Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1114 and DvMF_1115 are separated by 475 nucleotides DvMF_1115 and DvMF_1116 are separated by 168 nucleotides DvMF_1116 and DvMF_1117 are separated by 370 nucleotides
DvMF_1114: DvMF_1114 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 1,363,419 to 1,365,521
_1114
DvMF_1115: DvMF_1115 - thioesterase superfamily protein (RefSeq), at 1,365,997 to 1,366,413
_1115
DvMF_1116: DvMF_1116 - Threonyl/alanyl tRNA synthetase SAD (RefSeq), at 1,366,582 to 1,367,028
_1116
DvMF_1117: DvMF_1117 - heat shock protein 90 (RefSeq), at 1,367,399 to 1,369,405
_1117
Position (kb)
1365
1366
1367 Strain fitness (log2 ratio)
-1
0
1 at 1365.052 kb on - strand, within DvMF_1114 at 1365.149 kb on - strand, within DvMF_1114 at 1365.192 kb on - strand, within DvMF_1114 at 1365.360 kb on + strand at 1365.587 kb on + strand at 1365.630 kb on - strand at 1365.676 kb on + strand at 1365.758 kb on - strand at 1366.145 kb on + strand, within DvMF_1115 at 1366.167 kb on + strand, within DvMF_1115 at 1366.212 kb on + strand, within DvMF_1115 at 1366.317 kb on - strand, within DvMF_1115 at 1366.322 kb on - strand, within DvMF_1115 at 1366.352 kb on - strand, within DvMF_1115 at 1366.812 kb on - strand, within DvMF_1116 at 1366.963 kb on + strand, within DvMF_1116 at 1367.056 kb on - strand at 1367.203 kb on + strand at 1367.358 kb on - strand at 1367.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 1,365,052 - DvMF_1114 0.78 -0.7 1,365,149 - DvMF_1114 0.82 -0.2 1,365,192 - DvMF_1114 0.84 -0.6 1,365,360 + -0.4 1,365,587 + -1.0 1,365,630 - -0.2 1,365,676 + -1.4 1,365,758 - -1.0 1,366,145 + DvMF_1115 0.35 -1.4 1,366,167 + DvMF_1115 0.41 -1.2 1,366,212 + DvMF_1115 0.52 -1.1 1,366,317 - DvMF_1115 0.77 -1.5 1,366,322 - DvMF_1115 0.78 -0.2 1,366,352 - DvMF_1115 0.85 -0.3 1,366,812 - DvMF_1116 0.51 +0.3 1,366,963 + DvMF_1116 0.85 -1.4 1,367,056 - -0.1 1,367,203 + -0.7 1,367,358 - -0.3 1,367,359 - -1.1
Or see this region's nucleotide sequence