Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1416

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1415 and Pf6N2E2_1416 are separated by 300 nucleotidesPf6N2E2_1416 and Pf6N2E2_1417 are separated by 90 nucleotidesPf6N2E2_1417 and Pf6N2E2_1418 are separated by 174 nucleotidesPf6N2E2_1418 and Pf6N2E2_1419 are separated by 174 nucleotides Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496 _1415 Pf6N2E2_1416 - Type I secretion outer membrane protein, TolC precursor, at 1,664,797 to 1,665,003 _1416 Pf6N2E2_1417 - Type I secretion outer membrane protein, TolC precursor, at 1,665,094 to 1,665,249 _1417 Pf6N2E2_1418 - Type I secretion outer membrane protein, TolC precursor, at 1,665,424 to 1,665,579 _1418 Pf6N2E2_1419 - Type I secretion outer membrane protein, TolC precursor, at 1,665,754 to 1,667,103 _1419 Position (kb) 1664 1665 1666Strain fitness (log2 ratio) -2 -1 0 1at 1663.822 kb on + strand, within Pf6N2E2_1415at 1663.823 kb on - strand, within Pf6N2E2_1415at 1663.872 kb on + strand, within Pf6N2E2_1415at 1663.873 kb on - strand, within Pf6N2E2_1415at 1663.873 kb on - strand, within Pf6N2E2_1415at 1664.197 kb on + strandat 1664.197 kb on + strandat 1664.197 kb on + strandat 1664.198 kb on - strandat 1664.471 kb on + strandat 1664.471 kb on + strandat 1664.472 kb on - strandat 1664.523 kb on + strandat 1664.523 kb on + strandat 1664.524 kb on - strandat 1664.527 kb on + strandat 1664.528 kb on - strandat 1664.999 kb on - strandat 1665.990 kb on + strand, within Pf6N2E2_1419at 1665.991 kb on - strand, within Pf6N2E2_1419at 1665.991 kb on - strand, within Pf6N2E2_1419at 1665.991 kb on - strand, within Pf6N2E2_1419

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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1,663,822 + Pf6N2E2_1415 0.88 -0.6
1,663,823 - Pf6N2E2_1415 0.88 +0.4
1,663,872 + Pf6N2E2_1415 0.89 +0.4
1,663,873 - Pf6N2E2_1415 0.89 -0.3
1,663,873 - Pf6N2E2_1415 0.89 +0.4
1,664,197 + -0.5
1,664,197 + -0.9
1,664,197 + +0.5
1,664,198 - -0.8
1,664,471 + +0.3
1,664,471 + +0.5
1,664,472 - -2.5
1,664,523 + +0.3
1,664,523 + -0.1
1,664,524 - +0.4
1,664,527 + -0.0
1,664,528 - -0.4
1,664,999 - +0.7
1,665,990 + Pf6N2E2_1419 0.17 -0.1
1,665,991 - Pf6N2E2_1419 0.18 -0.2
1,665,991 - Pf6N2E2_1419 0.18 -0.1
1,665,991 - Pf6N2E2_1419 0.18 -0.7

Or see this region's nucleotide sequence