Experiment: A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08170 and ABZR86_RS08175 are separated by 46 nucleotides ABZR86_RS08175 and ABZR86_RS08180 are separated by 10 nucleotides ABZR86_RS08180 and ABZR86_RS08185 overlap by 4 nucleotides ABZR86_RS08185 and ABZR86_RS08190 are separated by 148 nucleotides
ABZR86_RS08170: ABZR86_RS08170 - methionine--tRNA ligase, at 1,820,353 to 1,822,452
_RS08170
ABZR86_RS08175: ABZR86_RS08175 - alpha/beta hydrolase, at 1,822,499 to 1,823,191
_RS08175
ABZR86_RS08180: ABZR86_RS08180 - RnfABCDGE type electron transport complex subunit B, at 1,823,202 to 1,823,816
_RS08180
ABZR86_RS08185: ABZR86_RS08185 - endonuclease III, at 1,823,813 to 1,824,514
_RS08185
ABZR86_RS08190: ABZR86_RS08190 - hypothetical protein, at 1,824,663 to 1,825,142
_RS08190
Position (kb)
1823
1824 Strain fitness (log2 ratio)
-1
0
1
2 at 1822.481 kb on - strand at 1822.568 kb on + strand at 1822.569 kb on - strand, within ABZR86_RS08175 at 1822.616 kb on + strand, within ABZR86_RS08175 at 1822.617 kb on - strand, within ABZR86_RS08175 at 1822.999 kb on - strand, within ABZR86_RS08175 at 1823.163 kb on + strand at 1823.163 kb on + strand at 1823.164 kb on - strand at 1823.164 kb on - strand at 1823.164 kb on - strand at 1823.164 kb on - strand at 1823.164 kb on - strand at 1823.186 kb on + strand at 1823.289 kb on + strand, within ABZR86_RS08180 at 1823.290 kb on - strand, within ABZR86_RS08180 at 1823.888 kb on + strand, within ABZR86_RS08185 at 1823.888 kb on + strand, within ABZR86_RS08185 at 1823.888 kb on + strand, within ABZR86_RS08185 at 1823.888 kb on + strand, within ABZR86_RS08185 at 1823.889 kb on - strand, within ABZR86_RS08185 at 1823.976 kb on - strand, within ABZR86_RS08185 at 1824.036 kb on - strand, within ABZR86_RS08185 at 1824.036 kb on - strand, within ABZR86_RS08185 at 1824.232 kb on + strand, within ABZR86_RS08185 at 1824.232 kb on + strand, within ABZR86_RS08185 at 1824.232 kb on + strand, within ABZR86_RS08185 at 1824.232 kb on + strand, within ABZR86_RS08185 at 1824.232 kb on + strand, within ABZR86_RS08185 at 1824.233 kb on - strand, within ABZR86_RS08185 at 1824.366 kb on - strand, within ABZR86_RS08185 at 1824.502 kb on + strand at 1824.503 kb on - strand at 1824.503 kb on - strand at 1824.520 kb on - strand at 1824.575 kb on - strand at 1824.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.01 mg/ml remove 1,822,481 - -0.1 1,822,568 + -0.9 1,822,569 - ABZR86_RS08175 0.10 -0.1 1,822,616 + ABZR86_RS08175 0.17 -0.1 1,822,617 - ABZR86_RS08175 0.17 +0.1 1,822,999 - ABZR86_RS08175 0.72 +0.5 1,823,163 + -0.8 1,823,163 + -0.1 1,823,164 - -0.7 1,823,164 - -0.1 1,823,164 - +0.8 1,823,164 - +0.1 1,823,164 - -0.4 1,823,186 + -0.6 1,823,289 + ABZR86_RS08180 0.14 +0.2 1,823,290 - ABZR86_RS08180 0.14 -0.2 1,823,888 + ABZR86_RS08185 0.11 -0.3 1,823,888 + ABZR86_RS08185 0.11 -0.8 1,823,888 + ABZR86_RS08185 0.11 -1.0 1,823,888 + ABZR86_RS08185 0.11 -0.5 1,823,889 - ABZR86_RS08185 0.11 -0.3 1,823,976 - ABZR86_RS08185 0.23 +0.3 1,824,036 - ABZR86_RS08185 0.32 -0.2 1,824,036 - ABZR86_RS08185 0.32 +0.4 1,824,232 + ABZR86_RS08185 0.60 -0.1 1,824,232 + ABZR86_RS08185 0.60 -0.2 1,824,232 + ABZR86_RS08185 0.60 -0.1 1,824,232 + ABZR86_RS08185 0.60 -0.3 1,824,232 + ABZR86_RS08185 0.60 +0.7 1,824,233 - ABZR86_RS08185 0.60 +0.3 1,824,366 - ABZR86_RS08185 0.79 +0.5 1,824,502 + +0.6 1,824,503 - -0.1 1,824,503 - -0.0 1,824,520 - +0.7 1,824,575 - +2.3 1,824,592 - -0.3
Or see this region's nucleotide sequence