Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2867 and DvMF_2868 are separated by 209 nucleotides DvMF_2868 and DvMF_2869 are separated by 100 nucleotides DvMF_2869 and DvMF_2870 are separated by 325 nucleotides
DvMF_2867: DvMF_2867 - protein of unknown function DUF847 (RefSeq), at 3,620,910 to 3,621,488
_2867
DvMF_2868: DvMF_2868 - Transglycosylase-associated protein (RefSeq), at 3,621,698 to 3,621,940
_2868
DvMF_2869: DvMF_2869 - hypothetical protein (RefSeq), at 3,622,041 to 3,622,559
_2869
DvMF_2870: DvMF_2870 - OmpA/MotB domain protein (RefSeq), at 3,622,885 to 3,623,631
_2870
Position (kb)
3621
3622 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3620.796 kb on - strand at 3620.873 kb on - strand at 3620.889 kb on - strand at 3620.963 kb on - strand at 3621.131 kb on - strand, within DvMF_2867 at 3621.213 kb on - strand, within DvMF_2867 at 3621.239 kb on + strand, within DvMF_2867 at 3621.254 kb on - strand, within DvMF_2867 at 3621.293 kb on + strand, within DvMF_2867 at 3621.343 kb on - strand, within DvMF_2867 at 3621.771 kb on + strand, within DvMF_2868 at 3622.155 kb on + strand, within DvMF_2869 at 3622.403 kb on + strand, within DvMF_2869 at 3622.516 kb on - strand at 3622.622 kb on + strand at 3622.669 kb on + strand at 3622.700 kb on - strand at 3622.876 kb on + strand at 3622.876 kb on + strand at 3622.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 3,620,796 - -0.1 3,620,873 - -1.4 3,620,889 - -0.0 3,620,963 - +1.6 3,621,131 - DvMF_2867 0.38 -0.3 3,621,213 - DvMF_2867 0.52 -0.7 3,621,239 + DvMF_2867 0.57 -1.0 3,621,254 - DvMF_2867 0.59 -0.3 3,621,293 + DvMF_2867 0.66 -0.4 3,621,343 - DvMF_2867 0.75 -3.2 3,621,771 + DvMF_2868 0.30 -1.6 3,622,155 + DvMF_2869 0.22 -1.8 3,622,403 + DvMF_2869 0.70 -0.3 3,622,516 - -0.1 3,622,622 + +2.5 3,622,669 + -0.6 3,622,700 - -0.5 3,622,876 + -0.7 3,622,876 + -0.7 3,622,886 - +1.8
Or see this region's nucleotide sequence