Experiment: A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05435 and ABZR86_RS05440 are separated by 11 nucleotides ABZR86_RS05440 and ABZR86_RS05445 are separated by 198 nucleotides ABZR86_RS05445 and ABZR86_RS05450 are separated by 80 nucleotides ABZR86_RS05450 and ABZR86_RS05455 overlap by 11 nucleotides
ABZR86_RS05435: ABZR86_RS05435 - outer membrane protein assembly factor BamB, at 1,224,676 to 1,225,872
_RS05435
ABZR86_RS05440: ABZR86_RS05440 - tetratricopeptide repeat protein, at 1,225,884 to 1,226,534
_RS05440
ABZR86_RS05445: ABZR86_RS05445 - helix-turn-helix domain-containing protein, at 1,226,733 to 1,227,638
_RS05445
ABZR86_RS05450: ABZR86_RS05450 - type IV pilus biogenesis/stability protein PilW, at 1,227,719 to 1,228,501
_RS05450
ABZR86_RS05455: ABZR86_RS05455 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 1,228,491 to 1,229,627
_RS05455
Position (kb) 1226
1227
1228 Strain fitness (log2 ratio) -1
0
1
2 at 1225.985 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.060 kb on - strand, within ABZR86_RS05440 at 1226.362 kb on + strand, within ABZR86_RS05440 at 1226.362 kb on + strand, within ABZR86_RS05440 at 1226.363 kb on - strand, within ABZR86_RS05440 at 1226.363 kb on - strand, within ABZR86_RS05440 at 1226.491 kb on - strand at 1226.510 kb on - strand at 1226.518 kb on + strand at 1226.518 kb on + strand at 1226.518 kb on + strand at 1226.519 kb on - strand at 1226.651 kb on - strand at 1226.772 kb on + strand at 1226.772 kb on + strand at 1226.772 kb on + strand at 1226.772 kb on + strand at 1226.772 kb on + strand at 1226.772 kb on + strand at 1226.773 kb on - strand at 1226.773 kb on - strand at 1226.803 kb on + strand at 1226.804 kb on - strand at 1226.926 kb on + strand, within ABZR86_RS05445 at 1226.927 kb on - strand, within ABZR86_RS05445 at 1226.927 kb on - strand, within ABZR86_RS05445 at 1226.927 kb on - strand, within ABZR86_RS05445 at 1227.325 kb on + strand, within ABZR86_RS05445 at 1227.325 kb on + strand, within ABZR86_RS05445 at 1227.515 kb on - strand, within ABZR86_RS05445 at 1227.793 kb on - strand at 1227.793 kb on - strand at 1227.793 kb on - strand at 1227.793 kb on - strand at 1227.793 kb on - strand at 1227.793 kb on - strand at 1227.883 kb on - strand, within ABZR86_RS05450 at 1228.077 kb on + strand, within ABZR86_RS05450 at 1228.077 kb on + strand, within ABZR86_RS05450 at 1228.078 kb on - strand, within ABZR86_RS05450 at 1228.078 kb on - strand, within ABZR86_RS05450 at 1228.108 kb on - strand, within ABZR86_RS05450 at 1228.108 kb on - strand, within ABZR86_RS05450 at 1228.347 kb on + strand, within ABZR86_RS05450 at 1228.347 kb on + strand, within ABZR86_RS05450 at 1228.348 kb on - strand, within ABZR86_RS05450 at 1228.348 kb on - strand, within ABZR86_RS05450 at 1228.348 kb on - strand, within ABZR86_RS05450 at 1228.348 kb on - strand, within ABZR86_RS05450 at 1228.348 kb on - strand, within ABZR86_RS05450
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.01 mg/ml remove 1,225,985 - ABZR86_RS05440 0.16 +0.2 1,226,060 - ABZR86_RS05440 0.27 -1.1 1,226,060 - ABZR86_RS05440 0.27 +0.1 1,226,060 - ABZR86_RS05440 0.27 -1.1 1,226,060 - ABZR86_RS05440 0.27 -1.3 1,226,060 - ABZR86_RS05440 0.27 +0.4 1,226,060 - ABZR86_RS05440 0.27 -0.2 1,226,060 - ABZR86_RS05440 0.27 -0.7 1,226,362 + ABZR86_RS05440 0.73 -0.3 1,226,362 + ABZR86_RS05440 0.73 -1.0 1,226,363 - ABZR86_RS05440 0.74 +0.2 1,226,363 - ABZR86_RS05440 0.74 -0.3 1,226,491 - -0.2 1,226,510 - +0.1 1,226,518 + +0.1 1,226,518 + -0.0 1,226,518 + +0.1 1,226,519 - -0.7 1,226,651 - +0.7 1,226,772 + -0.8 1,226,772 + -0.6 1,226,772 + +1.0 1,226,772 + +0.5 1,226,772 + -0.3 1,226,772 + +0.3 1,226,773 - -0.9 1,226,773 - -0.3 1,226,803 + +0.1 1,226,804 - -0.4 1,226,926 + ABZR86_RS05445 0.21 +0.3 1,226,927 - ABZR86_RS05445 0.21 -0.6 1,226,927 - ABZR86_RS05445 0.21 -0.0 1,226,927 - ABZR86_RS05445 0.21 -0.8 1,227,325 + ABZR86_RS05445 0.65 -1.0 1,227,325 + ABZR86_RS05445 0.65 -0.1 1,227,515 - ABZR86_RS05445 0.86 -0.1 1,227,793 - -0.4 1,227,793 - +0.1 1,227,793 - -0.6 1,227,793 - -0.8 1,227,793 - +0.6 1,227,793 - +0.1 1,227,883 - ABZR86_RS05450 0.21 +0.0 1,228,077 + ABZR86_RS05450 0.46 +0.5 1,228,077 + ABZR86_RS05450 0.46 -0.6 1,228,078 - ABZR86_RS05450 0.46 -0.0 1,228,078 - ABZR86_RS05450 0.46 -1.2 1,228,108 - ABZR86_RS05450 0.50 +0.2 1,228,108 - ABZR86_RS05450 0.50 -0.7 1,228,347 + ABZR86_RS05450 0.80 -0.8 1,228,347 + ABZR86_RS05450 0.80 -0.5 1,228,348 - ABZR86_RS05450 0.80 +0.2 1,228,348 - ABZR86_RS05450 0.80 -0.1 1,228,348 - ABZR86_RS05450 0.80 -0.2 1,228,348 - ABZR86_RS05450 0.80 -0.4 1,228,348 - ABZR86_RS05450 0.80 +1.9
Or see this region's nucleotide sequence