Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnA and Shewana3_0301 are separated by 985 nucleotides Shewana3_0301 and Shewana3_0302 are separated by 85 nucleotides
Shewana3_0300: glnA - glutamine synthetase (RefSeq), at 334,470 to 335,879
glnA
Shewana3_0301: Shewana3_0301 - GTP-binding protein TypA (RefSeq), at 336,865 to 338,676
_0301
Shewana3_0302: Shewana3_0302 - AraC family transcriptional regulator (RefSeq), at 338,762 to 339,769
_0302
Position (kb) 336
337
338
339 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 336.057 kb on - strand at 336.112 kb on - strand at 336.127 kb on + strand at 336.137 kb on - strand at 336.137 kb on - strand at 336.185 kb on - strand at 336.265 kb on + strand at 336.299 kb on + strand at 336.336 kb on + strand at 336.448 kb on + strand at 336.497 kb on - strand at 336.555 kb on + strand at 336.683 kb on + strand at 336.767 kb on + strand at 336.855 kb on + strand at 336.987 kb on + strand at 337.096 kb on - strand, within Shewana3_0301 at 337.131 kb on + strand, within Shewana3_0301 at 337.139 kb on - strand, within Shewana3_0301 at 337.139 kb on - strand, within Shewana3_0301 at 337.139 kb on - strand at 337.223 kb on + strand, within Shewana3_0301 at 337.223 kb on + strand, within Shewana3_0301 at 337.294 kb on + strand, within Shewana3_0301 at 337.547 kb on - strand, within Shewana3_0301 at 337.599 kb on + strand, within Shewana3_0301 at 337.632 kb on - strand, within Shewana3_0301 at 337.632 kb on - strand, within Shewana3_0301 at 337.632 kb on - strand, within Shewana3_0301 at 337.639 kb on + strand, within Shewana3_0301 at 337.647 kb on - strand, within Shewana3_0301 at 337.889 kb on + strand, within Shewana3_0301 at 337.890 kb on - strand, within Shewana3_0301 at 337.919 kb on + strand, within Shewana3_0301 at 337.959 kb on - strand, within Shewana3_0301 at 337.969 kb on - strand, within Shewana3_0301 at 338.185 kb on - strand, within Shewana3_0301 at 338.234 kb on - strand, within Shewana3_0301 at 338.251 kb on + strand, within Shewana3_0301 at 338.259 kb on - strand, within Shewana3_0301 at 338.375 kb on - strand, within Shewana3_0301 at 338.557 kb on - strand at 338.567 kb on + strand at 338.641 kb on + strand at 338.709 kb on - strand at 338.715 kb on + strand at 338.960 kb on + strand, within Shewana3_0302 at 338.960 kb on + strand, within Shewana3_0302 at 338.968 kb on - strand, within Shewana3_0302 at 338.978 kb on - strand, within Shewana3_0302 at 339.008 kb on + strand, within Shewana3_0302 at 339.027 kb on - strand, within Shewana3_0302 at 339.045 kb on + strand, within Shewana3_0302 at 339.182 kb on - strand, within Shewana3_0302 at 339.189 kb on + strand, within Shewana3_0302 at 339.189 kb on + strand, within Shewana3_0302 at 339.206 kb on + strand, within Shewana3_0302 at 339.243 kb on - strand, within Shewana3_0302 at 339.271 kb on - strand, within Shewana3_0302 at 339.381 kb on - strand, within Shewana3_0302 at 339.594 kb on - strand, within Shewana3_0302
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 336,057 - +1.0 336,112 - +1.7 336,127 + +1.2 336,137 - +0.6 336,137 - +0.7 336,185 - +0.9 336,265 + -0.1 336,299 + +0.6 336,336 + +0.2 336,448 + -0.1 336,497 - -1.2 336,555 + +1.3 336,683 + +1.8 336,767 + +0.0 336,855 + -4.8 336,987 + +1.7 337,096 - Shewana3_0301 0.13 +0.2 337,131 + Shewana3_0301 0.15 +1.7 337,139 - Shewana3_0301 0.15 -1.9 337,139 - Shewana3_0301 0.15 -0.0 337,139 - +2.1 337,223 + Shewana3_0301 0.20 +0.5 337,223 + Shewana3_0301 0.20 +1.6 337,294 + Shewana3_0301 0.24 +1.5 337,547 - Shewana3_0301 0.38 -1.6 337,599 + Shewana3_0301 0.41 +1.1 337,632 - Shewana3_0301 0.42 -0.2 337,632 - Shewana3_0301 0.42 -0.2 337,632 - Shewana3_0301 0.42 -0.2 337,639 + Shewana3_0301 0.43 +3.0 337,647 - Shewana3_0301 0.43 -0.4 337,889 + Shewana3_0301 0.57 +1.8 337,890 - Shewana3_0301 0.57 +1.6 337,919 + Shewana3_0301 0.58 +1.0 337,959 - Shewana3_0301 0.60 -0.2 337,969 - Shewana3_0301 0.61 -1.9 338,185 - Shewana3_0301 0.73 +0.8 338,234 - Shewana3_0301 0.76 -1.2 338,251 + Shewana3_0301 0.76 +1.8 338,259 - Shewana3_0301 0.77 -0.8 338,375 - Shewana3_0301 0.83 -0.4 338,557 - -1.2 338,567 + +2.1 338,641 + +1.9 338,709 - -0.6 338,715 + +0.5 338,960 + Shewana3_0302 0.20 -0.3 338,960 + Shewana3_0302 0.20 +0.2 338,968 - Shewana3_0302 0.20 -0.4 338,978 - Shewana3_0302 0.21 -0.1 339,008 + Shewana3_0302 0.24 +1.1 339,027 - Shewana3_0302 0.26 +0.1 339,045 + Shewana3_0302 0.28 -1.6 339,182 - Shewana3_0302 0.42 +1.1 339,189 + Shewana3_0302 0.42 -1.9 339,189 + Shewana3_0302 0.42 +0.2 339,206 + Shewana3_0302 0.44 +1.2 339,243 - Shewana3_0302 0.48 +2.5 339,271 - Shewana3_0302 0.50 -1.2 339,381 - Shewana3_0302 0.61 -0.1 339,594 - Shewana3_0302 0.83 -0.7
Or see this region's nucleotide sequence