Experiment: 5-Hydroxymethylfurfural 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3808 and Ac3H11_3809 overlap by 4 nucleotides Ac3H11_3809 and Ac3H11_3810 are separated by 175 nucleotides Ac3H11_3810 and Ac3H11_3811 are separated by 68 nucleotides
Ac3H11_3808 - tungsten-containing formate dehydrogenase alpha subunit, at 20,822 to 23,707
_3808
Ac3H11_3809 - tungsten-containing formate dehydrogenase beta subunit, at 23,704 to 25,611
_3809
Ac3H11_3810 - Transcriptional regulator, TetR family, at 25,787 to 26,407
_3810
Ac3H11_3811 - putative membrane protein, at 26,476 to 26,883
_3811
Position (kb)
23
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 22.720 kb on - strand, within Ac3H11_3808 at 22.791 kb on + strand, within Ac3H11_3808 at 22.792 kb on - strand, within Ac3H11_3808 at 22.887 kb on + strand, within Ac3H11_3808 at 22.887 kb on + strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 22.888 kb on - strand, within Ac3H11_3808 at 23.580 kb on + strand at 23.581 kb on - strand at 23.804 kb on + strand at 23.804 kb on + strand at 23.805 kb on - strand at 24.155 kb on + strand, within Ac3H11_3809 at 24.155 kb on + strand, within Ac3H11_3809 at 24.156 kb on - strand, within Ac3H11_3809 at 24.156 kb on - strand, within Ac3H11_3809 at 24.156 kb on - strand, within Ac3H11_3809 at 24.156 kb on - strand, within Ac3H11_3809 at 24.161 kb on + strand, within Ac3H11_3809 at 24.161 kb on + strand, within Ac3H11_3809 at 24.161 kb on + strand, within Ac3H11_3809 at 24.162 kb on - strand, within Ac3H11_3809 at 24.162 kb on - strand, within Ac3H11_3809 at 24.162 kb on - strand, within Ac3H11_3809 at 24.195 kb on - strand, within Ac3H11_3809 at 24.356 kb on + strand, within Ac3H11_3809 at 24.357 kb on - strand, within Ac3H11_3809 at 24.416 kb on + strand, within Ac3H11_3809 at 24.416 kb on + strand, within Ac3H11_3809 at 24.417 kb on - strand, within Ac3H11_3809 at 24.491 kb on + strand, within Ac3H11_3809 at 24.491 kb on + strand, within Ac3H11_3809 at 24.491 kb on + strand, within Ac3H11_3809 at 24.492 kb on - strand, within Ac3H11_3809 at 24.492 kb on - strand, within Ac3H11_3809 at 24.492 kb on - strand, within Ac3H11_3809 at 24.492 kb on - strand, within Ac3H11_3809 at 24.492 kb on - strand, within Ac3H11_3809 at 24.594 kb on - strand, within Ac3H11_3809 at 24.608 kb on + strand, within Ac3H11_3809 at 24.608 kb on + strand, within Ac3H11_3809 at 24.608 kb on + strand, within Ac3H11_3809 at 24.609 kb on - strand, within Ac3H11_3809 at 24.609 kb on - strand, within Ac3H11_3809 at 24.609 kb on - strand, within Ac3H11_3809 at 24.609 kb on - strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.612 kb on + strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.613 kb on - strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.614 kb on + strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.615 kb on - strand, within Ac3H11_3809 at 24.689 kb on + strand, within Ac3H11_3809 at 24.689 kb on + strand, within Ac3H11_3809 at 24.911 kb on + strand, within Ac3H11_3809 at 24.911 kb on + strand, within