Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4606 and Pf6N2E2_4607 are separated by 24 nucleotides Pf6N2E2_4607 and Pf6N2E2_4608 are separated by 85 nucleotides Pf6N2E2_4608 and Pf6N2E2_4609 overlap by 1 nucleotides
Pf6N2E2_4606 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 5,331,787 to 5,332,791
_4606
Pf6N2E2_4607 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 5,332,816 to 5,333,322
_4607
Pf6N2E2_4608 - Putative Holliday junction resolvase YggF, at 5,333,408 to 5,333,845
_4608
Pf6N2E2_4609 - UPF0301 protein YqgE, at 5,333,845 to 5,334,414
_4609
Position (kb)
5332
5333
5334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.818 kb on - strand at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.136 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.368 kb on - strand at 5333.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 5,332,806 - +0.8 5,332,806 - -0.2 5,332,806 - -1.4 5,332,806 - +0.6 5,332,806 - +0.1 5,332,806 - -0.3 5,332,806 - +0.8 5,332,818 - +0.4 5,333,036 - Pf6N2E2_4607 0.43 -1.5 5,333,036 - Pf6N2E2_4607 0.43 +0.0 5,333,036 - Pf6N2E2_4607 0.43 -3.1 5,333,036 - Pf6N2E2_4607 0.43 -1.4 5,333,036 - Pf6N2E2_4607 0.43 -1.2 5,333,136 - Pf6N2E2_4607 0.63 +0.1 5,333,232 - Pf6N2E2_4607 0.82 +0.4 5,333,232 - Pf6N2E2_4607 0.82 +0.1 5,333,232 - Pf6N2E2_4607 0.82 +2.5 5,333,368 - -0.7 5,333,374 - +1.2
Or see this region's nucleotide sequence