +0.7 363,171 + +1.3 363,298 + Shewana3_0329 0.15 -0.6 363,457 - Shewana3_0329 0.28 +0.1 363,503 + Shewana3_0329 0.32 +1.5 363,567 - Shewana3_0329 0.37 +0.3 363,582 - Shewana3_0329 0.39 +1.6 363,700 + Shewana3_0329 0.48 -0.2 363,708 - Shewana3_0329 0.49 -0.9 363,828 + Shewana3_0329 0.59 +0.1 363,981 + Shewana3_0329 0.71 -0.2 364,015 + Shewana3_0329 0.74 +0.1 364,023 + Shewana3_0329 0.75 +0.2 364,023 + Shewana3_0329 0.75 -1.8 364,056 - Shewana3_0329 0.78 -2.2 364,056 - Shewana3_0329 0.78 -0.6 364,226 - +0.5 364,254 + -1.5 364,262 - +0.2 364,262 - -0.6 364,262 - +0.7 364,263 - -0.3 364,350 + -0.3 364,358 - -0.6 364,704 - Shewana3_0330 0.38 +0.1 364,773 - Shewana3_0330 0.47 -0.8 364,784 + Shewana3_0330 0.49 -0.4 364,789 + Shewana3_0330 0.50 +0.3 364,789 + Shewana3_0330 0.50 +1.8 364,859 + Shewana3_0330 0.59 -0.3 364,859 + Shewana3_0330 0.59 +0.2 364,859 + Shewana3_0330 0.59 -0.8 364,867 - Shewana3_0330 0.60 +1.2 364,867 - Shewana3_0330 0.60 +1.0 364,867 - Shewana3_0330 0.60 -0.1 365,009 + Shewana3_0330 0.79 -0.6 365,017 - Shewana3_0330 0.81 +0.6 365,147 + -0.9 365,155 - -0.5 365,155 - -0.4 365,155 - +0.5 365,155 - -0.4 365,162 + -0.3
Or see this region's nucleotide sequence