Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4346 and Shewana3_4347 overlap by 4 nucleotides Shewana3_4347 and Shewana3_4348 are separated by 65 nucleotides Shewana3_4348 and Shewana3_4349 are separated by 178 nucleotides Shewana3_4349 and Shewana3_4350 are separated by 555 nucleotides
Shewana3_4346: Shewana3_4346 - transcriptional regulator MerD (RefSeq), at 232,097 to 232,462
_4346
Shewana3_4347: Shewana3_4347 - putative mercury resistance protein (RefSeq), at 232,459 to 232,695
_4347
Shewana3_4348: Shewana3_4348 - hypothetical protein (RefSeq), at 232,761 to 232,973
_4348
Shewana3_4349: Shewana3_4349 - umuC protein (RefSeq), at 233,152 to 233,817
_4349
Shewana3_4350: Shewana3_4350 - hypothetical protein (RefSeq), at 234,373 to 235,905
_4350
Position (kb) 233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 232.439 kb on - strand at 232.464 kb on - strand at 232.616 kb on - strand, within Shewana3_4347 at 232.651 kb on - strand, within Shewana3_4347 at 232.732 kb on - strand at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.987 kb on - strand at 233.069 kb on - strand at 233.084 kb on - strand at 233.194 kb on + strand at 233.391 kb on + strand, within Shewana3_4349 at 233.563 kb on + strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.720 kb on - strand, within Shewana3_4349 at 233.763 kb on + strand at 233.776 kb on - strand at 233.783 kb on - strand at 233.858 kb on - strand at 233.901 kb on - strand at 234.059 kb on + strand at 234.135 kb on + strand at 234.143 kb on - strand at 234.143 kb on - strand at 234.473 kb on + strand at 234.481 kb on - strand at 234.539 kb on + strand, within Shewana3_4350 at 234.547 kb on - strand, within Shewana3_4350 at 234.549 kb on + strand, within Shewana3_4350 at 234.557 kb on - strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.583 kb on - strand, within Shewana3_4350 at 234.583 kb on - strand, within Shewana3_4350 at 234.642 kb on + strand, within Shewana3_4350 at 234.652 kb on + strand, within Shewana3_4350 at 234.652 kb on + strand, within Shewana3_4350 at 234.661 kb on - strand, within Shewana3_4350 at 234.671 kb on - strand, within Shewana3_4350 at 234.761 kb on - strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 232,439 - -0.1 232,464 - -1.5 232,616 - Shewana3_4347 0.66 +1.0 232,651 - Shewana3_4347 0.81 +0.4 232,732 - +1.1 232,910 + Shewana3_4348 0.70 -0.3 232,910 + Shewana3_4348 0.70 -0.6 232,910 + Shewana3_4348 0.70 -0.8 232,987 - -0.8 233,069 - -0.7 233,084 - +0.2 233,194 + -1.4 233,391 + Shewana3_4349 0.36 -1.9 233,563 + Shewana3_4349 0.62 -0.7 233,571 - Shewana3_4349 0.63 -0.7 233,571 - Shewana3_4349 0.63 +2.4 233,712 + Shewana3_4349 0.84 +0.3 233,712 + Shewana3_4349 0.84 +0.1 233,712 + Shewana3_4349 0.84 -0.3 233,712 + Shewana3_4349 0.84 -0.5 233,712 + Shewana3_4349 0.84 +0.2 233,712 + Shewana3_4349 0.84 -0.5 233,720 - Shewana3_4349 0.85 -0.1 233,763 + +0.8 233,776 - -0.6 233,783 - -0.2 233,858 - +1.1 233,901 - +0.7 234,059 + -1.4 234,135 + +0.2 234,143 - -0.5 234,143 - -0.2 234,473 + -0.2 234,481 - -1.7 234,539 + Shewana3_4350 0.11 -0.8 234,547 - Shewana3_4350 0.11 +0.2 234,549 + Shewana3_4350 0.11 -0.5 234,557 - Shewana3_4350 0.12 -1.0 234,565 + Shewana3_4350 0.13 -3.3 234,565 + Shewana3_4350 0.13 -1.0 234,565 + Shewana3_4350 0.13 +0.8 234,565 + Shewana3_4350 0.13 -1.8 234,573 - Shewana3_4350 0.13 +0.2 234,573 - Shewana3_4350 0.13 +0.9 234,573 - Shewana3_4350 0.13 +1.5 234,583 - Shewana3_4350 0.14 +1.0 234,583 - Shewana3_4350 0.14 -0.5 234,642 + Shewana3_4350 0.18 -0.2 234,652 + Shewana3_4350 0.18 +0.8 234,652 + Shewana3_4350 0.18 +0.9 234,661 - Shewana3_4350 0.19 -1.7 234,671 - Shewana3_4350 0.19 -0.4 234,761 - Shewana3_4350 0.25 -1.0 234,771 + Shewana3_4350 0.26 -3.1 234,771 + Shewana3_4350 0.26 -0.3 234,771 + Shewana3_4350 0.26 +1.2 234,771 + Shewana3_4350 0.26 +0.2 234,779 - Shewana3_4350 0.26 -0.8 234,779 - Shewana3_4350 0.26 -0.6 234,779 - Shewana3_4350 0.26 -0.8 234,779 - Shewana3_4350 0.26 -1.4
Or see this region's nucleotide sequence