Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2720 and Pf6N2E2_2721 are separated by 77 nucleotides Pf6N2E2_2721 and Pf6N2E2_2722 are separated by 102 nucleotides Pf6N2E2_2722 and Pf6N2E2_2723 are separated by 52 nucleotides Pf6N2E2_2723 and Pf6N2E2_2724 are separated by 160 nucleotides
Pf6N2E2_2720 - tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34), at 3,213,049 to 3,213,519
_2720
Pf6N2E2_2721 - FIG00954302: hypothetical protein, at 3,213,597 to 3,213,974
_2721
Pf6N2E2_2722 - FIG00954807: hypothetical protein, at 3,214,077 to 3,215,285
_2722
Pf6N2E2_2723 - YcgN (Fragment), at 3,215,338 to 3,215,787
_2723
Pf6N2E2_2724 - Nitroreductase family protein, at 3,215,948 to 3,216,541
_2724
Position (kb)
3214
3215
3216 Strain fitness (log2 ratio)
-1
0
1 at 3213.136 kb on + strand, within Pf6N2E2_2720 at 3213.157 kb on + strand, within Pf6N2E2_2720 at 3213.157 kb on + strand, within Pf6N2E2_2720 at 3213.158 kb on - strand, within Pf6N2E2_2720 at 3213.158 kb on - strand, within Pf6N2E2_2720 at 3213.172 kb on + strand, within Pf6N2E2_2720 at 3213.173 kb on - strand, within Pf6N2E2_2720 at 3213.217 kb on + strand, within Pf6N2E2_2720 at 3213.217 kb on + strand, within Pf6N2E2_2720 at 3213.218 kb on - strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.466 kb on + strand, within Pf6N2E2_2720 at 3213.466 kb on + strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.569 kb on - strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.571 kb on - strand at 3213.571 kb on - strand at 3213.571 kb on - strand at 3213.582 kb on + strand at 3213.582 kb on + strand at 3213.583 kb on - strand at 3213.605 kb on + strand at 3214.106 kb on + strand at 3214.106 kb on + strand at 3214.107 kb on - strand at 3214.352 kb on - strand, within Pf6N2E2_2722 at 3214.399 kb on + strand, within Pf6N2E2_2722 at 3214.399 kb on + strand, within Pf6N2E2_2722 at 3214.400 kb on - strand, within Pf6N2E2_2722 at 3214.531 kb on + strand, within Pf6N2E2_2722 at 3214.531 kb on + strand, within Pf6N2E2_2722 at 3214.532 kb on - strand, within Pf6N2E2_2722 at 3214.626 kb on + strand, within Pf6N2E2_2722 at 3214.626 kb on + strand, within Pf6N2E2_2722 at 3215.403 kb on + strand, within Pf6N2E2_2723 at 3215.403 kb on + strand, within Pf6N2E2_2723 at 3215.403 kb on + strand, within Pf6N2E2_2723 at 3215.403 kb on + strand, within Pf6N2E2_2723 at 3215.404 kb on - strand, within Pf6N2E2_2723 at 3215.404 kb on - strand, within Pf6N2E2_2723 at 3215.649 kb on - strand, within Pf6N2E2_2723 at 3215.649 kb on - strand, within Pf6N2E2_2723 at 3215.649 kb on - strand, within Pf6N2E2_2723 at 3215.649 kb on - strand, within Pf6N2E2_2723 at 3215.830 kb on - strand at 3215.830 kb on - strand at 3215.905 kb on + strand at 3215.905 kb on + strand at 3215.905 kb on + strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.941 kb on + strand at 3215.941 kb on + strand at 3215.942 kb on - strand at 3216.092 kb on + strand, within Pf6N2E2_2724 at 3216.119 kb on + strand, within Pf6N2E2_2724 at 3216.119 kb on + strand, within Pf6N2E2_2724
