Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3077 and mazG are separated by 115 nucleotides mazG and DvMF_3079 overlap by 1 nucleotides DvMF_3079 and DvMF_3080 are separated by 67 nucleotides DvMF_3080 and DvMF_3081 are separated by 214 nucleotides DvMF_3081 and DvMF_3082 are separated by 228 nucleotides
DvMF_3077: DvMF_3077 - Colicin V production protein (RefSeq), at 3,886,409 to 3,886,885
_3077
DvMF_3078: mazG - nucleoside triphosphate pyrophosphohydrolase (RefSeq), at 3,887,001 to 3,887,864
mazG
DvMF_3079: DvMF_3079 - hypothetical protein (RefSeq), at 3,887,864 to 3,889,366
_3079
DvMF_3080: DvMF_3080 - hypothetical protein (RefSeq), at 3,889,434 to 3,889,640
_3080
DvMF_3081: DvMF_3081 - hypothetical protein (RefSeq), at 3,889,855 to 3,890,037
_3081
DvMF_3082: DvMF_3082 - protein of unknown function UPF0118 (RefSeq), at 3,890,266 to 3,891,459
_3082
Position (kb)
3887
3888
3889
3890 Strain fitness (log2 ratio)
-1
0
1 at 3888.158 kb on - strand, within DvMF_3079 at 3888.287 kb on - strand, within DvMF_3079 at 3888.958 kb on - strand, within DvMF_3079 at 3889.022 kb on - strand, within DvMF_3079 at 3889.173 kb on + strand, within DvMF_3079 at 3889.408 kb on + strand at 3889.457 kb on + strand, within DvMF_3080 at 3889.457 kb on + strand, within DvMF_3080 at 3889.465 kb on - strand, within DvMF_3080 at 3889.489 kb on + strand, within DvMF_3080 at 3889.562 kb on - strand, within DvMF_3080 at 3889.741 kb on - strand at 3889.810 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 3,888,158 - DvMF_3079 0.20 -0.7 3,888,287 - DvMF_3079 0.28 -0.8 3,888,958 - DvMF_3079 0.73 -1.1 3,889,022 - DvMF_3079 0.77 -0.7 3,889,173 + DvMF_3079 0.87 -0.9 3,889,408 + -0.7 3,889,457 + DvMF_3080 0.11 +1.1 3,889,457 + DvMF_3080 0.11 -0.2 3,889,465 - DvMF_3080 0.15 -0.8 3,889,489 + DvMF_3080 0.27 -0.4 3,889,562 - DvMF_3080 0.62 -0.2 3,889,741 - -0.5 3,889,810 + -1.0
Or see this region's nucleotide sequence