Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4119 and Pf6N2E2_4120 are separated by 127 nucleotides Pf6N2E2_4120 and Pf6N2E2_4121 are separated by 24 nucleotides
Pf6N2E2_4119 - Chaperone protein YscY (Yop proteins translocation protein Y), at 4,798,387 to 4,798,698
_4119
Pf6N2E2_4120 - Trk system potassium uptake protein TrkA, at 4,798,826 to 4,800,199
_4120
Pf6N2E2_4121 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 4,800,224 to 4,801,534
_4121
Position (kb)
4798
4799
4800
4801 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4797.865 kb on - strand at 4797.865 kb on - strand at 4797.865 kb on - strand at 4797.958 kb on + strand at 4797.987 kb on + strand at 4797.987 kb on + strand at 4797.987 kb on + strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.989 kb on + strand at 4797.989 kb on + strand at 4797.989 kb on + strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4798.023 kb on - strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.041 kb on - strand at 4798.041 kb on - strand at 4798.041 kb on - strand at 4798.130 kb on + strand at 4798.131 kb on - strand at 4798.163 kb on - strand at 4798.185 kb on + strand at 4798.244 kb on + strand at 4798.251 kb on + strand at 4798.251 kb on + strand at 4798.252 kb on - strand at 4798.252 kb on - strand at 4798.265 kb on - strand at 4798.265 kb on - strand at 4798.270 kb on + strand at 4798.270 kb on + strand at 4798.270 kb on + strand at 4798.271 kb on - strand at 4798.271 kb on - strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.275 kb on - strand at 4798.275 kb on - strand at 4798.275 kb on - strand at 4798.278 kb on + strand at 4798.278 kb on + strand at 4798.278 kb on + strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.284 kb on + strand at 4798.284 kb on + strand at 4798.284 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.289 kb on - strand at 4798.289 kb on - strand at 4798.289 kb on - strand at 4798.294 kb on + strand at 4798.294 kb on + strand at 4798.295 kb on - strand at 4798.332 kb on + strand at 4798.501 kb on + strand, within Pf6N2E2_4119 at 4798.501 kb on + strand, within Pf6N2E2_4119 at 4798.502 kb on - strand, within Pf6N2E2_4119 at 4798.534 kb on + strand, within Pf6N2E2_4119 at 4798.535 kb on - strand, within Pf6N2E2_4119 at 4798.553 kb on + strand at 4798.553 kb on + strand, within Pf6N2E2_4119 at 4798.553 kb on + strand, within Pf6N2E2_4119 at 4798.554 kb on - strand, within Pf6N2E2_4119 at 4798.554 kb on - strand, within Pf6N2E2_4119 at 4798.622 kb on + strand, within Pf6N2E2_4119 at 4798.633 kb on + strand, within Pf6N2E2_4119 at 4798.656 kb on + strand, within Pf6N2E2_4119 at 4799.398 kb on - strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.552 kb on - strand, within Pf6N2E2_4120 at 4799.719 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4800.261 kb on + strand at 4800.262 kb on - strand at 4800.262 kb on - strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.436 kb on + strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.933 kb on + strand, within Pf6N2E2_4121 at 4800.934 kb on - strand, within Pf6N2E2_4121 at 4800.982 kb on - strand, within Pf6N2E2_4121
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 4,797,865 - -0.0 4,797,865 - -1.0 4,797,865 - -0.5 4,797,958 + -0.5 4,797,987 + +0.1 4,797,987 + -0.5 4,797,987 + +0.0 4,797,988 - +0.8 4,797,988 - -0.2 4,797,988 - +1.5 4,797,988 - +0.3 4,797,988 - -0.6 4,797,988 - +1.2 4,797,989 + +3.3 4,797,989 + -0.3 4,797,989 + +1.0 4,797,990 - +0.0 4,797,990 - +0.2 4,797,990 - +0.0 4,797,990 - +0.5 4,797,990 - +0.5 4,797,990 - +0.1 4,797,990 - +0.0 4,797,990 - +0.1 4,797,990 - +0.6 4,797,990 - +0.0 4,798,023 - +0.8 4,798,040 + +0.3 4,798,040 + -0.6 4,798,040 + -1.3 4,798,040 + +0.1 4,798,041 - -0.8 4,798,041 - -1.0 4,798,041 - +0.0 4,798,130 + -1.5 4,798,131 - -0.7 4,798,163 - +0.3 4,798,185 + +0.7 4,798,244 + +0.4 4,798,251 + +0.4 4,798,251 + +0.8 4,798,252 - -0.4 4,798,252 - +0.4 4,798,265 - +0.4 4,798,265 - -0.3 4,798,270 + +0.2 4,798,270 + -0.5 4,798,270 + -0.6 4,798,271 - -0.4 4,798,271 - -0.8 4,798,274 + -1.0 4,798,274 + +0.2 4,798,274 + +0.3 4,798,274 + +0.2 4,798,274 + +1.3 4,798,275 - -0.6 4,798,275 - -0.1 4,798,275 - +0.3 4,798,278 + +1.3 4,798,278 + -0.7 4,798,278 + -0.2 4,798,279 - -0.1 4,798,279 - +0.5 4,798,279 - -0.0 4,798,279 - +0.7 4,798,279 - +1.0 4,798,279 - -0.2 4,798,279 - -0.7 4,798,279 - +0.3 4,798,279 - -0.0 4,798,279 - -0.0 4,798,279 - -0.7 4,798,279 - +1.1 4,798,279 - +1.3 4,798,279 - +0.2 4,798,284 + +0.5 4,798,284 + -0.9 4,798,284 + +0.6 4,798,288 + +1.0 4,798,288 + -0.3 4,798,288 + +0.2 4,798,288 + -1.4 4,798,289 - +1.0 4,798,289 - +0.6 4,798,289 - -0.6 4,798,294 + +0.6 4,798,294 + -0.3 4,798,295 - -0.1 4,798,332 + +0.1 4,798,501 + Pf6N2E2_4119 0.37 -0.3 4,798,501 + Pf6N2E2_4119 0.37 -0.1 4,798,502 - Pf6N2E2_4119 0.37 +0.3 4,798,534 + Pf6N2E2_4119 0.47 -0.7 4,798,535 - Pf6N2E2_4119 0.47 -0.8 4,798,553 + +1.9 4,798,553 + Pf6N2E2_4119 0.53 +0.1 4,798,553 + Pf6N2E2_4119 0.53 +0.6 4,798,554 - Pf6N2E2_4119 0.54 +0.1 4,798,554 - Pf6N2E2_4119 0.54 +1.1 4,798,622 + Pf6N2E2_4119 0.75 +1.0 4,798,633 + Pf6N2E2_4119 0.79 +0.3 4,798,656 + Pf6N2E2_4119 0.86 +0.6 4,799,398 - Pf6N2E2_4120 0.42 -1.0 4,799,508 + Pf6N2E2_4120 0.50 -1.6 4,799,508 + Pf6N2E2_4120 0.50 -1.3 4,799,508 + Pf6N2E2_4120 0.50 -1.5 4,799,508 + Pf6N2E2_4120 0.50 -2.8 