Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2706 and Pf6N2E2_2707 overlap by 4 nucleotides Pf6N2E2_2707 and Pf6N2E2_2708 are separated by 8 nucleotides Pf6N2E2_2708 and Pf6N2E2_2709 are separated by 64 nucleotides
Pf6N2E2_2706 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 3,199,240 to 3,200,160
_2706
Pf6N2E2_2707 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,200,157 to 3,200,849
_2707
Pf6N2E2_2708 - Lactam utilization protein LamB, at 3,200,858 to 3,201,610
_2708
Pf6N2E2_2709 - Sialic acid transporter (permease) NanT, at 3,201,675 to 3,202,961
_2709
Position (kb)
3200
3201 Strain fitness (log2 ratio)
-2
-1
0
1 at 3199.316 kb on - strand at 3199.493 kb on + strand, within Pf6N2E2_2706 at 3199.494 kb on - strand, within Pf6N2E2_2706 at 3199.694 kb on + strand, within Pf6N2E2_2706 at 3199.695 kb on - strand, within Pf6N2E2_2706 at 3199.975 kb on - strand, within Pf6N2E2_2706 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.576 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.709 kb on - strand, within Pf6N2E2_2707 at 3200.710 kb on + strand, within Pf6N2E2_2707 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3201.090 kb on + strand, within Pf6N2E2_2708 at 3201.091 kb on - strand, within Pf6N2E2_2708 at 3201.204 kb on + strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.288 kb on + strand, within Pf6N2E2_2708 at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.643 kb on - strand at 3201.643 kb on - strand at 3201.676 kb on + strand at 3201.676 kb on + strand at 3201.743 kb on - strand at 3201.743 kb on - strand at 3201.847 kb on + strand, within Pf6N2E2_2709
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 3,199,316 - -0.1 3,199,493 + Pf6N2E2_2706 0.27 +0.1 3,199,494 - Pf6N2E2_2706 0.28 -0.3 3,199,694 + Pf6N2E2_2706 0.49 +0.7 3,199,695 - Pf6N2E2_2706 0.49 -0.5 3,199,975 - Pf6N2E2_2706 0.80 -0.8 3,200,483 - Pf6N2E2_2707 0.47 +0.1 3,200,483 - Pf6N2E2_2707 0.47 -1.1 3,200,576 - Pf6N2E2_2707 0.60 -0.6 3,200,690 - Pf6N2E2_2707 0.77 -0.5 3,200,690 - Pf6N2E2_2707 0.77 -0.9 3,200,709 - Pf6N2E2_2707 0.80 -1.8 3,200,710 + Pf6N2E2_2707 0.80 -0.1 3,200,954 - Pf6N2E2_2708 0.13 -0.3 3,200,954 - Pf6N2E2_2708 0.13 +0.1 3,200,954 - Pf6N2E2_2708 0.13 -0.8 3,201,090 + Pf6N2E2_2708 0.31 -0.5 3,201,091 - Pf6N2E2_2708 0.31 -1.0 3,201,204 + Pf6N2E2_2708 0.46 -0.7 3,201,265 - Pf6N2E2_2708 0.54 -1.2 3,201,265 - Pf6N2E2_2708 0.54 +0.8 3,201,265 - Pf6N2E2_2708 0.54 -1.7 3,201,265 - Pf6N2E2_2708 0.54 -1.3 3,201,265 - Pf6N2E2_2708 0.54 -0.7 3,201,288 + Pf6N2E2_2708 0.57 -0.6 3,201,626 - +0.5 3,201,626 - +0.2 3,201,626 - +0.7 3,201,626 - +0.4 3,201,643 - -0.5 3,201,643 - -1.0 3,201,676 + +0.5 3,201,676 + +0.6 3,201,743 - +0.4 3,201,743 - -0.4 3,201,847 + Pf6N2E2_2709 0.13 -0.2
Or see this region's nucleotide sequence