Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17665 and BPHYT_RS17670 are separated by 94 nucleotides BPHYT_RS17670 and BPHYT_RS17675 overlap by 4 nucleotides BPHYT_RS17675 and BPHYT_RS17680 are separated by 4 nucleotides BPHYT_RS17680 and BPHYT_RS17685 are separated by 237 nucleotides
BPHYT_RS17665: BPHYT_RS17665 - membrane protein, at 3,994,175 to 3,994,570
_RS17665
BPHYT_RS17670: BPHYT_RS17670 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 3,994,665 to 3,995,060
_RS17670
BPHYT_RS17675: BPHYT_RS17675 - phosphoribosyl-AMP cyclohydrolase, at 3,995,057 to 3,995,464
_RS17675
BPHYT_RS17680: BPHYT_RS17680 - imidazole glycerol phosphate synthase subunit HisF, at 3,995,469 to 3,996,242
_RS17680
BPHYT_RS17685: BPHYT_RS17685 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 3,996,480 to 3,997,232
_RS17685
Position (kb)
3995
3996
3997 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3994.473 kb on + strand, within BPHYT_RS17665 at 3994.492 kb on + strand, within BPHYT_RS17665 at 3994.549 kb on - strand at 3994.660 kb on + strand at 3994.948 kb on + strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.305 kb on - strand, within BPHYT_RS17675 at 3995.536 kb on + strand at 3995.666 kb on + strand, within BPHYT_RS17680 at 3995.881 kb on + strand, within BPHYT_RS17680 at 3995.882 kb on - strand, within BPHYT_RS17680 at 3995.882 kb on - strand, within BPHYT_RS17680 at 3996.230 kb on + strand at 3996.230 kb on + strand at 3996.235 kb on - strand at 3996.235 kb on - strand at 3996.482 kb on - strand at 3996.482 kb on - strand at 3996.733 kb on + strand, within BPHYT_RS17685 at 3996.754 kb on + strand, within BPHYT_RS17685 at 3996.755 kb on - strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.952 kb on + strand, within BPHYT_RS17685 at 3996.953 kb on - strand, within BPHYT_RS17685 at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.189 kb on - strand at 3997.232 kb on + strand at 3997.232 kb on + strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,994,473 + BPHYT_RS17665 0.75 -0.1 3,994,492 + BPHYT_RS17665 0.80 +0.8 3,994,549 - -0.3 3,994,660 + +1.0 3,994,948 + BPHYT_RS17670 0.71 -1.9 3,994,949 - BPHYT_RS17670 0.72 -4.3 3,994,949 - BPHYT_RS17670 0.72 +1.5 3,994,949 - BPHYT_RS17670 0.72 -4.9 3,995,009 + BPHYT_RS17670 0.87 -1.1 3,995,009 + BPHYT_RS17670 0.87 -4.2 3,995,064 + -3.6 3,995,064 + -5.2 3,995,064 + -2.9 3,995,064 + -2.9 3,995,064 + -0.1 3,995,065 - +1.3 3,995,065 - +0.2 3,995,065 - +0.3 3,995,065 - +0.7 3,995,305 - BPHYT_RS17675 0.61 -6.3 3,995,536 + -5.3 3,995,666 + BPHYT_RS17680 0.25 -3.9 3,995,881 + BPHYT_RS17680 0.53 -3.5 3,995,882 - BPHYT_RS17680 0.53 -3.7 3,995,882 - BPHYT_RS17680 0.53 -5.1 3,996,230 + -2.0 3,996,230 + -4.3 3,996,235 - -5.5 3,996,235 - -2.3 3,996,482 - +0.2 3,996,482 - +0.1 3,996,733 + BPHYT_RS17685 0.34 -1.1 3,996,754 + BPHYT_RS17685 0.36 -4.8 3,996,755 - BPHYT_RS17685 0.37 -3.2 3,996,857 + BPHYT_RS17685 0.50 -3.3 3,996,857 + BPHYT_RS17685 0.50 -3.7 3,996,932 - BPHYT_RS17685 0.60 -6.0 3,996,932 - BPHYT_RS17685 0.60 -3.6 3,996,932 - BPHYT_RS17685 0.60 -1.3 3,996,952 + BPHYT_RS17685 0.63 -4.3 3,996,953 - BPHYT_RS17685 0.63 -5.0 3,997,188 + -6.1 3,997,188 + -3.7 3,997,188 + +0.5 3,997,189 - -4.2 3,997,232 + -3.7 3,997,232 + -5.4 3,997,233 - -3.5 3,997,233 - -3.6 3,997,233 - -5.3 3,997,233 - -4.6 3,997,233 - -2.6
Or see this region's nucleotide sequence