Ac3H11_3809 at 25.310 kb on + strand, within Ac3H11_3809 at 25.311 kb on - strand, within Ac3H11_3809 at 25.311 kb on - strand, within Ac3H11_3809 at 25.311 kb on - strand, within Ac3H11_3809 at 25.328 kb on + strand, within Ac3H11_3809 at 25.328 kb on + strand, within Ac3H11_3809 at 25.328 kb on + strand, within Ac3H11_3809 at 25.329 kb on - strand, within Ac3H11_3809 at 25.421 kb on + strand at 25.643 kb on + strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.659 kb on + strand at 25.659 kb on + strand at 25.768 kb on + strand at 25.768 kb on + strand at 25.769 kb on - strand at 25.769 kb on - strand at 25.769 kb on - strand at 26.121 kb on + strand, within Ac3H11_3810 at 26.122 kb on - strand, within Ac3H11_3810 at 26.376 kb on + strand at 26.376 kb on + strand at 26.377 kb on - strand at 26.377 kb on - strand at 26.377 kb on - strand at 26.399 kb on + strand at 26.400 kb on - strand at 26.400 kb on - strand at 26.433 kb on + strand at 26.433 kb on + strand at 26.434 kb on - strand at 26.447 kb on - strand at 26.457 kb on - strand at 26.471 kb on - strand at 26.471 kb on - strand at 26.502 kb on + strand at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.572 kb on + strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM remove 22,720 - Ac3H11_3808 0.66 -0.5 22,791 + Ac3H11_3808 0.68 -0.3 22,792 - Ac3H11_3808 0.68 -0.2 22,887 + Ac3H11_3808 0.72 -0.3 22,887 + Ac3H11_3808 0.72 -0.9 22,888 - Ac3H11_3808 0.72 -0.7 22,888 - Ac3H11_3808 0.72 +0.2 22,888 - Ac3H11_3808 0.72 -0.5 22,888 - Ac3H11_3808 0.72 -0.9 22,888 - Ac3H11_3808 0.72 +0.1 22,888 - Ac3H11_3808 0.72 +0.7 22,888 - Ac3H11_3808 0.72 -0.1 23,580 + -1.6 23,581 - +0.2 23,804 + +0.4 23,804 + +0.5 23,805 - +1.0 24,155 + Ac3H11_3809 0.24 +0.4 24,155 + Ac3H11_3809 0.24 -0.5 24,156 - Ac3H11_3809 0.24 -1.0 24,156 - Ac3H11_3809 0.24 -0.7 24,156 - Ac3H11_3809 0.24 +1.4 24,156 - Ac3H11_3809 0.24 +0.2 24,161 + Ac3H11_3809 0.24 -0.2 24,161 + Ac3H11_3809 0.24 -0.2 24,161 + Ac3H11_3809 0.24 +0.3 24,162 - Ac3H11_3809 0.24 +0.3 24,162 - Ac3H11_3809 0.24 +0.3 24,162 - Ac3H11_3809 0.24 +1.2 24,195 - Ac3H11_3809 0.26 -0.3 24,356 + Ac3H11_3809 0.34 +0.2 24,357 - Ac3H11_3809 0.34 +1.0 24,416 + Ac3H11_3809 0.37 +1.0 24,416 + Ac3H11_3809 0.37 -0.5 24,417 - Ac3H11_3809 0.37 -0.7 24,491 + Ac3H11_3809 0.41 -0.3 24,491 + Ac3H11_3809 0.41 +0.3 24,491 + Ac3H11_3809 0.41 +0.8 24,492 - Ac3H11_3809 0.41 +0.3 24,492 - Ac3H11_3809 0.41 +0.1 24,492 - Ac3H11_3809 0.41 -0.5 24,492 - Ac3H11_3809 0.41 -0.2 24,492 - Ac3H11_3809 0.41 +0.4 24,594 - Ac3H11_3809 0.47 +0.1 24,608 + Ac3H11_3809 0.47 -0.6 24,608 + Ac3H11_3809 0.47 -0.6 24,608 + Ac3H11_3809 0.47 +0.2 24,609 - Ac3H11_3809 0.47 +1.1 24,609 - Ac3H11_3809 0.47 +1.2 24,609 - Ac3H11_3809 0.47 +1.0 24,609 - Ac3H11_3809 0.47 +0.3 24,612 + Ac3H11_3809 0.48 -0.2 24,612 + Ac3H11_3809 0.48 +1.6 24,612 + Ac3H11_3809 0.48 +0.6 24,612 + Ac3H11_3809 0.48 -0.4 24,612 + Ac3H11_3809 0.48 +0.5 24,612 + Ac3H11_3809 0.48 +0.3 24,612 + Ac3H11_3809 0.48 +0.4 24,612 + Ac3H11_3809 0.48 -0.3 24,612 + Ac3H11_3809 0.48 +0.5 24,613 - Ac3H11_3809 0.48 +0.4 