Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4175 and Shewana3_4176 are separated by 414 nucleotides Shewana3_4176 and Shewana3_4177 overlap by 26 nucleotides
Shewana3_4175: Shewana3_4175 - hypothetical protein (RefSeq), at 41,152 to 42,258
_4175
Shewana3_4176: Shewana3_4176 - hypothetical protein (RefSeq), at 42,673 to 43,521
_4176
Shewana3_4177: Shewana3_4177 - CBS domain-containing protein (RefSeq), at 43,496 to 44,170
_4177
Position (kb) 42
43
44 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 41.863 kb on + strand, within Shewana3_4175 at 41.917 kb on + strand, within Shewana3_4175 at 42.066 kb on + strand, within Shewana3_4175 at 42.122 kb on + strand, within Shewana3_4175 at 42.125 kb on - strand, within Shewana3_4175 at 42.383 kb on + strand at 42.391 kb on - strand at 42.391 kb on - strand at 42.435 kb on + strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.629 kb on + strand at 42.630 kb on + strand at 42.630 kb on + strand at 42.650 kb on - strand at 42.765 kb on - strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176 at 43.284 kb on + strand, within Shewana3_4176 at 43.561 kb on - strand at 43.566 kb on - strand, within Shewana3_4177 at 43.821 kb on - strand, within Shewana3_4177 at 43.827 kb on - strand, within Shewana3_4177 at 43.849 kb on + strand, within Shewana3_4177 at 44.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 41,863 + Shewana3_4175 0.64 +0.3 41,917 + Shewana3_4175 0.69 +1.9 42,066 + Shewana3_4175 0.83 +1.6 42,122 + Shewana3_4175 0.88 -0.8 42,125 - Shewana3_4175 0.88 +0.2 42,383 + -0.4 42,391 - +2.2 42,391 - -1.0 42,435 + -1.2 42,443 - +0.2 42,443 - +1.3 42,443 - -0.7 42,629 + -1.4 42,630 + +1.8 42,630 + -3.8 42,650 - +1.1 42,765 - Shewana3_4176 0.11 +0.6 43,253 + Shewana3_4176 0.68 -1.7 43,253 + Shewana3_4176 0.68 -0.5 43,284 + Shewana3_4176 0.72 -1.8 43,561 - -1.1 43,566 - Shewana3_4177 0.10 +1.4 43,821 - Shewana3_4177 0.48 -0.7 43,827 - Shewana3_4177 0.49 +0.9 43,849 + Shewana3_4177 0.52 -2.1 44,367 - +0.8
Or see this region's nucleotide sequence