Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2541

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
2,999,334	+		Pf6N2E2_2540	0.46	+0.2
2,999,334	+		Pf6N2E2_2540	0.46	+0.8
2,999,344	+		Pf6N2E2_2540	0.47	+0.1
2,999,502	+		Pf6N2E2_2540	0.57	-1.3
2,999,577	+		Pf6N2E2_2540	0.62	-0.1
2,999,577	+		Pf6N2E2_2540	0.62	-0.3
2,999,577	+		Pf6N2E2_2540	0.62	-0.4
2,999,578	-		Pf6N2E2_2540	0.62	+0.3
2,999,578	-		Pf6N2E2_2540	0.62	-0.2
2,999,578	-		Pf6N2E2_2540	0.62	+0.6
2,999,578	-		Pf6N2E2_2540	0.62	+0.5
2,999,595	+		Pf6N2E2_2540	0.63	-2.1
2,999,595	+		Pf6N2E2_2540	0.63	-0.3
2,999,595	+		Pf6N2E2_2540	0.63	-0.0
2,999,595	+		Pf6N2E2_2540	0.63	+1.4
2,999,595	+		Pf6N2E2_2540	0.63	-1.5
2,999,595	+		Pf6N2E2_2540	0.63	-0.7
2,999,596	-		Pf6N2E2_2540	0.63	+0.1
2,999,596	-		Pf6N2E2_2540	0.63	+1.3
2,999,596	-		Pf6N2E2_2540	0.63	-0.1
2,999,830	-		Pf6N2E2_2540	0.79	+0.3
2,999,830	-		Pf6N2E2_2540	0.79	+0.2
3,000,186	+				-0.2
3,000,186	+				-0.1
3,000,316	+				+0.7
3,000,316	+				+0.3
3,000,316	+				-0.2
3,000,317	-				-0.1
3,000,317	-				-0.1
3,000,317	-				+0.5
3,000,384	+				-0.9
3,000,384	+				+0.1
3,000,385	-				-0.6
3,000,385	-				+0.0
3,000,420	+				-1.0
3,000,420	+				-0.3
3,000,420	+				+0.7
3,000,421	-				+0.0
3,000,597	+		Pf6N2E2_2541	0.15	-1.0
3,000,597	+		Pf6N2E2_2541	0.15	-0.5
3,000,681	+		Pf6N2E2_2541	0.19	-1.4
3,000,681	+		Pf6N2E2_2541	0.19	-0.9
3,000,681	+		Pf6N2E2_2541	0.19	-0.9
3,000,682	-		Pf6N2E2_2541	0.19	+1.7
3,000,691	+		Pf6N2E2_2541	0.19	-0.4
3,000,691	+		Pf6N2E2_2541	0.19	-1.0
3,000,692	-		Pf6N2E2_2541	0.19	+0.5
3,001,006	+		Pf6N2E2_2541	0.35	-1.7
3,001,059	+		Pf6N2E2_2541	0.38	-1.2
3,001,060	-		Pf6N2E2_2541	0.38	-2.6
3,001,230	+		Pf6N2E2_2541	0.47	-0.1
3,001,230	+		Pf6N2E2_2541	0.47	+0.7
3,001,231	-		Pf6N2E2_2541	0.47	-0.6
3,001,718	+		Pf6N2E2_2541	0.71	+0.3
3,001,718	+		Pf6N2E2_2541	0.71	-0.6
3,001,719	-		Pf6N2E2_2541	0.71	-0.0
3,001,719	-		Pf6N2E2_2541	0.71	-0.7
3,001,794	+		Pf6N2E2_2541	0.75	+0.0
3,001,794	+		Pf6N2E2_2541	0.75	-0.2
3,001,794	+		Pf6N2E2_2541	0.75	-1.7
3,001,795	-		Pf6N2E2_2541	0.75	-1.3
3,001,795	-		Pf6N2E2_2541	0.75	-0.3
3,001,795	-		Pf6N2E2_2541	0.75	-2.1
3,001,795	-		Pf6N2E2_2541	0.75	-1.1
3,002,004	+		Pf6N2E2_2541	0.86	+0.3
3,002,004	+		Pf6N2E2_2541	0.86	-0.3
3,002,005	-		Pf6N2E2_2541	0.86	-0.6
3,002,005	-		Pf6N2E2_2541	0.86	-1.1
3,002,005	-		Pf6N2E2_2541	0.86	+0.6
3,002,005	-		Pf6N2E2_2541	0.86	-1.5
3,002,121	+				-1.3
3,002,121	+				-0.7
3,002,121	+				-0.7
3,002,122	-				-0.3
3,002,122	-				-0.3
3,002,122	-				-0.2
3,002,122	-				-0.5
3,002,296	+				+0.1
3,002,296	+				-0.3
3,002,297	-				-0.5
3,002,297	-				-0.6
3,002,454	+				+0.0
3,002,455	-				+0.0
3,002,470	-				-0.5
3,002,565	+				+0.1
3,002,565	+				-0.9
3,002,565	+				+0.2
3,002,565	+				-1.1
3,002,565	+				+0.1
3,002,565	+				+0.4
3,002,565	+				-1.5
3,002,565	+				-0.2
3,002,565	+				+0.3
3,002,566	-				-0.9
3,002,566	-				+0.3
3,002,566	-				+0.5
3,002,566	-				+0.3
3,002,566	-				-1.1
3,002,566	-				-0.0
3,002,577	-				+0.3
3,002,577	-				+0.5
3,002,577	-				+0.1
3,002,577	-				+0.1
3,002,588	+				-0.5
3,002,588	+				-0.5
3,002,588	+				+0.6
3,002,588	+				+0.6
3,002,625	-				-0.6
3,002,800	+		Pf6N2E2_2542	0.13	-0.0
3,002,800	+		Pf6N2E2_2542	0.13	+0.7
3,002,800	+		Pf6N2E2_2542	0.13	-0.0
3,002,800	+		Pf6N2E2_2542	0.13	-0.6
3,002,801	-		Pf6N2E2_2542	0.13	+0.2
3,002,801	-		Pf6N2E2_2542	0.13	-0.2
3,002,801	-		Pf6N2E2_2542	0.13	+0.5
3,002,806	+		Pf6N2E2_2542	0.14	+0.1
3,002,806	+		Pf6N2E2_2542	0.14	+0.6
3,002,842	+		Pf6N2E2_2542	0.16	-1.0
3,002,851	+		Pf6N2E2_2542	0.16	+0.7
3,002,851	+		Pf6N2E2_2542	0.16	-0.3
3,002,852	-		Pf6N2E2_2542	0.16	+0.0
3,003,007	+		Pf6N2E2_2542	0.25	+0.0
3,003,007	+		Pf6N2E2_2542	0.25	-0.3
3,003,008	-		Pf6N2E2_2542	0.25	-0.8
3,003,008	-		Pf6N2E2_2542	0.25	-0.3
3,003,008	-		Pf6N2E2_2542	0.25	+0.0
3,003,032	+		Pf6N2E2_2542	0.26	-0.1
3,003,112	+		Pf6N2E2_2542	0.31	+0.7
3,003,113	-		Pf6N2E2_2542	0.31	+0.2
3,003,113	-		Pf6N2E2_2542	0.31	+0.7
3,003,245	-		Pf6N2E2_2542	0.38	+0.2