Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4616 and Pf6N2E2_4617 are separated by 185 nucleotides Pf6N2E2_4617 and Pf6N2E2_4618 overlap by 8 nucleotides
Pf6N2E2_4616 - type IV pilus biogenesis protein PilJ, at 5,339,627 to 5,341,690
_4616
Pf6N2E2_4617 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 5,341,876 to 5,347,809
_4617
Pf6N2E2_4618 - Chemotaxis signal transduction protein, at 5,347,802 to 5,348,281
_4618
Position (kb) 5341
5342
5343
5344
5345
5346
5347
5348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5340.944 kb on - strand, within Pf6N2E2_4616 at 5340.944 kb on - strand, within Pf6N2E2_4616 at 5341.108 kb on + strand, within Pf6N2E2_4616 at 5341.108 kb on + strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.645 kb on + strand at 5341.646 kb on - strand at 5341.646 kb on - strand at 5341.712 kb on + strand at 5341.712 kb on + strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.780 kb on - strand at 5341.841 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.853 kb on - strand at 5341.853 kb on - strand at 5341.853 kb on - strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5342.056 kb on + strand at 5342.056 kb on + strand at 5342.057 kb on - strand at 5342.179 kb on + strand at 5342.179 kb on + strand at 5342.180 kb on - strand at 5342.180 kb on - strand at 5342.180 kb on - strand at 5342.192 kb on + strand at 5342.193 kb on - strand at 5342.193 kb on - strand at 5342.193 kb on - strand at 5342.450 kb on - strand at 5342.510 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.688 kb on + strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.740 kb on + strand, within Pf6N2E2_4617 at 5342.858 kb on + strand, within Pf6N2E2_4617 at 5343.088 kb on + strand, within Pf6N2E2_4617 at 5343.089 kb on - strand, within Pf6N2E2_4617 at 5343.244 kb on + strand, within Pf6N2E2_4617 at 5343.245 kb on - strand, within Pf6N2E2_4617 at 5343.457 kb on + strand, within Pf6N2E2_4617 at 5343.457 kb on + strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.461 kb on - strand, within Pf6N2E2_4617 at 5343.461 kb on - strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.818 kb on - strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.798 kb on + strand, within Pf6N2E2_4617 at 5344.798 kb on + strand, within Pf6N2E2_4617 at 5344.799 kb on - strand, within Pf6N2E2_4617 at 5344.934 kb on + strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5345.288 kb on - strand, within Pf6N2E2_4617 at 5345.288 kb on - strand at 5345.317 kb on + strand, within Pf6N2E2_4617 at 5345.318 kb on - strand, within Pf6N2E2_4617 at 5345.318 kb on - strand, within