Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1439 and Pf6N2E2_1440 are separated by 469 nucleotides Pf6N2E2_1440 and Pf6N2E2_1441 are separated by 15 nucleotides
Pf6N2E2_1439 - hypothetical protein, at 1,689,370 to 1,689,948
_1439
Pf6N2E2_1440 - Putative tRNA-m1A22 methylase, at 1,690,418 to 1,691,113
_1440
Pf6N2E2_1441 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 1,691,129 to 1,692,055
_1441
Position (kb)
1690
1691
1692 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1689.445 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.706 kb on + strand, within Pf6N2E2_1439 at 1689.707 kb on - strand, within Pf6N2E2_1439 at 1689.781 kb on + strand, within Pf6N2E2_1439 at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.227 kb on + strand at 1690.228 kb on - strand at 1690.277 kb on - strand at 1690.277 kb on - strand at 1690.330 kb on - strand at 1690.330 kb on - strand at 1690.340 kb on + strand at 1690.351 kb on - strand at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.614 kb on + strand, within Pf6N2E2_1440 at 1690.615 kb on - strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1691.020 kb on - strand, within Pf6N2E2_1440 at 1691.065 kb on + strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.137 kb on - strand at 1691.251 kb on - strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.355 kb on - strand, within Pf6N2E2_1441 at 1692.079 kb on - strand at 1692.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,689,445 + Pf6N2E2_1439 0.13 +0.8 1,689,481 + Pf6N2E2_1439 0.19 -1.1 1,689,481 + Pf6N2E2_1439 0.19 -0.1 1,689,481 + Pf6N2E2_1439 0.19 +1.1 1,689,481 + Pf6N2E2_1439 0.19 +0.8 1,689,482 - Pf6N2E2_1439 0.19 +0.3 1,689,482 - Pf6N2E2_1439 0.19 +0.3 1,689,482 - Pf6N2E2_1439 0.19 +1.7 1,689,672 - Pf6N2E2_1439 0.52 +0.2 1,689,672 - Pf6N2E2_1439 0.52 +0.9 1,689,685 + Pf6N2E2_1439 0.54 -0.1 1,689,685 + Pf6N2E2_1439 0.54 -0.2 1,689,685 + Pf6N2E2_1439 0.54 -0.3 1,689,685 + Pf6N2E2_1439 0.54 +0.6 1,689,685 + Pf6N2E2_1439 0.54 +0.4 1,689,686 - Pf6N2E2_1439 0.55 +0.7 1,689,686 - Pf6N2E2_1439 0.55 +0.5 1,689,686 - Pf6N2E2_1439 0.55 +0.4 1,689,686 - Pf6N2E2_1439 0.55 +1.0 1,689,706 + Pf6N2E2_1439 0.58 -0.1 1,689,707 - Pf6N2E2_1439 0.58 +0.4 1,689,781 + Pf6N2E2_1439 0.71 +0.5 1,690,094 - -0.2 1,690,094 - +0.2 1,690,094 - -0.7 1,690,094 - -0.2 1,690,094 - +0.3 1,690,094 - +0.8 1,690,098 - +0.2 1,690,098 - +0.6 1,690,098 - +0.9 1,690,227 + -0.0 1,690,228 - -0.8 1,690,277 - +0.4 1,690,277 - +0.2 1,690,330 - +0.3 1,690,330 - -0.1 1,690,340 + -0.6 1,690,351 - -0.3 1,690,596 + Pf6N2E2_1440 0.26 -1.3 1,690,596 + Pf6N2E2_1440 0.26 -3.0 1,690,614 + Pf6N2E2_1440 0.28 +0.2 1,690,615 - Pf6N2E2_1440 0.28 -1.1 1,690,751 + Pf6N2E2_1440 0.48 +0.7 1,690,751 + Pf6N2E2_1440 0.48 -1.5 1,690,752 - Pf6N2E2_1440 0.48 +0.7 1,690,752 - Pf6N2E2_1440 0.48 -1.3 1,691,020 - Pf6N2E2_1440 0.86 -0.7 1,691,065 + +0.1 1,691,066 - +0.7 1,691,066 - +0.2 1,691,066 - +0.9 1,691,137 - -0.7 1,691,251 - Pf6N2E2_1441 0.13 +0.5 1,691,354 + Pf6N2E2_1441 0.24 +0.4 1,691,354 + Pf6N2E2_1441 0.24 -0.2 1,691,355 - Pf6N2E2_1441 0.24 +0.1 1,692,079 - +0.3 1,692,101 - +0.5
Or see this region's nucleotide sequence