Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1908 and DvMF_1909 are separated by 263 nucleotides DvMF_1909 and DvMF_1910 are separated by 25 nucleotides DvMF_1910 and DvMF_1911 are separated by 174 nucleotides
DvMF_1908: DvMF_1908 - glucose-1-phosphate cytidylyltransferase (RefSeq), at 2,352,430 to 2,353,212
_1908
DvMF_1909: DvMF_1909 - CDP-glucose 4,6-dehydratase (RefSeq), at 2,353,476 to 2,354,585
_1909
DvMF_1910: DvMF_1910 - polysaccharide biosynthesis domain-containing protein (RefSeq), at 2,354,611 to 2,355,060
_1910
DvMF_1911: DvMF_1911 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,355,235 to 2,356,368
_1911
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 2352.663 kb on + strand, within DvMF_1908 at 2352.742 kb on + strand, within DvMF_1908 at 2352.767 kb on - strand, within DvMF_1908 at 2353.362 kb on - strand at 2353.418 kb on + strand at 2353.658 kb on - strand, within DvMF_1909 at 2353.688 kb on + strand, within DvMF_1909 at 2353.688 kb on + strand, within DvMF_1909 at 2353.943 kb on - strand, within DvMF_1909 at 2353.943 kb on - strand, within DvMF_1909 at 2354.180 kb on + strand, within DvMF_1909 at 2354.299 kb on + strand, within DvMF_1909 at 2354.616 kb on - strand at 2355.096 kb on + strand at 2355.374 kb on + strand, within DvMF_1911
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 2,352,663 + DvMF_1908 0.30 +8.8 2,352,742 + DvMF_1908 0.40 +10.0 2,352,767 - DvMF_1908 0.43 +9.9 2,353,362 - -1.2 2,353,418 + -0.2 2,353,658 - DvMF_1909 0.16 +10.1 2,353,688 + DvMF_1909 0.19 +10.9 2,353,688 + DvMF_1909 0.19 +10.4 2,353,943 - DvMF_1909 0.42 +11.3 2,353,943 - DvMF_1909 0.42 +10.5 2,354,180 + DvMF_1909 0.63 +11.1 2,354,299 + DvMF_1909 0.74 +8.5 2,354,616 - +1.6 2,355,096 + -1.9 2,355,374 + DvMF_1911 0.12 +9.4
Or see this region's nucleotide sequence