Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2420 and DvMF_2421 are separated by 182 nucleotides DvMF_2421 and DvMF_2422 are separated by 97 nucleotides DvMF_2422 and DvMF_2423 overlap by 4 nucleotides
DvMF_2420: DvMF_2420 - hypothetical protein (RefSeq), at 3,058,325 to 3,058,621
_2420
DvMF_2421: DvMF_2421 - hypothetical protein (RefSeq), at 3,058,804 to 3,059,316
_2421
DvMF_2422: DvMF_2422 - type III secretion low calcium response chaperone LcrH/SycD (RefSeq), at 3,059,414 to 3,059,917
_2422
DvMF_2423: DvMF_2423 - hypothetical protein (RefSeq), at 3,059,914 to 3,060,930
_2423
Position (kb)
3058
3059
3060 Strain fitness (log2 ratio)
-1
0
1
2 at 3057.997 kb on + strand at 3058.508 kb on + strand, within DvMF_2420 at 3058.795 kb on - strand at 3059.228 kb on - strand, within DvMF_2421 at 3059.228 kb on - strand, within DvMF_2421 at 3059.228 kb on - strand, within DvMF_2421 at 3059.238 kb on - strand, within DvMF_2421 at 3059.480 kb on - strand, within DvMF_2422 at 3059.515 kb on + strand, within DvMF_2422 at 3059.875 kb on + strand at 3059.877 kb on - strand at 3059.964 kb on - strand at 3060.044 kb on - strand, within DvMF_2423 at 3060.171 kb on + strand, within DvMF_2423 at 3060.195 kb on + strand, within DvMF_2423 at 3060.260 kb on - strand, within DvMF_2423
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 3,057,997 + -0.7 3,058,508 + DvMF_2420 0.62 -1.2 3,058,795 - -1.1 3,059,228 - DvMF_2421 0.83 -0.6 3,059,228 - DvMF_2421 0.83 -0.6 3,059,228 - DvMF_2421 0.83 +1.8 3,059,238 - DvMF_2421 0.85 -0.9 3,059,480 - DvMF_2422 0.13 -0.9 3,059,515 + DvMF_2422 0.20 +0.2 3,059,875 + -1.0 3,059,877 - -0.9 3,059,964 - -0.4 3,060,044 - DvMF_2423 0.13 -0.4 3,060,171 + DvMF_2423 0.25 -0.4 3,060,195 + DvMF_2423 0.28 -0.7 3,060,260 - DvMF_2423 0.34 -0.7
Or see this region's nucleotide sequence