Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0439 and DvMF_0440 overlap by 4 nucleotides DvMF_0440 and DvMF_0441 are separated by 222 nucleotides DvMF_0441 and DvMF_0442 are separated by 466 nucleotides
DvMF_0439: DvMF_0439 - phosphoglyceromutase (RefSeq), at 514,727 to 516,400
_0439
DvMF_0440: DvMF_0440 - iojap-like protein (RefSeq), at 516,397 to 516,984
_0440
DvMF_0441: DvMF_0441 - nitroreductase (RefSeq), at 517,207 to 517,860
_0441
DvMF_0442: DvMF_0442 - Phenylacetate--CoA ligase (RefSeq), at 518,327 to 519,631
_0442
Position (kb) 517
518 Strain fitness (log2 ratio) -1
0
1 at 516.590 kb on + strand, within DvMF_0440 at 516.800 kb on + strand, within DvMF_0440 at 516.877 kb on + strand, within DvMF_0440 at 516.885 kb on - strand, within DvMF_0440 at 516.997 kb on + strand at 516.998 kb on - strand at 517.019 kb on + strand at 517.027 kb on - strand at 517.204 kb on + strand at 517.212 kb on - strand at 517.384 kb on + strand, within DvMF_0441 at 517.684 kb on - strand, within DvMF_0441 at 517.684 kb on - strand, within DvMF_0441 at 517.864 kb on - strand at 517.940 kb on - strand at 517.967 kb on + strand at 517.967 kb on + strand at 518.251 kb on - strand at 518.455 kb on + strand at 518.769 kb on - strand, within DvMF_0442 at 518.857 kb on + strand, within DvMF_0442
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 516,590 + DvMF_0440 0.33 +0.7 516,800 + DvMF_0440 0.69 +0.5 516,877 + DvMF_0440 0.82 +1.1 516,885 - DvMF_0440 0.83 +0.2 516,997 + -0.3 516,998 - +0.9 517,019 + -0.6 517,027 - -0.7 517,204 + +0.5 517,212 - -0.7 517,384 + DvMF_0441 0.27 -0.6 517,684 - DvMF_0441 0.73 -1.2 517,684 - DvMF_0441 0.73 -0.1 517,864 - -0.1 517,940 - +0.9 517,967 + -0.4 517,967 + -0.3 518,251 - +0.0 518,455 + -0.8 518,769 - DvMF_0442 0.34 -0.9 518,857 + DvMF_0442 0.41 -0.5
Or see this region's nucleotide sequence