Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1617 and Pf6N2E2_1618 are separated by 25 nucleotides Pf6N2E2_1618 and Pf6N2E2_1619 are separated by 44 nucleotides Pf6N2E2_1619 and Pf6N2E2_1620 are separated by 25 nucleotides
Pf6N2E2_1617 - COG2199: FOG: GGDEF domain, at 1,890,640 to 1,891,980
_1617
Pf6N2E2_1618 - ABC-type amino acid transport, signal transduction systems, periplasmic component/domain, at 1,892,006 to 1,892,740
_1618
Pf6N2E2_1619 - Glutathione S-transferase (EC 2.5.1.18), at 1,892,785 to 1,893,624
_1619
Pf6N2E2_1620 - 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases, at 1,893,650 to 1,895,044
_1620
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-1
0
1
2 at 1891.860 kb on - strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.005 kb on + strand at 1892.005 kb on + strand at 1892.005 kb on + strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.041 kb on - strand at 1892.105 kb on + strand, within Pf6N2E2_1618 at 1892.106 kb on - strand, within Pf6N2E2_1618 at 1892.112 kb on - strand, within Pf6N2E2_1618 at 1892.246 kb on - strand, within Pf6N2E2_1618 at 1892.292 kb on + strand, within Pf6N2E2_1618 at 1892.293 kb on - strand, within Pf6N2E2_1618 at 1892.313 kb on + strand, within Pf6N2E2_1618 at 1892.337 kb on - strand, within Pf6N2E2_1618 at 1892.353 kb on + strand, within Pf6N2E2_1618 at 1892.354 kb on - strand, within Pf6N2E2_1618 at 1892.394 kb on - strand, within Pf6N2E2_1618 at 1892.394 kb on - strand, within Pf6N2E2_1618 at 1892.433 kb on - strand, within Pf6N2E2_1618 at 1892.597 kb on + strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.801 kb on - strand at 1892.926 kb on + strand, within Pf6N2E2_1619 at 1892.927 kb on - strand, within Pf6N2E2_1619 at 1892.995 kb on - strand, within Pf6N2E2_1619 at 1892.995 kb on - strand, within Pf6N2E2_1619 at 1893.278 kb on - strand, within Pf6N2E2_1619 at 1893.304 kb on + strand, within Pf6N2E2_1619 at 1893.397 kb on + strand, within Pf6N2E2_1619 at 1893.397 kb on + strand, within Pf6N2E2_1619 at 1893.431 kb on - strand, within Pf6N2E2_1619 at 1893.457 kb on + strand, within Pf6N2E2_1619 at 1893.490 kb on + strand, within Pf6N2E2_1619 at 1893.491 kb on - strand, within Pf6N2E2_1619 at 1893.491 kb on - strand, within Pf6N2E2_1619 at 1893.491 kb on - strand, within Pf6N2E2_1619 at 1893.732 kb on + strand at 1893.732 kb on + strand at 1893.733 kb on - strand at 1893.753 kb on + strand at 1893.781 kb on + strand at 1893.781 kb on + strand at 1893.782 kb on - strand at 1893.907 kb on - strand, within Pf6N2E2_1620 at 1893.907 kb on - strand, within Pf6N2E2_1620 at 1893.956 kb on + strand, within Pf6N2E2_1620 at 1893.996 kb on + strand, within Pf6N2E2_1620 at 1894.203 kb on - strand, within Pf6N2E2_1620 at 1894.617 kb on + strand, within Pf6N2E2_1620 at 1894.618 kb on - strand, within Pf6N2E2_1620
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,891,860 - +0.3 1,892,003 + +0.6 1,892,003 + +0.1 1,892,003 + +0.4 1,892,003 + -0.1 1,892,003 + +0.4 1,892,004 - +0.4 1,892,004 - +0.2 1,892,004 - +0.5 1,892,004 - -0.1 1,892,004 - +0.3 1,892,005 + +0.4 1,892,005 + -1.0 1,892,005 + +0.4 1,892,006 - +0.2 1,892,006 - -0.2 1,892,006 - +0.1 1,892,006 - +0.6 1,892,041 - -0.1 1,892,105 + Pf6N2E2_1618 0.13 +0.8 1,892,106 - Pf6N2E2_1618 0.14 +1.6 1,892,112 - Pf6N2E2_1618 0.14 -0.9 1,892,246 - Pf6N2E2_1618 0.33 +0.1 1,892,292 + Pf6N2E2_1618 0.39 +0.7 1,892,293 - Pf6N2E2_1618 0.39 -0.3 1,892,313 + Pf6N2E2_1618 0.42 +0.8 1,892,337 - Pf6N2E2_1618 0.45 -0.7 1,892,353 + Pf6N2E2_1618 0.47 +0.1 1,892,354 - Pf6N2E2_1618 0.47 -0.9 1,892,394 - Pf6N2E2_1618 0.53 +2.0 1,892,394 - Pf6N2E2_1618 0.53 -0.8 1,892,433 - Pf6N2E2_1618 0.58 -0.9 1,892,597 + Pf6N2E2_1618 0.80 +0.1 1,892,598 - Pf6N2E2_1618 0.81 +0.6 1,892,598 - Pf6N2E2_1618 0.81 -0.7 1,892,598 - Pf6N2E2_1618 0.81 +0.5 1,892,598 - Pf6N2E2_1618 0.81 +0.1 1,892,801 - -0.4 1,892,926 + Pf6N2E2_1619 0.17 +1.5 1,892,927 - Pf6N2E2_1619 0.17 +0.0 1,892,995 - Pf6N2E2_1619 0.25 +0.2 1,892,995 - Pf6N2E2_1619 0.25 -0.6 1,893,278 - Pf6N2E2_1619 0.59 +1.3 1,893,304 + Pf6N2E2_1619 0.62 +0.1 1,893,397 + Pf6N2E2_1619 0.73 +0.1 1,893,397 + Pf6N2E2_1619 0.73 +0.1 1,893,431 - Pf6N2E2_1619 0.77 -1.4 1,893,457 + Pf6N2E2_1619 0.80 -0.6 1,893,490 + Pf6N2E2_1619 0.84 -0.4 1,893,491 - Pf6N2E2_1619 0.84 -1.1 1,893,491 - Pf6N2E2_1619 0.84 -1.1 1,893,491 - Pf6N2E2_1619 0.84 +0.4 1,893,732 + +0.4 1,893,732 + -0.2 1,893,733 - -0.4 1,893,753 + -0.3 1,893,781 + +1.0 1,893,781 + -0.1 1,893,782 - +0.3 1,893,907 - Pf6N2E2_1620 0.18 -0.4 1,893,907 - Pf6N2E2_1620 0.18 -0.4 1,893,956 + Pf6N2E2_1620 0.22 +1.2 1,893,996 + Pf6N2E2_1620 0.25 +0.3 1,894,203 - Pf6N2E2_1620 0.40 +0.1 1,894,617 + Pf6N2E2_1620 0.69 +0.2 1,894,618 - Pf6N2E2_1620 0.69 +0.5
Or see this region's nucleotide sequence