Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1415 and Pf6N2E2_1416 are separated by 300 nucleotides Pf6N2E2_1416 and Pf6N2E2_1417 are separated by 90 nucleotides Pf6N2E2_1417 and Pf6N2E2_1418 are separated by 174 nucleotides Pf6N2E2_1418 and Pf6N2E2_1419 are separated by 174 nucleotides
Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496
_1415
Pf6N2E2_1416 - Type I secretion outer membrane protein, TolC precursor, at 1,664,797 to 1,665,003
_1416
Pf6N2E2_1417 - Type I secretion outer membrane protein, TolC precursor, at 1,665,094 to 1,665,249
_1417
Pf6N2E2_1418 - Type I secretion outer membrane protein, TolC precursor, at 1,665,424 to 1,665,579
_1418
Pf6N2E2_1419 - Type I secretion outer membrane protein, TolC precursor, at 1,665,754 to 1,667,103
_1419
Position (kb)
1664
1665
1666 Strain fitness (log2 ratio)
-2
-1
0
1 at 1663.822 kb on + strand, within Pf6N2E2_1415 at 1663.823 kb on - strand, within Pf6N2E2_1415 at 1663.872 kb on + strand, within Pf6N2E2_1415 at 1663.873 kb on - strand, within Pf6N2E2_1415 at 1663.873 kb on - strand, within Pf6N2E2_1415 at 1664.197 kb on + strand at 1664.197 kb on + strand at 1664.197 kb on + strand at 1664.198 kb on - strand at 1664.471 kb on + strand at 1664.471 kb on + strand at 1664.472 kb on - strand at 1664.523 kb on + strand at 1664.523 kb on + strand at 1664.524 kb on - strand at 1664.527 kb on + strand at 1664.528 kb on - strand at 1664.999 kb on - strand at 1665.990 kb on + strand, within Pf6N2E2_1419 at 1665.991 kb on - strand, within Pf6N2E2_1419 at 1665.991 kb on - strand, within Pf6N2E2_1419 at 1665.991 kb on - strand, within Pf6N2E2_1419
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,663,822 + Pf6N2E2_1415 0.88 -0.6 1,663,823 - Pf6N2E2_1415 0.88 +0.4 1,663,872 + Pf6N2E2_1415 0.89 +0.4 1,663,873 - Pf6N2E2_1415 0.89 -0.3 1,663,873 - Pf6N2E2_1415 0.89 +0.4 1,664,197 + -0.5 1,664,197 + -0.9 1,664,197 + +0.5 1,664,198 - -0.8 1,664,471 + +0.3 1,664,471 + +0.5 1,664,472 - -2.5 1,664,523 + +0.3 1,664,523 + -0.1 1,664,524 - +0.4 1,664,527 + -0.0 1,664,528 - -0.4 1,664,999 - +0.7 1,665,990 + Pf6N2E2_1419 0.17 -0.1 1,665,991 - Pf6N2E2_1419 0.18 -0.2 1,665,991 - Pf6N2E2_1419 0.18 -0.1 1,665,991 - Pf6N2E2_1419 0.18 -0.7
Or see this region's nucleotide sequence