Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1580 and Pf6N2E2_1581 are separated by 548 nucleotides Pf6N2E2_1581 and Pf6N2E2_1582 are separated by 2 nucleotides
Pf6N2E2_1580 - putative two-component system response regulator, at 1,848,837 to 1,849,454
_1580
Pf6N2E2_1581 - Type I secretion outer membrane protein, TolC precursor, at 1,850,003 to 1,851,376
_1581
Pf6N2E2_1582 - HlyD family secretion protein, at 1,851,379 to 1,852,695
_1582
Position (kb)
1850
1851
1852 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.013 kb on - strand, within Pf6N2E2_1580 at 1849.013 kb on - strand, within Pf6N2E2_1580 at 1849.282 kb on + strand, within Pf6N2E2_1580 at 1849.424 kb on + strand at 1849.426 kb on + strand at 1849.426 kb on + strand at 1849.426 kb on + strand at 1849.541 kb on + strand at 1849.541 kb on + strand at 1849.682 kb on - strand at 1849.691 kb on - strand at 1849.869 kb on + strand at 1850.057 kb on + strand at 1850.057 kb on + strand at 1850.058 kb on - strand at 1850.059 kb on + strand at 1850.377 kb on - strand, within Pf6N2E2_1581 at 1850.377 kb on - strand, within Pf6N2E2_1581 at 1850.415 kb on + strand, within Pf6N2E2_1581 at 1850.415 kb on + strand, within Pf6N2E2_1581 at 1850.435 kb on + strand, within Pf6N2E2_1581 at 1850.435 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.481 kb on + strand, within Pf6N2E2_1581 at 1850.835 kb on + strand, within Pf6N2E2_1581 at 1850.835 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.016 kb on - strand, within Pf6N2E2_1581 at 1851.046 kb on - strand, within Pf6N2E2_1581 at 1851.066 kb on + strand, within Pf6N2E2_1581 at 1851.067 kb on - strand, within Pf6N2E2_1581 at 1851.204 kb on + strand, within Pf6N2E2_1581 at 1851.205 kb on - strand, within Pf6N2E2_1581 at 1851.344 kb on + strand at 1851.344 kb on + strand at 1851.344 kb on + strand at 1851.345 kb on - strand at 1851.345 kb on - strand at 1851.345 kb on - strand at 1851.432 kb on - strand at 1851.548 kb on + strand, within Pf6N2E2_1582 at 1851.549 kb on - strand, within Pf6N2E2_1582 at 1851.835 kb on - strand, within Pf6N2E2_1582 at 1851.881 kb on + strand, within Pf6N2E2_1582 at 1851.881 kb on + strand, within Pf6N2E2_1582 at 1852.118 kb on + strand, within Pf6N2E2_1582
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,849,012 + Pf6N2E2_1580 0.28 +0.5 1,849,012 + Pf6N2E2_1580 0.28 +0.1 1,849,012 + Pf6N2E2_1580 0.28 +1.1 1,849,013 - Pf6N2E2_1580 0.28 -0.2 1,849,013 - Pf6N2E2_1580 0.28 +0.6 1,849,282 + Pf6N2E2_1580 0.72 +0.3 1,849,424 + +0.6 1,849,426 + -0.2 1,849,426 + -0.1 1,849,426 + +0.3 1,849,541 + +0.2 1,849,541 + +1.1 1,849,682 - -0.7 1,849,691 - -1.2 1,849,869 + +0.5 1,850,057 + -0.0 1,850,057 + +2.0 1,850,058 - +0.2 1,850,059 + +0.2 1,850,377 - Pf6N2E2_1581 0.27 +0.6 1,850,377 - Pf6N2E2_1581 0.27 -1.3 1,850,415 + Pf6N2E2_1581 0.30 +0.4 1,850,415 + Pf6N2E2_1581 0.30 -0.0 1,850,435 + Pf6N2E2_1581 0.31 +2.2 1,850,435 + Pf6N2E2_1581 0.31 +0.4 1,850,448 + Pf6N2E2_1581 0.32 -1.9 1,850,448 + Pf6N2E2_1581 0.32 +0.9 1,850,448 + Pf6N2E2_1581 0.32 +0.6 1,850,448 + Pf6N2E2_1581 0.32 -0.9 1,850,448 + Pf6N2E2_1581 0.32 -1.6 1,850,448 + Pf6N2E2_1581 0.32 -0.2 1,850,449 - Pf6N2E2_1581 0.32 +0.5 1,850,449 - Pf6N2E2_1581 0.32 +0.5 1,850,449 - Pf6N2E2_1581 0.32 +0.7 1,850,481 + Pf6N2E2_1581 0.35 -0.1 1,850,835 + Pf6N2E2_1581 0.61 +1.3 1,850,835 + Pf6N2E2_1581 0.61 +0.7 1,850,928 + Pf6N2E2_1581 0.67 -0.3 1,850,928 + Pf6N2E2_1581 0.67 +0.4 1,850,928 + Pf6N2E2_1581 0.67 -0.2 1,851,015 + Pf6N2E2_1581 0.74 -0.3 1,851,015 + Pf6N2E2_1581 0.74 +0.7 1,851,015 + Pf6N2E2_1581 0.74 +0.1 1,851,016 - Pf6N2E2_1581 0.74 -1.2 1,851,046 - Pf6N2E2_1581 0.76 -0.0 1,851,066 + Pf6N2E2_1581 0.77 -2.1 1,851,067 - Pf6N2E2_1581 0.77 +0.6 1,851,204 + Pf6N2E2_1581 0.87 +0.1 1,851,205 - Pf6N2E2_1581 0.87 -0.1 1,851,344 + +0.4 1,851,344 + -0.3 1,851,344 + +0.5 1,851,345 - +0.1 1,851,345 - -0.6 1,851,345 - +0.6 1,851,432 - -0.0 1,851,548 + Pf6N2E2_1582 0.13 +0.3 1,851,549 - Pf6N2E2_1582 0.13 -0.9 1,851,835 - Pf6N2E2_1582 0.35 +1.2 1,851,881 + Pf6N2E2_1582 0.38 -1.2 1,851,881 + Pf6N2E2_1582 0.38 +0.2 1,852,118 + Pf6N2E2_1582 0.56 +0.8
Or see this region's nucleotide sequence