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 3,213,136 + Pf6N2E2_2720 0.18 -0.3 3,213,157 + Pf6N2E2_2720 0.23 +0.5 3,213,157 + Pf6N2E2_2720 0.23 -0.2 3,213,158 - Pf6N2E2_2720 0.23 +0.4 3,213,158 - Pf6N2E2_2720 0.23 -0.3 3,213,172 + Pf6N2E2_2720 0.26 -1.1 3,213,173 - Pf6N2E2_2720 0.26 +0.1 3,213,217 + Pf6N2E2_2720 0.36 -0.5 3,213,217 + Pf6N2E2_2720 0.36 +0.1 3,213,218 - Pf6N2E2_2720 0.36 -1.6 3,213,322 + Pf6N2E2_2720 0.58 +1.2 3,213,322 + Pf6N2E2_2720 0.58 -0.3 3,213,322 + Pf6N2E2_2720 0.58 +1.4 3,213,323 - Pf6N2E2_2720 0.58 +0.2 3,213,323 - Pf6N2E2_2720 0.58 -0.2 3,213,323 - Pf6N2E2_2720 0.58 +0.2 3,213,412 + Pf6N2E2_2720 0.77 -0.6 3,213,412 + Pf6N2E2_2720 0.77 +0.4 3,213,412 + Pf6N2E2_2720 0.77 +0.8 3,213,412 + Pf6N2E2_2720 0.77 +0.3 3,213,412 + Pf6N2E2_2720 0.77 +0.4 3,213,412 + Pf6N2E2_2720 0.77 -0.2 3,213,413 - Pf6N2E2_2720 0.77 +0.7 3,213,413 - Pf6N2E2_2720 0.77 -0.1 3,213,413 - Pf6N2E2_2720 0.77 +0.6 3,213,413 - Pf6N2E2_2720 0.77 -0.6 3,213,413 - Pf6N2E2_2720 0.77 +1.6 3,213,413 - Pf6N2E2_2720 0.77 +0.3 3,213,413 - Pf6N2E2_2720 0.77 -0.7 3,213,413 - Pf6N2E2_2720 0.77 +0.8 3,213,466 + Pf6N2E2_2720 0.89 +0.5 3,213,466 + Pf6N2E2_2720 0.89 +0.4 3,213,467 - Pf6N2E2_2720 0.89 +0.1 3,213,467 - Pf6N2E2_2720 0.89 -1.2 3,213,467 - Pf6N2E2_2720 0.89 -0.1 3,213,568 + -0.4 3,213,568 + +0.5 3,213,568 + -0.5 3,213,568 + +0.8 3,213,568 + -0.2 3,213,568 + -0.3 3,213,569 - -0.7 3,213,570 + +0.1 3,213,570 + +0.8 3,213,570 + -0.7 3,213,570 + +0.0 3,213,570 + +0.6 3,213,571 - -0.5 3,213,571 - -0.1 3,213,571 - +0.1 3,213,582 + -0.0 3,213,582 + +0.5 3,213,583 - +0.3 3,213,605 + -0.3 3,214,106 + -0.3 3,214,106 + +0.5 3,214,107 - -0.2 3,214,352 - Pf6N2E2_2722 0.23 -0.9 3,214,399 + Pf6N2E2_2722 0.27 -0.4 3,214,399 + Pf6N2E2_2722 0.27 -1.3 3,214,400 - Pf6N2E2_2722 0.27 -0.2 3,214,531 + Pf6N2E2_2722 0.38 -0.2 3,214,531 + Pf6N2E2_2722 0.38 -0.1 3,214,532 - Pf6N2E2_2722 0.38 +0.4 3,214,626 + Pf6N2E2_2722 0.45 -1.2 3,214,626 + Pf6N2E2_2722 0.45 -0.3 3,215,403 + Pf6N2E2_2723 0.14 +0.2 3,215,403 + Pf6N2E2_2723 0.14 -0.1 3,215,403 + Pf6N2E2_2723 0.14 +0.4 3,215,403 + Pf6N2E2_2723 0.14 -0.7 3,215,404 - Pf6N2E2_2723 0.15 -0.5 3,215,404 - Pf6N2E2_2723 0.15 -0.2 3,215,649 - Pf6N2E2_2723 0.69 +0.1 3,215,649 - Pf6N2E2_2723 0.69 -0.5 3,215,649 - Pf6N2E2_2723 0.69 +0.4 3,215,649 - Pf6N2E2_2723 0.69 +0.2 3,215,830 - +0.9 3,215,830 - +0.9 3,215,905 + +0.4 3,215,905 + +0.9 3,215,905 + -0.3 3,215,906 - -0.2 3,215,906 - -0.1 3,215,906 - -0.3 3,215,906 - +0.4 3,215,906 - -0.1 3,215,941 + -0.3 3,215,941 + -0.7 3,215,942 - -0.0 3,216,092 + Pf6N2E2_2724 0.24 +0.0 3,216,119 + Pf6N2E2_2724 0.29 +0.3 3,216,119 + Pf6N2E2_2724 0.29 -0.3
Or see this region's nucleotide sequence