4,799,508 + Pf6N2E2_4120 0.50 -1.8 4,799,508 + Pf6N2E2_4120 0.50 +0.2 4,799,509 - Pf6N2E2_4120 0.50 -0.7 4,799,509 - Pf6N2E2_4120 0.50 +0.5 4,799,509 - Pf6N2E2_4120 0.50 -1.4 4,799,509 - Pf6N2E2_4120 0.50 -0.1 4,799,509 - Pf6N2E2_4120 0.50 -1.4 4,799,509 - Pf6N2E2_4120 0.50 -0.8 4,799,509 - Pf6N2E2_4120 0.50 -1.0 4,799,509 - Pf6N2E2_4120 0.50 -1.3 4,799,509 - Pf6N2E2_4120 0.50 -0.6 4,799,509 - Pf6N2E2_4120 0.50 -0.7 4,799,552 - Pf6N2E2_4120 0.53 -1.5 4,799,719 - Pf6N2E2_4120 0.65 -1.7 4,799,722 - Pf6N2E2_4120 0.65 -0.3 4,799,722 - Pf6N2E2_4120 0.65 +0.1 4,799,722 - Pf6N2E2_4120 0.65 -0.7 4,799,806 + Pf6N2E2_4120 0.71 -1.0 4,799,806 + Pf6N2E2_4120 0.71 -1.2 4,799,806 + Pf6N2E2_4120 0.71 -0.3 4,799,806 + Pf6N2E2_4120 0.71 -1.0 4,799,807 - Pf6N2E2_4120 0.71 -0.6 4,799,807 - Pf6N2E2_4120 0.71 -1.5 4,799,807 - Pf6N2E2_4120 0.71 -1.1 4,799,807 - Pf6N2E2_4120 0.71 -0.3 4,799,807 - Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -2.4 4,799,808 + Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -1.6 4,799,808 + Pf6N2E2_4120 0.71 -3.5 4,799,808 + Pf6N2E2_4120 0.71 -0.5 4,799,808 + Pf6N2E2_4120 0.71 -0.2 4,799,808 + Pf6N2E2_4120 0.71 -0.7 4,799,808 + Pf6N2E2_4120 0.71 -1.1 4,799,808 + Pf6N2E2_4120 0.71 +0.8 4,799,808 + Pf6N2E2_4120 0.71 -2.4 4,799,808 + Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -1.2 4,799,808 + Pf6N2E2_4120 0.71 -0.8 4,799,808 + Pf6N2E2_4120 0.71 -1.8 4,799,808 + Pf6N2E2_4120 0.71 -1.8 4,799,808 + Pf6N2E2_4120 0.71 -0.7 4,799,808 + Pf6N2E2_4120 0.71 -3.1 4,799,808 + Pf6N2E2_4120 0.71 -1.9 4,799,809 - Pf6N2E2_4120 0.72 -0.6 4,799,809 - Pf6N2E2_4120 0.72 -0.1 4,799,809 - Pf6N2E2_4120 0.72 -0.7 4,799,809 - Pf6N2E2_4120 0.72 +0.7 4,799,809 - Pf6N2E2_4120 0.72 +0.0 4,799,809 - Pf6N2E2_4120 0.72 -0.5 4,799,809 - Pf6N2E2_4120 0.72 -1.6 4,799,809 - Pf6N2E2_4120 0.72 -0.4 4,799,809 - Pf6N2E2_4120 0.72 -2.2 4,799,809 - Pf6N2E2_4120 0.72 -2.6 4,799,809 - Pf6N2E2_4120 0.72 -1.9 4,799,809 - Pf6N2E2_4120 0.72 -0.9 4,799,809 - Pf6N2E2_4120 0.72 -1.2 4,799,809 - Pf6N2E2_4120 0.72 -2.5 4,799,809 - Pf6N2E2_4120 0.72 +0.5 4,799,809 - Pf6N2E2_4120 0.72 -2.2 4,799,809 - Pf6N2E2_4120 0.72 -1.5 4,799,809 - Pf6N2E2_4120 0.72 -0.2 4,800,261 + -0.4 4,800,262 - +0.7 4,800,262 - +0.0 4,800,264 + +0.5 4,800,264 + -0.7 4,800,264 + -1.6 4,800,264 + -0.4 4,800,265 - +1.1 4,800,265 - +0.0 4,800,265 - +0.4 4,800,265 - -0.3 4,800,436 + Pf6N2E2_4121 0.16 -1.2 4,800,437 - Pf6N2E2_4121 0.16 -0.3 4,800,437 - Pf6N2E2_4121 0.16 -0.1 4,800,437 - Pf6N2E2_4121 0.16 +0.7 4,800,437 - Pf6N2E2_4121 0.16 +0.3 4,800,933 + Pf6N2E2_4121 0.54 +0.1 4,800,934 - Pf6N2E2_4121 0.54 +0.5 4,800,982 - Pf6N2E2_4121 0.58 +0.3
Or see this region's nucleotide sequence