Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_222 and Pf6N2E2_223 overlap by 4 nucleotides Pf6N2E2_223 and Pf6N2E2_224 are separated by 242 nucleotides
Pf6N2E2_222 - RND efflux system, inner membrane transporter CmeB, at 280,435 to 283,581
_222
Pf6N2E2_223 - RND efflux system, outer membrane lipoprotein CmeC, at 283,578 to 284,975
_223
Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216
_224
Position (kb) 283
284
285 Strain fitness (log2 ratio) -1
0
1 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.742 kb on + strand, within Pf6N2E2_222 at 282.742 kb on + strand, within Pf6N2E2_222 at 282.743 kb on - strand, within Pf6N2E2_222 at 282.743 kb on - strand, within Pf6N2E2_222 at 282.859 kb on + strand, within Pf6N2E2_222 at 282.860 kb on - strand, within Pf6N2E2_222 at 282.884 kb on - strand, within Pf6N2E2_222 at 283.018 kb on + strand, within Pf6N2E2_222 at 283.019 kb on - strand, within Pf6N2E2_222 at 283.057 kb on + strand, within Pf6N2E2_222 at 283.080 kb on - strand, within Pf6N2E2_222 at 283.204 kb on + strand, within Pf6N2E2_222 at 283.382 kb on + strand at 283.650 kb on + strand at 283.650 kb on + strand at 283.651 kb on - strand at 283.651 kb on - strand at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.986 kb on + strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 284.043 kb on + strand, within Pf6N2E2_223 at 284.044 kb on - strand, within Pf6N2E2_223 at 284.181 kb on + strand, within Pf6N2E2_223 at 284.181 kb on + strand, within Pf6N2E2_223 at 284.249 kb on + strand, within Pf6N2E2_223 at 284.367 kb on + strand, within Pf6N2E2_223 at 284.691 kb on + strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.850 kb on + strand at 284.883 kb on + strand at 284.884 kb on - strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.947 kb on - strand at 284.947 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.994 kb on + strand at 284.995 kb on - strand at 285.189 kb on - strand at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.697 kb on - strand, within Pf6N2E2_224 at 285.748 kb on + strand, within Pf6N2E2_224 at 285.952 kb on + strand, within Pf6N2E2_224
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 282,700 + Pf6N2E2_222 0.72 +0.3 282,700 + Pf6N2E2_222 0.72 +0.0 282,700 + Pf6N2E2_222 0.72 +0.9 282,700 + Pf6N2E2_222 0.72 -0.9 282,700 + Pf6N2E2_222 0.72 -0.3 282,700 + Pf6N2E2_222 0.72 +0.1 282,700 + Pf6N2E2_222 0.72 -1.1 282,700 + Pf6N2E2_222 0.72 +0.4 282,700 + Pf6N2E2_222 0.72 +0.1 282,700 + Pf6N2E2_222 0.72 -0.6 282,701 - Pf6N2E2_222 0.72 -0.4 282,701 - Pf6N2E2_222 0.72 +0.0 282,701 - Pf6N2E2_222 0.72 +0.8 282,701 - Pf6N2E2_222 0.72 +0.6 282,701 - Pf6N2E2_222 0.72 -1.2 282,742 + Pf6N2E2_222 0.73 +0.5 282,742 + Pf6N2E2_222 0.73 -0.3 282,743 - Pf6N2E2_222 0.73 +0.4 282,743 - Pf6N2E2_222 0.73 +0.6 282,859 + Pf6N2E2_222 0.77 -0.9 282,860 - Pf6N2E2_222 0.77 +0.3 282,884 - Pf6N2E2_222 0.78 +0.4 283,018 + Pf6N2E2_222 0.82 -0.5 283,019 - Pf6N2E2_222 0.82 +0.6 283,057 + Pf6N2E2_222 0.83 +0.1 283,080 - Pf6N2E2_222 0.84 +0.3 283,204 + Pf6N2E2_222 0.88 -1.0 283,382 + +0.0 283,650 + +0.1 283,650 + -0.1 283,651 - -0.3 283,651 - +0.2 283,959 + Pf6N2E2_223 0.27 -1.1 283,959 + Pf6N2E2_223 0.27 +0.1 283,959 + Pf6N2E2_223 0.27 +0.6 283,959 + Pf6N2E2_223 0.27 +0.2 283,986 + Pf6N2E2_223 0.29 -0.2 283,987 - Pf6N2E2_223 0.29 -0.1 283,987 - Pf6N2E2_223 0.29 +0.5 283,987 - Pf6N2E2_223 0.29 +0.4 283,987 - Pf6N2E2_223 0.29 +0.5 284,043 + Pf6N2E2_223 0.33 +0.1 284,044 - Pf6N2E2_223 0.33 -0.1 284,181 + Pf6N2E2_223 0.43 -0.3 284,181 + Pf6N2E2_223 0.43 -0.1 284,249 + Pf6N2E2_223 0.48 -0.3 284,367 + Pf6N2E2_223 0.56 +0.0 284,691 + Pf6N2E2_223 0.80 +0.3 284,692 - Pf6N2E2_223 0.80 +0.5 284,692 - Pf6N2E2_223 0.80 +1.1 284,850 + -0.4 284,883 + -0.4 284,884 - -0.8 284,946 + +0.7 284,946 + -0.1 284,946 + -0.1 284,947 - +0.8 284,947 - +0.2 284,974 - -0.1 284,974 - +0.4 284,974 - +0.4 284,994 + +0.4 284,995 - +0.2 285,189 - +0.6 285,423 + Pf6N2E2_224 0.21 +1.2 285,423 + Pf6N2E2_224 0.21 +0.4 285,423 + Pf6N2E2_224 0.21 -0.1 285,424 - Pf6N2E2_224 0.21 +0.3 285,424 - Pf6N2E2_224 0.21 +0.2 285,697 - Pf6N2E2_224 0.48 +1.0 285,748 + Pf6N2E2_224 0.53 +0.5 285,952 + Pf6N2E2_224 0.73 +0.7
Or see this region's nucleotide sequence