Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyS and glyQ are separated by 9 nucleotides glyQ and Shewana3_0018 are separated by 118 nucleotides Shewana3_0018 and Shewana3_0019 are separated by 85 nucleotides
Shewana3_0016: glyS - glycyl-tRNA synthetase subunit beta (RefSeq), at 18,813 to 20,879
glyS
Shewana3_0017: glyQ - glycyl-tRNA synthetase subunit alpha (RefSeq), at 20,889 to 21,794
glyQ
Shewana3_0018: Shewana3_0018 - DNA-3-methyladenine glycosylase I (RefSeq), at 21,913 to 22,488
_0018
Shewana3_0019: Shewana3_0019 - amidohydrolase (RefSeq), at 22,574 to 25,762
_0019
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 21.895 kb on + strand at 21.895 kb on + strand at 21.976 kb on + strand, within Shewana3_0018 at 21.976 kb on + strand, within Shewana3_0018 at 22.020 kb on + strand, within Shewana3_0018 at 22.025 kb on + strand, within Shewana3_0018 at 22.226 kb on - strand, within Shewana3_0018 at 22.271 kb on + strand, within Shewana3_0018 at 22.313 kb on + strand, within Shewana3_0018 at 22.340 kb on + strand, within Shewana3_0018 at 22.353 kb on + strand, within Shewana3_0018 at 22.353 kb on + strand, within Shewana3_0018 at 22.541 kb on + strand at 22.674 kb on - strand at 22.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 21,895 + +1.6 21,895 + -1.0 21,976 + Shewana3_0018 0.11 +0.2 21,976 + Shewana3_0018 0.11 -0.2 22,020 + Shewana3_0018 0.19 -0.3 22,025 + Shewana3_0018 0.19 -0.9 22,226 - Shewana3_0018 0.54 -1.4 22,271 + Shewana3_0018 0.62 -1.0 22,313 + Shewana3_0018 0.69 +0.1 22,340 + Shewana3_0018 0.74 -0.2 22,353 + Shewana3_0018 0.76 -0.3 22,353 + Shewana3_0018 0.76 +0.3 22,541 + -2.0 22,674 - +0.5 22,684 - +0.5
Or see this region's nucleotide sequence