Experiment: MoYLS4 with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0130 and DvMF_0131 are separated by 360 nucleotides DvMF_0131 and DvMF_0132 are separated by 2 nucleotides DvMF_0132 and DvMF_0133 are separated by 127 nucleotides
DvMF_0130: DvMF_0130 - DNA polymerase III, alpha subunit (RefSeq), at 143,616 to 147,137
_0130
DvMF_0131: DvMF_0131 - hypothetical protein (RefSeq), at 147,498 to 147,839
_0131
DvMF_0132: DvMF_0132 - metal-dependent phosphohydrolase HD region (RefSeq), at 147,842 to 148,711
_0132
DvMF_0133: DvMF_0133 - Methyltransferase type 11 (RefSeq), at 148,839 to 149,576
_0133
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 147.377 kb on + strand at 147.447 kb on + strand at 147.675 kb on + strand, within DvMF_0131 at 147.795 kb on - strand, within DvMF_0131 at 147.882 kb on - strand at 148.010 kb on + strand, within DvMF_0132 at 148.019 kb on + strand, within DvMF_0132 at 148.048 kb on - strand, within DvMF_0132 at 148.073 kb on - strand, within DvMF_0132 at 148.275 kb on + strand, within DvMF_0132 at 148.364 kb on + strand, within DvMF_0132 at 148.372 kb on - strand, within DvMF_0132 at 148.818 kb on + strand at 148.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.5 mg/ml remove 147,377 + -0.5 147,447 + -0.5 147,675 + DvMF_0131 0.52 -1.8 147,795 - DvMF_0131 0.87 -1.7 147,882 - +1.0 148,010 + DvMF_0132 0.19 -0.9 148,019 + DvMF_0132 0.20 -3.2 148,048 - DvMF_0132 0.24 -0.6 148,073 - DvMF_0132 0.27 -0.3 148,275 + DvMF_0132 0.50 -0.4 148,364 + DvMF_0132 0.60 -0.6 148,372 - DvMF_0132 0.61 +1.3 148,818 + -1.7 148,894 - -0.7
Or see this region's nucleotide sequence