Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4345 and Shewana3_4346 are separated by 17 nucleotides Shewana3_4346 and Shewana3_4347 overlap by 4 nucleotides Shewana3_4347 and Shewana3_4348 are separated by 65 nucleotides Shewana3_4348 and Shewana3_4349 are separated by 178 nucleotides
Shewana3_4345: Shewana3_4345 - putative mercuric reductase (RefSeq), at 230,394 to 232,079
_4345
Shewana3_4346: Shewana3_4346 - transcriptional regulator MerD (RefSeq), at 232,097 to 232,462
_4346
Shewana3_4347: Shewana3_4347 - putative mercury resistance protein (RefSeq), at 232,459 to 232,695
_4347
Shewana3_4348: Shewana3_4348 - hypothetical protein (RefSeq), at 232,761 to 232,973
_4348
Shewana3_4349: Shewana3_4349 - umuC protein (RefSeq), at 233,152 to 233,817
_4349
Position (kb) 232
233 Strain fitness (log2 ratio) -2
-1
0
1
2 at 231.602 kb on - strand, within Shewana3_4345 at 231.602 kb on - strand, within Shewana3_4345 at 231.751 kb on - strand, within Shewana3_4345 at 231.754 kb on - strand, within Shewana3_4345 at 232.104 kb on + strand at 232.439 kb on - strand at 232.464 kb on - strand at 232.616 kb on - strand, within Shewana3_4347 at 232.651 kb on - strand, within Shewana3_4347 at 232.732 kb on - strand at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.987 kb on - strand at 233.069 kb on - strand at 233.084 kb on - strand at 233.194 kb on + strand at 233.391 kb on + strand, within Shewana3_4349 at 233.563 kb on + strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 231,602 - Shewana3_4345 0.72 -0.1 231,602 - Shewana3_4345 0.72 +0.1 231,751 - Shewana3_4345 0.80 -0.4 231,754 - Shewana3_4345 0.81 -0.8 232,104 + -0.4 232,439 - -0.1 232,464 - -1.5 232,616 - Shewana3_4347 0.66 +1.0 232,651 - Shewana3_4347 0.81 +0.4 232,732 - +1.1 232,910 + Shewana3_4348 0.70 -0.3 232,910 + Shewana3_4348 0.70 -0.6 232,910 + Shewana3_4348 0.70 -0.8 232,987 - -0.8 233,069 - -0.7 233,084 - +0.2 233,194 + -1.4 233,391 + Shewana3_4349 0.36 -1.9 233,563 + Shewana3_4349 0.62 -0.7 233,571 - Shewana3_4349 0.63 -0.7 233,571 - Shewana3_4349 0.63 +2.4
Or see this region's nucleotide sequence