Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotides Pf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides Pf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotides Pf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355
_463
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371
_465
Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066
_466
Position (kb) 529
530
531 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 528.391 kb on - strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.548 kb on + strand at 528.711 kb on + strand at 528.712 kb on - strand at 528.948 kb on + strand, within Pf6N2E2_463 at 528.948 kb on + strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.995 kb on + strand, within Pf6N2E2_463 at 529.131 kb on + strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.406 kb on - strand at 529.420 kb on + strand at 529.421 kb on - strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.472 kb on - strand at 529.484 kb on - strand at 529.774 kb on + strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 530.732 kb on - strand at 530.754 kb on - strand at 530.890 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.966 kb on - strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.404 kb on + strand at 531.404 kb on + strand at 531.405 kb on - strand at 531.405 kb on - strand at 531.434 kb on + strand at 531.435 kb on - strand at 531.435 kb on - strand at 531.615 kb on + strand, within Pf6N2E2_466 at 531.615 kb on + strand, within Pf6N2E2_466 at 531.616 kb on - strand, within Pf6N2E2_466
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 528,391 - -0.4 528,546 + +0.2 528,546 + -0.2 528,546 + +0.4 528,546 + +0.6 528,546 + +0.3 528,548 + +0.9 528,711 + -3.4 528,712 - -3.0 528,948 + Pf6N2E2_463 0.42 -1.6 528,948 + Pf6N2E2_463 0.42 -1.6 528,949 - Pf6N2E2_463 0.42 -3.1 528,949 - Pf6N2E2_463 0.42 -2.4 528,949 - Pf6N2E2_463 0.42 -3.1 528,949 - Pf6N2E2_463 0.42 -0.6 528,949 - Pf6N2E2_463 0.42 -2.4 528,995 + Pf6N2E2_463 0.49 -2.3 529,131 + Pf6N2E2_463 0.68 -1.7 529,132 - Pf6N2E2_463 0.68 -1.2 529,132 - Pf6N2E2_463 0.68 -1.1 529,171 + Pf6N2E2_463 0.74 -1.7 529,171 + Pf6N2E2_463 0.74 -3.1 529,172 - Pf6N2E2_463 0.74 -1.6 529,172 - Pf6N2E2_463 0.74 -2.8 529,172 - Pf6N2E2_463 0.74 -3.6 529,406 - -1.9 529,420 + -3.2 529,421 - -2.2 529,471 + -4.0 529,471 + -1.4 529,471 + -1.4 529,471 + -3.6 529,471 + -2.4 529,471 + -2.4 529,472 - -1.1 529,484 - -3.7 529,774 + Pf6N2E2_464 0.32 -1.2 529,775 - Pf6N2E2_464 0.32 -4.3 529,775 - Pf6N2E2_464 0.32 -1.9 530,732 - -0.6 530,754 - -2.0 530,890 + Pf6N2E2_465 0.25 +0.3 530,905 + Pf6N2E2_465 0.27 +0.7 530,905 + Pf6N2E2_465 0.27 -1.2 530,905 + Pf6N2E2_465 0.27 -0.1 530,906 - Pf6N2E2_465 0.27 -1.0 530,906 - Pf6N2E2_465 0.27 -0.3 530,965 + Pf6N2E2_465 0.37 -0.1 530,965 + Pf6N2E2_465 0.37 +0.6 530,965 + Pf6N2E2_465 0.37 -0.2 530,965 + Pf6N2E2_465 0.37 +1.0 530,966 - Pf6N2E2_465 0.37 +0.0 530,968 + Pf6N2E2_465 0.37 +0.1 530,968 + Pf6N2E2_465 0.37 +1.6 530,969 - Pf6N2E2_465 0.37 -0.2 530,969 - Pf6N2E2_465 0.37 -0.4 531,239 - Pf6N2E2_465 0.79 +1.3 531,239 - Pf6N2E2_465 0.79 +0.3 531,239 - Pf6N2E2_465 0.79 +0.5 531,401 + +0.2 531,401 + -0.5 531,401 + -0.1 531,401 + +0.1 531,401 + +1.4 531,404 + +0.1 531,404 + +0.1 531,405 - +0.4 531,405 - +0.4 531,434 + +0.4 531,435 - +0.1 531,435 - -0.3 531,615 + Pf6N2E2_466 0.31 +0.2 531,615 + Pf6N2E2_466 0.31 -0.5 531,616 - Pf6N2E2_466 0.31 -1.0
Or see this region's nucleotide sequence