Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0086 and Shewana3_0087 are separated by 71 nucleotides Shewana3_0087 and Shewana3_0088 are separated by 15 nucleotides Shewana3_0088 and Shewana3_0089 are separated by 62 nucleotides
Shewana3_0086: Shewana3_0086 - MerR family transcriptional regulator (RefSeq), at 99,057 to 99,410
_0086
Shewana3_0087: Shewana3_0087 - NAD(P)H dehydrogenase (quinone) (RefSeq), at 99,482 to 100,060
_0087
Shewana3_0088: Shewana3_0088 - glutathione-dependent formaldehyde-activating, GFA (RefSeq), at 100,076 to 100,471
_0088
Shewana3_0089: Shewana3_0089 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (RefSeq), at 100,534 to 101,214
_0089
Position (kb) 100
101 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 99.126 kb on + strand, within Shewana3_0086 at 99.189 kb on - strand, within Shewana3_0086 at 99.267 kb on - strand, within Shewana3_0086 at 99.288 kb on + strand, within Shewana3_0086 at 99.449 kb on - strand at 99.449 kb on - strand at 99.751 kb on + strand, within Shewana3_0087 at 99.817 kb on - strand, within Shewana3_0087 at 100.097 kb on + strand at 100.153 kb on - strand, within Shewana3_0088 at 100.153 kb on - strand, within Shewana3_0088 at 100.170 kb on - strand, within Shewana3_0088 at 100.219 kb on + strand, within Shewana3_0088 at 100.525 kb on + strand at 100.605 kb on + strand, within Shewana3_0089 at 100.605 kb on + strand, within Shewana3_0089 at 100.640 kb on + strand, within Shewana3_0089 at 100.640 kb on + strand, within Shewana3_0089 at 100.694 kb on - strand, within Shewana3_0089 at 100.728 kb on - strand, within Shewana3_0089 at 100.827 kb on - strand, within Shewana3_0089 at 100.903 kb on + strand, within Shewana3_0089 at 100.903 kb on + strand, within Shewana3_0089 at 100.935 kb on - strand, within Shewana3_0089 at 100.969 kb on - strand, within Shewana3_0089 at 101.080 kb on + strand, within Shewana3_0089 at 101.122 kb on + strand, within Shewana3_0089 at 101.122 kb on + strand, within Shewana3_0089 at 101.169 kb on - strand at 101.188 kb on + strand at 101.196 kb on - strand at 101.268 kb on + strand at 101.325 kb on + strand at 101.388 kb on - strand at 101.420 kb on + strand at 101.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 99,126 + Shewana3_0086 0.19 -1.0 99,189 - Shewana3_0086 0.37 -4.8 99,267 - Shewana3_0086 0.59 -0.6 99,288 + Shewana3_0086 0.65 +0.4 99,449 - -0.3 99,449 - +0.5 99,751 + Shewana3_0087 0.46 +0.2 99,817 - Shewana3_0087 0.58 -0.1 100,097 + +0.4 100,153 - Shewana3_0088 0.19 -0.8 100,153 - Shewana3_0088 0.19 -0.6 100,170 - Shewana3_0088 0.24 +0.1 100,219 + Shewana3_0088 0.36 -0.2 100,525 + -1.1 100,605 + Shewana3_0089 0.10 -1.7 100,605 + Shewana3_0089 0.10 -1.4 100,640 + Shewana3_0089 0.16 +0.1 100,640 + Shewana3_0089 0.16 -0.6 100,694 - Shewana3_0089 0.23 -0.0 100,728 - Shewana3_0089 0.28 +0.1 100,827 - Shewana3_0089 0.43 +0.1 100,903 + Shewana3_0089 0.54 -1.2 100,903 + Shewana3_0089 0.54 -0.9 100,935 - Shewana3_0089 0.59 -0.5 100,969 - Shewana3_0089 0.64 -0.7 101,080 + Shewana3_0089 0.80 -1.2 101,122 + Shewana3_0089 0.86 -1.3 101,122 + Shewana3_0089 0.86 -2.3 101,169 - -1.0 101,188 + -0.3 101,196 - -0.4 101,268 + +0.5 101,325 + -0.4 101,388 - -0.1 101,420 + -0.6 101,428 - -0.3
Or see this region's nucleotide sequence