Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0389 and DvMF_0390 are separated by 267 nucleotides DvMF_0390 and DvMF_0391 are separated by 311 nucleotides
DvMF_0389: DvMF_0389 - Beta-lactamase (RefSeq), at 457,193 to 458,038
_0389
DvMF_0390: DvMF_0390 - hypothetical protein (RefSeq), at 458,306 to 459,697
_0390
DvMF_0391: DvMF_0391 - Carbonate dehydratase (RefSeq), at 460,009 to 460,665
_0391
Position (kb) 458
459
460 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 457.309 kb on + strand, within DvMF_0389 at 457.400 kb on + strand, within DvMF_0389 at 457.458 kb on + strand, within DvMF_0389 at 457.472 kb on - strand, within DvMF_0389 at 457.534 kb on - strand, within DvMF_0389 at 457.661 kb on - strand, within DvMF_0389 at 458.167 kb on + strand at 458.417 kb on - strand at 458.667 kb on + strand, within DvMF_0390 at 458.675 kb on - strand, within DvMF_0390 at 458.834 kb on - strand, within DvMF_0390 at 458.881 kb on + strand, within DvMF_0390 at 458.898 kb on + strand, within DvMF_0390 at 459.116 kb on - strand, within DvMF_0390 at 459.200 kb on + strand, within DvMF_0390 at 459.216 kb on + strand, within DvMF_0390 at 459.224 kb on - strand, within DvMF_0390 at 459.231 kb on + strand, within DvMF_0390 at 459.231 kb on + strand, within DvMF_0390 at 459.231 kb on + strand, within DvMF_0390 at 459.356 kb on + strand, within DvMF_0390 at 459.502 kb on + strand, within DvMF_0390 at 459.857 kb on - strand at 459.950 kb on - strand at 460.026 kb on + strand at 460.060 kb on + strand at 460.382 kb on - strand, within DvMF_0391 at 460.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 457,309 + DvMF_0389 0.14 +0.6 457,400 + DvMF_0389 0.24 -0.6 457,458 + DvMF_0389 0.31 -0.6 457,472 - DvMF_0389 0.33 +0.1 457,534 - DvMF_0389 0.40 +0.1 457,661 - DvMF_0389 0.55 -2.7 458,167 + -0.4 458,417 - +0.2 458,667 + DvMF_0390 0.26 +0.7 458,675 - DvMF_0390 0.27 +0.5 458,834 - DvMF_0390 0.38 -0.1 458,881 + DvMF_0390 0.41 +0.9 458,898 + DvMF_0390 0.43 +0.5 459,116 - DvMF_0390 0.58 +0.3 459,200 + DvMF_0390 0.64 +0.9 459,216 + DvMF_0390 0.65 +0.6 459,224 - DvMF_0390 0.66 +0.7 459,231 + DvMF_0390 0.66 +0.5 459,231 + DvMF_0390 0.66 +2.4 459,231 + DvMF_0390 0.66 +1.1 459,356 + DvMF_0390 0.75 +0.8 459,502 + DvMF_0390 0.86 +0.9 459,857 - -0.5 459,950 - -0.3 460,026 + -0.1 460,060 + -1.1 460,382 - DvMF_0391 0.57 -0.6 460,688 - +5.3
Or see this region's nucleotide sequence