24,613 - Ac3H11_3809 0.48 -1.0 24,613 - Ac3H11_3809 0.48 -0.9 24,613 - Ac3H11_3809 0.48 -0.1 24,613 - Ac3H11_3809 0.48 -1.8 24,613 - Ac3H11_3809 0.48 -0.0 24,613 - Ac3H11_3809 0.48 -0.1 24,614 + Ac3H11_3809 0.48 -0.2 24,614 + Ac3H11_3809 0.48 -0.3 24,614 + Ac3H11_3809 0.48 +0.2 24,614 + Ac3H11_3809 0.48 -0.1 24,614 + Ac3H11_3809 0.48 -2.4 24,614 + Ac3H11_3809 0.48 +0.0 24,614 + Ac3H11_3809 0.48 +0.3 24,614 + Ac3H11_3809 0.48 -0.0 24,614 + Ac3H11_3809 0.48 -1.3 24,614 + Ac3H11_3809 0.48 -0.4 24,614 + Ac3H11_3809 0.48 +0.2 24,614 + Ac3H11_3809 0.48 +0.1 24,614 + Ac3H11_3809 0.48 -1.4 24,614 + Ac3H11_3809 0.48 -0.5 24,614 + Ac3H11_3809 0.48 +0.2 24,614 + Ac3H11_3809 0.48 +1.0 24,614 + Ac3H11_3809 0.48 +0.8 24,614 + Ac3H11_3809 0.48 -1.3 24,614 + Ac3H11_3809 0.48 +0.1 24,614 + Ac3H11_3809 0.48 -1.1 24,614 + Ac3H11_3809 0.48 +1.3 24,614 + Ac3H11_3809 0.48 -0.8 24,614 + Ac3H11_3809 0.48 +0.7 24,614 + Ac3H11_3809 0.48 +0.1 24,614 + Ac3H11_3809 0.48 +0.2 24,614 + Ac3H11_3809 0.48 +0.7 24,614 + Ac3H11_3809 0.48 -2.1 24,614 + Ac3H11_3809 0.48 +0.3 24,615 - Ac3H11_3809 0.48 +0.3 24,615 - Ac3H11_3809 0.48 +1.2 24,615 - Ac3H11_3809 0.48 -0.2 24,615 - Ac3H11_3809 0.48 +0.0 24,615 - Ac3H11_3809 0.48 -0.2 24,615 - Ac3H11_3809 0.48 -0.9 24,615 - Ac3H11_3809 0.48 +1.4 24,615 - Ac3H11_3809 0.48 -0.4 24,615 - Ac3H11_3809 0.48 +0.9 24,615 - Ac3H11_3809 0.48 -0.5 24,615 - Ac3H11_3809 0.48 -0.4 24,615 - Ac3H11_3809 0.48 -0.7 24,615 - Ac3H11_3809 0.48 +0.1 24,615 - Ac3H11_3809 0.48 -0.2 24,615 - Ac3H11_3809 0.48 +0.9 24,615 - Ac3H11_3809 0.48 +1.8 24,615 - Ac3H11_3809 0.48 +0.3 24,615 - Ac3H11_3809 0.48 +0.1 24,615 - Ac3H11_3809 0.48 +0.4 24,615 - Ac3H11_3809 0.48 +0.2 24,615 - Ac3H11_3809 0.48 +0.7 24,615 - Ac3H11_3809 0.48 -1.2 24,615 - Ac3H11_3809 0.48 +0.3 24,615 - Ac3H11_3809 0.48 -0.0 24,689 + Ac3H11_3809 0.52 +0.7 24,689 + Ac3H11_3809 0.52 -0.8 24,911 + Ac3H11_3809 0.63 -0.3 24,911 + Ac3H11_3809 0.63 +0.2 25,310 + Ac3H11_3809 0.84 -1.3 25,311 - Ac3H11_3809 0.84 -0.1 25,311 - Ac3H11_3809 0.84 -0.7 25,311 - Ac3H11_3809 0.84 -0.5 25,328 + Ac3H11_3809 0.85 +0.2 25,328 + Ac3H11_3809 0.85 +0.8 25,328 + Ac3H11_3809 0.85 +0.5 25,329 - Ac3H11_3809 0.85 -0.5 25,421 + -0.5 25,643 + -0.6 25,644 - -0.6 25,644 - -0.6 25,644 - -0.4 25,644 - -0.1 25,644 - +0.5 25,659 + -1.7 25,659 + -0.6 25,768 + -0.4 25,768 + -0.9 25,769 - +0.1 25,769 - -1.3 25,769 - -1.0 26,121 + Ac3H11_3810 0.54 +0.3 26,122 - Ac3H11_3810 0.54 +1.9 26,376 + +0.1 26,376 + -1.2 26,377 - -1.7 26,377 - -0.1 26,377 - -1.1 26,399 + +0.6 26,400 - +0.2 26,400 - -0.4 26,433 + -0.0 26,433 + -0.6 26,434 - -0.6 26,447 - -0.8 26,457 - -1.1 26,471 - -0.3 26,471 - -0.5 26,502 + -0.5 26,560 + Ac3H11_3811 0.21 +0.0 26,560 + Ac3H11_3811 0.21 +1.4 26,560 + Ac3H11_3811 0.21 -1.1 26,560 + Ac3H11_3811 0.21 +0.1 26,561 - Ac3H11_3811 0.21 -0.2 26,561 - Ac3H11_3811 0.21 -0.0 26,561 - Ac3H11_3811 0.21 -0.1 26,561 - Ac3H11_3811 0.21 +1.3 26,572 + Ac3H11_3811 0.24 +0.3 26,573 - Ac3H11_3811 0.24 -0.6 26,573 - Ac3H11_3811 0.24 +0.8 26,573 - Ac3H11_3811 0.24 +0.3
Or see this region's nucleotide sequence