Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0543 and DvMF_0544 are separated by 317 nucleotides DvMF_0544 and DvMF_0545 overlap by 4 nucleotides
DvMF_0543: DvMF_0543 - sodium/hydrogen exchanger (RefSeq), at 638,633 to 640,528
_0543
DvMF_0544: DvMF_0544 - glutamate synthase alpha subunit domain protein (RefSeq), at 640,846 to 641,691
_0544
DvMF_0545: DvMF_0545 - glutamine amidotransferase class-II (RefSeq), at 641,688 to 642,797
_0545
Position (kb) 640
641
642 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 639.885 kb on + strand, within DvMF_0543 at 639.939 kb on - strand, within DvMF_0543 at 639.954 kb on - strand, within DvMF_0543 at 640.185 kb on + strand, within DvMF_0543 at 640.196 kb on + strand, within DvMF_0543 at 640.469 kb on + strand at 640.534 kb on + strand at 640.544 kb on + strand at 640.544 kb on + strand at 640.621 kb on + strand at 640.801 kb on + strand at 640.809 kb on - strand at 640.886 kb on + strand at 640.900 kb on - strand at 641.064 kb on + strand, within DvMF_0544 at 641.106 kb on - strand, within DvMF_0544 at 641.341 kb on - strand, within DvMF_0544 at 641.346 kb on - strand, within DvMF_0544 at 641.573 kb on - strand, within DvMF_0544 at 641.584 kb on - strand, within DvMF_0544 at 641.584 kb on - strand, within DvMF_0544 at 641.647 kb on - strand at 641.840 kb on + strand, within DvMF_0545 at 641.933 kb on - strand, within DvMF_0545 at 641.989 kb on + strand, within DvMF_0545 at 642.033 kb on - strand, within DvMF_0545 at 642.180 kb on - strand, within DvMF_0545 at 642.588 kb on - strand, within DvMF_0545 at 642.598 kb on + strand, within DvMF_0545
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 639,885 + DvMF_0543 0.66 -0.5 639,939 - DvMF_0543 0.69 -0.5 639,954 - DvMF_0543 0.70 -0.5 640,185 + DvMF_0543 0.82 -0.9 640,196 + DvMF_0543 0.82 -0.7 640,469 + -0.2 640,534 + -2.2 640,544 + +0.4 640,544 + -1.2 640,621 + -0.5 640,801 + -0.2 640,809 - -0.3 640,886 + -1.1 640,900 - -0.7 641,064 + DvMF_0544 0.26 -0.7 641,106 - DvMF_0544 0.31 -1.0 641,341 - DvMF_0544 0.59 -2.2 641,346 - DvMF_0544 0.59 -0.7 641,573 - DvMF_0544 0.86 +0.2 641,584 - DvMF_0544 0.87 -0.6 641,584 - DvMF_0544 0.87 +0.9 641,647 - +0.0 641,840 + DvMF_0545 0.14 -1.6 641,933 - DvMF_0545 0.22 -0.7 641,989 + DvMF_0545 0.27 -0.3 642,033 - DvMF_0545 0.31 -1.5 642,180 - DvMF_0545 0.44 -0.5 642,588 - DvMF_0545 0.81 +4.3 642,598 + DvMF_0545 0.82 +0.3
Or see this region's nucleotide sequence