Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0251 and DvMF_0252 are separated by 121 nucleotides DvMF_0252 and DvMF_0253 overlap by 52 nucleotides DvMF_0253 and DvMF_0254 are separated by 11 nucleotides
DvMF_0251: DvMF_0251 - protein of unknown function DUF342 (RefSeq), at 287,292 to 288,437
_0251
DvMF_0252: DvMF_0252 - anti-sigma-factor antagonist (RefSeq), at 288,559 to 288,903
_0252
DvMF_0253: DvMF_0253 - putative signal transduction histidine kinase (RefSeq), at 288,852 to 289,313
_0253
DvMF_0254: DvMF_0254 - response regulator receiver modulated serine phosphatase (RefSeq), at 289,325 to 290,917
_0254
Position (kb)
288
289 Strain fitness (log2 ratio)
-1
0
1 at 287.712 kb on - strand, within DvMF_0251 at 287.764 kb on + strand, within DvMF_0251 at 287.772 kb on - strand, within DvMF_0251 at 287.904 kb on - strand, within DvMF_0251 at 288.656 kb on - strand, within DvMF_0252 at 288.900 kb on + strand at 288.970 kb on + strand, within DvMF_0253 at 289.111 kb on - strand, within DvMF_0253 at 289.201 kb on - strand, within DvMF_0253 at 289.211 kb on + strand, within DvMF_0253 at 289.381 kb on + strand at 289.424 kb on + strand at 289.635 kb on - strand, within DvMF_0254 at 289.693 kb on - strand, within DvMF_0254 at 289.741 kb on + strand, within DvMF_0254 at 289.758 kb on + strand, within DvMF_0254
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 287,712 - DvMF_0251 0.37 -1.0 287,764 + DvMF_0251 0.41 -0.6 287,772 - DvMF_0251 0.42 -1.2 287,904 - DvMF_0251 0.53 -1.3 288,656 - DvMF_0252 0.28 +0.5 288,900 + -0.5 288,970 + DvMF_0253 0.26 +1.1 289,111 - DvMF_0253 0.56 -0.7 289,201 - DvMF_0253 0.76 -1.6 289,211 + DvMF_0253 0.78 -0.2 289,381 + -0.9 289,424 + -0.8 289,635 - DvMF_0254 0.19 +0.6 289,693 - DvMF_0254 0.23 +0.3 289,741 + DvMF_0254 0.26 -1.5 289,758 + DvMF_0254 0.27 -1.1
Or see this region's nucleotide sequence