Pf6N2E2_4617 at 5345.556 kb on + strand, within Pf6N2E2_4617 at 5345.557 kb on - strand, within Pf6N2E2_4617 at 5345.557 kb on - strand, within Pf6N2E2_4617 at 5345.624 kb on + strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.922 kb on + strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5346.370 kb on - strand, within Pf6N2E2_4617 at 5346.526 kb on - strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.639 kb on + strand, within Pf6N2E2_4617 at 5346.640 kb on - strand, within Pf6N2E2_4617 at 5346.640 kb on - strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.127 kb on - strand, within Pf6N2E2_4617 at 5347.413 kb on - strand at 5347.413 kb on - strand at 5347.738 kb on + strand at 5347.739 kb on - strand at 5347.753 kb on + strand at 5348.123 kb on + strand, within Pf6N2E2_4618 at 5348.124 kb on - strand, within Pf6N2E2_4618 at 5348.148 kb on - strand, within Pf6N2E2_4618 at 5348.148 kb on - strand, within Pf6N2E2_4618 at 5348.205 kb on + strand, within Pf6N2E2_4618 at 5348.205 kb on + strand, within Pf6N2E2_4618 at 5348.206 kb on - strand, within Pf6N2E2_4618 at 5348.206 kb on - strand, within Pf6N2E2_4618 at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.297 kb on - strand at 5348.370 kb on - strand at 5348.370 kb on - strand at 5348.414 kb on + strand at 5348.564 kb on - strand at 5348.626 kb on + strand at 5348.626 kb on + strand at 5348.628 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.656 kb on - strand at 5348.656 kb on - strand at 5348.656 kb on - strand at 5348.717 kb on + strand at 5348.719 kb on + strand at 5348.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 5,340,944 - Pf6N2E2_4616 0.64 -0.2 5,340,944 - Pf6N2E2_4616 0.64 -0.3 5,341,108 + Pf6N2E2_4616 0.72 +0.4 5,341,108 + Pf6N2E2_4616 0.72 +0.6 5,341,109 - Pf6N2E2_4616 0.72 -0.2 5,341,109 - Pf6N2E2_4616 0.72 -0.6 5,341,109 - Pf6N2E2_4616 0.72 +0.2 5,341,645 + -0.7 5,341,646 - -0.1 5,341,646 - -3.3 5,341,712 + -1.2 5,341,712 + -0.3 5,341,713 - +0.0 5,341,713 - -0.2 5,341,713 - -0.5 5,341,713 - +0.2 5,341,713 - -0.1 5,341,713 - -0.2 5,341,713 - -0.7 5,341,780 - -1.5 5,341,841 + +0.3 5,341,852 + -0.7 5,341,852 + +0.1 5,341,852 + +0.1 5,341,852 + -0.1 5,341,852 + +0.1 5,341,852 + -0.8 5,341,852 + +1.2 5,341,853 - +0.1 5,341,853 - +0.7 5,341,853 - -0.6 5,341,894 + +0.1 5,341,894 + +0.2 5,341,894 + +0.6 5,341,894 + +0.5 5,341,894 + -0.0 5,341,894 + +1.5 5,341,894 + +0.7 5,341,895 - -0.8 5,341,895 - -1.1 5,341,895 - -0.1 5,341,895 - -0.7 5,341,895 - -2.2 5,341,895 - -0.2 5,342,056 + +0.5 5,342,056 + -0.6 5,342,057 - +0.1 5,342,179 + +0.1 5,342,179 + +0.0 5,342,180 - +0.6 5,342,180 - -1.3 5,342,180 - -0.4 5,342,192 + +0.7 5,342,193 - -1.4 5,342,193 - +0.5 5,342,193 - +0.8 5,342,450 - +1.3 5,342,510 + Pf6N2E2_4617 0.11 -0.2 5,342,686 + Pf6N2E2_4617 0.14 -0.1 5,342,686 + Pf6N2E2_4617 0.14 +0.3 5,342,686 + Pf6N2E2_4617 0.14 -0.3 5,342,686 + Pf6N2E2_4617 0.14 -0.1 5,342,686 + Pf6N2E2_4617 0.14 +0.0 5,342,686 + Pf6N2E2_4617 0.14 -0.7 5,342,686 + Pf6N2E2_4617 0.14 -0.2 5,342,686 + Pf6N2E2_4617 0.14 -0.8 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,687 - Pf6N2E2_4617 0.14 -0.0 5,342,687 - Pf6N2E2_4617 0.14 +0.1 5,342,687 - Pf6N2E2_4617 0.14 +0.0 5,342,687 - Pf6N2E2_4617 0.14 -0.4 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,687 - Pf6N2E2_4617 0.14 +0.6 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,687 - Pf6N2E2_4617 0.14 -0.7 5,342,688 + Pf6N2E2_4617 0.14 +0.0 5,342,689 - Pf6N2E2_4617 0.14 -0.8 5,342,689 - Pf6N2E2_4617 0.14 -0.1 5,342,689 - Pf6N2E2_4617 0.14 -0.6 5,342,740 + Pf6N2E2_4617 0.15 +0.1 5,342,858 + Pf6N2E2_4617 0.17 -1.1 5,343,088 + Pf6N2E2_4617 0.20 -0.3 5,343,089 - Pf6N2E2_4617 0.20 -0.8 5,343,244 + Pf6N2E2_4617 0.23 -0.7 5,343,245 - Pf6N2E2_4617 0.23 -0.5 5,343,457 + Pf6N2E2_4617 0.27 -0.9 5,343,457 + Pf6N2E2_4617 0.27 -0.6 5,343,458 - Pf6N2E2_4617 0.27 -1.8 5,343,458 - Pf6N2E2_4617 0.27 -0.1 5,343,458 - Pf6N2E2_4617 0.27 -1.6 5,343,458 - Pf6N2E2_4617 0.27 -0.4 5,343,458 - Pf6N2E2_4617 0.27 -0.5 5,343,460 + Pf6N2E2_4617 0.27 -1.7 5,343,460 + Pf6N2E2_4617 0.27 +1.6 5,343,460 + Pf6N2E2_4617 0.27 -0.3 5,343,460 + Pf6N2E2_4617 0.27 -0.2 5,343,461 - Pf6N2E2_4617 0.27 -1.0 5,343,461 - Pf6N2E2_4617 0.27 +0.2 5,343,817 + Pf6N2E2_4617 0.33 -2.6 5,343,817 + Pf6N2E2_4617 0.33 -0.6 5,343,817 + Pf6N2E2_4617 0.33 -3.1 5,343,818 - Pf6N2E2_4617 0.33 -0.5 5,344,299 + Pf6N2E2_4617 0.41 -0.8 5,344,299 + Pf6N2E2_4617 0.41 -0.4 5,344,299 + Pf6N2E2_4617 0.41 -1.2 5,344,299 + Pf6N2E2_4617 0.41 +0.1 5,344,299 + Pf6N2E2_4617 0.41 -0.3 5,344,299 + Pf6N2E2_4617 0.41 -0.3 5,344,300 - Pf6N2E2_4617 0.41 -0.2 5,344,300 - Pf6N2E2_4617 0.41 +0.3 5,344,300 - Pf6N2E2_4617 0.41 -2.2 5,344,798 + Pf6N2E2_4617 0.49 -0.2 5,344,798 + Pf6N2E2_4617 0.49 +0.8 5,344,799 - Pf6N2E2_4617 0.49 +0.4 5,344,934 + Pf6N2E2_4617 0.52 +0.1 5,344,935 - Pf6N2E2_4617 0.52 -0.6 5,344,935 - Pf6N2E2_4617 0.52 -0.1 5,344,935 - Pf6N2E2_4617 0.52 -1.9 5,345,288 - Pf6N2E2_4617 0.57 -1.1 5,345,288 - +5.1 5,345,317 + Pf6N2E2_4617 0.58 +2.1 5,345,318 - Pf6N2E2_4617 0.58 -1.7 5,345,318 - Pf6N2E2_4617 0.58 +0.8 5,345,556 + Pf6N2E2_4617 0.62 -0.6 5,345,557 - Pf6N2E2_4617 0.62 -0.7 5,345,557 - Pf6N2E2_4617 0.62 -0.8 5,345,624 + Pf6N2E2_4617 0.63 -2.3 5,345,711 - Pf6N2E2_4617 0.65 -0.8 5,345,711 - Pf6N2E2_4617 0.65 -0.8 5,345,711 - Pf6N2E2_4617 0.65 -0.3 5,345,711 - Pf6N2E2_4617 0.65 -0.1 5,345,711 - Pf6N2E2_4617 0.65 -1.2 5,345,922 + Pf6N2E2_4617 0.68 -1.2 5,345,923 - Pf6N2E2_4617 0.68 -0.7 5,345,923 - Pf6N2E2_4617 0.68 -0.3 5,345,923 - Pf6N2E2_4617 0.68 +0.9 5,346,370 - Pf6N2E2_4617 0.76 -1.4 5,346,526 - Pf6N2E2_4617 0.78 -1.9 5,346,561 + Pf6N2E2_4617 0.79 -0.5 5,346,561 + Pf6N2E2_4617 0.79 -2.4 5,346,561 + Pf6N2E2_4617 0.79 +0.3 5,346,561 + +0.8 5,346,561 + Pf6N2E2_4617 0.79 -1.6 5,346,561 + Pf6N2E2_4617 0.79 -1.0 5,346,561 + Pf6N2E2_4617 0.79 +0.2 5,346,562 - Pf6N2E2_4617 0.79 -1.0 5,346,562 - Pf6N2E2_4617 0.79 +0.5 5,346,562 - Pf6N2E2_4617 0.79 +0.2 5,346,562 - Pf6N2E2_4617 0.79 -0.7 5,346,639 + Pf6N2E2_4617 0.80 -2.3 5,346,640 - Pf6N2E2_4617 0.80 -0.1 5,346,640 - Pf6N2E2_4617 0.80 -0.6 5,347,093 + Pf6N2E2_4617 0.88 -1.6 5,347,093 + Pf6N2E2_4617 0.88 +0.4 5,347,093 + Pf6N2E2_4617 0.88 -1.0 5,347,093 + Pf6N2E2_4617 0.88 +0.0 5,347,093 + Pf6N2E2_4617 0.88 -1.2 5,347,093 + Pf6N2E2_4617 0.88 -1.6 5,347,093 + Pf6N2E2_4617 0.88 -0.3 5,347,093 + Pf6N2E2_4617 0.88 +0.9 5,347,093 + Pf6N2E2_4617 0.88 -0.3 5,347,093 + Pf6N2E2_4617 0.88 -1.7 5,347,093 + Pf6N2E2_4617 0.88 -1.5 5,347,093 + Pf6N2E2_4617 0.88 -0.3 5,347,094 - Pf6N2E2_4617 0.88 -0.3 5,347,094 - Pf6N2E2_4617 0.88 -0.5 5,347,094 - Pf6N2E2_4617 0.88 -1.5 5,347,094 - Pf6N2E2_4617 0.88 -1.1 5,347,094 - Pf6N2E2_4617 0.88 -0.6 5,347,094 - Pf6N2E2_4617 0.88 +0.0 5,347,094 - Pf6N2E2_4617 0.88 -0.6 5,347,100 - Pf6N2E2_4617 0.88 -2.6 5,347,100 - Pf6N2E2_4617 0.88 -0.7 5,347,100 - Pf6N2E2_4617 0.88 -0.4 5,347,127 - Pf6N2E2_4617 0.88 +0.1 5,347,413 - -0.1 5,347,413 - -1.3 5,347,738 + -0.4 5,347,739 - -1.3 5,347,753 + +0.2 5,348,123 + Pf6N2E2_4618 0.67 +0.0 5,348,124 - Pf6N2E2_4618 0.67 -1.4 5,348,148 - Pf6N2E2_4618 0.72 -0.6 5,348,148 - Pf6N2E2_4618 0.72 +1.6 5,348,205 + Pf6N2E2_4618 0.84 -0.6 5,348,205 + Pf6N2E2_4618 0.84 +0.1 5,348,206 - Pf6N2E2_4618 0.84 -0.5 5,348,206 - Pf6N2E2_4618 0.84 -0.2 5,348,286 + -0.2 5,348,286 + -0.4 5,348,286 + +0.1 5,348,286 + +0.1 5,348,286 + +0.1 5,348,286 + -1.0 5,348,286 + +0.2 5,348,286 + -0.3 5,348,287 - +0.0 5,348,287 - -0.5 5,348,287 - +0.1 5,348,287 - +0.0 5,348,287 - +0.9 5,348,296 + -1.5 5,348,296 + -0.1 5,348,296 + +0.0 5,348,296 + +0.2 5,348,297 - -0.7 5,348,370 - -0.1 5,348,370 - +0.2 5,348,414 + -0.1 5,348,564 - +0.3 5,348,626 + -0.2 5,348,626 + -0.4 5,348,628 + +0.1 5,348,655 + +0.5 5,348,655 + -0.2 5,348,655 + -1.0 5,348,655 + +0.5 5,348,655 + -0.2 5,348,656 - -0.2 5,348,656 - -0.0 5,348,656 - +0.2 5,348,717 + -0.1 5,348,719 + -0.3 5,348,720 - -0.4
Or see this region's nucleotide sequence