Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE and DvMF_3008 are separated by 227 nucleotides DvMF_3008 and DvMF_3009 are separated by 99 nucleotides DvMF_3009 and DvMF_3010 are separated by 368 nucleotides
DvMF_3007: thiE - thiamine-phosphate pyrophosphorylase (RefSeq), at 3,794,425 to 3,795,066
thiE
DvMF_3008: DvMF_3008 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,795,294 to 3,795,629
_3008
DvMF_3009: DvMF_3009 - oxidoreductase FAD/NAD(P)-binding domain protein (RefSeq), at 3,795,729 to 3,796,493
_3009
DvMF_3010: DvMF_3010 - sigma54 specific transcriptional regulator, Fis family (RefSeq), at 3,796,862 to 3,798,667
_3010
Position (kb)
3795
3796
3797 Strain fitness (log2 ratio)
-1
0
1 at 3794.942 kb on - strand, within thiE at 3794.985 kb on - strand, within thiE at 3795.080 kb on - strand at 3795.129 kb on - strand at 3795.200 kb on + strand at 3795.221 kb on + strand at 3795.221 kb on + strand at 3795.701 kb on + strand at 3795.712 kb on + strand at 3795.720 kb on - strand at 3795.834 kb on + strand, within DvMF_3009 at 3795.925 kb on + strand, within DvMF_3009 at 3796.560 kb on - strand at 3796.630 kb on + strand at 3797.297 kb on - strand, within DvMF_3010
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 3,794,942 - thiE DvMF_3007 0.81 -0.8 3,794,985 - thiE DvMF_3007 0.87 -1.1 3,795,080 - +0.3 3,795,129 - -1.1 3,795,200 + -1.0 3,795,221 + -0.5 3,795,221 + -0.4 3,795,701 + +0.2 3,795,712 + -1.0 3,795,720 - -0.8 3,795,834 + DvMF_3009 0.14 -0.2 3,795,925 + DvMF_3009 0.26 -0.7 3,796,560 - -0.6 3,796,630 + +0.1 3,797,297 - DvMF_3010 0.24 +0.3
Or see this region's nucleotide sequence