Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0483 and DvMF_0484 are separated by 115 nucleotides DvMF_0484 and DvMF_0485 are separated by 100 nucleotides DvMF_0485 and DvMF_0486 overlap by 4 nucleotides
DvMF_0483: DvMF_0483 - peptidase M48 Ste24p (RefSeq), at 567,426 to 568,898
_0483
DvMF_0484: DvMF_0484 - Appr-1-p processing domain protein (RefSeq), at 569,014 to 569,622
_0484
DvMF_0485: DvMF_0485 - ATP-dependent protease peptidase subunit (RefSeq), at 569,723 to 570,280
_0485
DvMF_0486: DvMF_0486 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (RefSeq), at 570,277 to 571,266
_0486
Position (kb) 569
570
571 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 569.117 kb on - strand, within DvMF_0484 at 569.266 kb on + strand, within DvMF_0484 at 569.360 kb on + strand, within DvMF_0484 at 569.463 kb on + strand, within DvMF_0484 at 569.471 kb on - strand, within DvMF_0484 at 569.471 kb on - strand, within DvMF_0484 at 569.585 kb on - strand at 569.665 kb on + strand at 569.679 kb on - strand at 569.799 kb on + strand, within DvMF_0485 at 569.906 kb on - strand, within DvMF_0485 at 570.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 569,117 - DvMF_0484 0.17 -2.1 569,266 + DvMF_0484 0.41 -0.4 569,360 + DvMF_0484 0.57 +0.4 569,463 + DvMF_0484 0.74 -0.2 569,471 - DvMF_0484 0.75 -0.5 569,471 - DvMF_0484 0.75 -2.8 569,585 - -0.1 569,665 + -0.6 569,679 - +0.2 569,799 + DvMF_0485 0.14 +1.2 569,906 - DvMF_0485 0.33 +1.1 570,296 - +3.2
Or see this region's nucleotide sequence