Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0168 and nudE are separated by 237 nucleotides nudE and Shewana3_0170 are separated by 70 nucleotides Shewana3_0170 and Shewana3_0171 are separated by 6 nucleotides
Shewana3_0168: Shewana3_0168 - Ig family protein (RefSeq), at 191,630 to 202,264
_0168
Shewana3_0169: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 202,502 to 203,053
nudE
Shewana3_0170: Shewana3_0170 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 203,124 to 203,936
_0170
Shewana3_0171: Shewana3_0171 - GCN5-related N-acetyltransferase (RefSeq), at 203,943 to 204,386
_0171
Position (kb) 202
203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 201.605 kb on + strand at 201.628 kb on - strand at 201.641 kb on + strand at 201.704 kb on - strand at 201.813 kb on - strand at 201.831 kb on - strand at 201.844 kb on + strand at 201.999 kb on + strand at 202.027 kb on + strand at 202.118 kb on + strand at 202.187 kb on + strand at 202.470 kb on - strand at 202.602 kb on - strand, within nudE at 202.803 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.887 kb on + strand, within nudE at 202.978 kb on - strand, within nudE at 203.319 kb on - strand, within Shewana3_0170 at 203.319 kb on - strand, within Shewana3_0170 at 203.319 kb on - strand, within Shewana3_0170 at 203.346 kb on - strand, within Shewana3_0170 at 203.424 kb on + strand, within Shewana3_0170 at 203.685 kb on + strand, within Shewana3_0170 at 203.685 kb on + strand, within Shewana3_0170 at 203.700 kb on + strand, within Shewana3_0170 at 203.700 kb on + strand, within Shewana3_0170 at 203.824 kb on - strand, within Shewana3_0170
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 201,605 + -0.0 201,628 - -0.0 201,641 + -0.1 201,704 - -1.7 201,813 - -1.6 201,831 - -0.8 201,844 + +0.1 201,999 + -0.1 202,027 + -0.4 202,118 + -0.1 202,187 + +0.0 202,470 - +0.3 202,602 - nudE Shewana3_0169 0.18 +2.2 202,803 + nudE Shewana3_0169 0.55 +1.4 202,818 + nudE Shewana3_0169 0.57 +0.4 202,818 + nudE Shewana3_0169 0.57 +1.1 202,887 + nudE Shewana3_0169 0.70 +0.4 202,978 - nudE Shewana3_0169 0.86 -3.0 203,319 - Shewana3_0170 0.24 -1.0 203,319 - Shewana3_0170 0.24 +0.7 203,319 - Shewana3_0170 0.24 -1.7 203,346 - Shewana3_0170 0.27 -2.6 203,424 + Shewana3_0170 0.37 +0.9 203,685 + Shewana3_0170 0.69 +0.2 203,685 + Shewana3_0170 0.69 -0.3 203,700 + Shewana3_0170 0.71 -4.3 203,700 + Shewana3_0170 0.71 -3.3 203,824 - Shewana3_0170 0.86 -0.9
Or see this region's nucleotide sequence