Experiment: MoYLS4 with Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0851 and DvMF_0852 are separated by 160 nucleotides DvMF_0852 and DvMF_0853 are separated by 12 nucleotides DvMF_0853 and DvMF_0854 are separated by 108 nucleotides
DvMF_0851: DvMF_0851 - Sporulation domain protein (RefSeq), at 1,029,357 to 1,030,292
_0851
DvMF_0852: DvMF_0852 - protein of unknown function DUF140 (RefSeq), at 1,030,453 to 1,031,256
_0852
DvMF_0853: DvMF_0853 - ABC transporter related (RefSeq), at 1,031,269 to 1,032,090
_0853
DvMF_0854: DvMF_0854 - Mammalian cell entry related domain protein (RefSeq), at 1,032,199 to 1,032,645
_0854
Position (kb)
1030
1031
1032 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1029.724 kb on - strand, within DvMF_0851 at 1029.814 kb on - strand, within DvMF_0851 at 1030.190 kb on + strand, within DvMF_0851 at 1030.372 kb on - strand at 1030.476 kb on - strand at 1030.680 kb on - strand, within DvMF_0852 at 1030.988 kb on + strand, within DvMF_0852 at 1030.996 kb on - strand, within DvMF_0852 at 1031.040 kb on - strand, within DvMF_0852 at 1031.557 kb on - strand, within DvMF_0853 at 1031.562 kb on - strand, within DvMF_0853
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 1 mg/ml remove 1,029,724 - DvMF_0851 0.39 +0.3 1,029,814 - DvMF_0851 0.49 +1.3 1,030,190 + DvMF_0851 0.89 +0.0 1,030,372 - +0.8 1,030,476 - +2.3 1,030,680 - DvMF_0852 0.28 +2.9 1,030,988 + DvMF_0852 0.67 +2.7 1,030,996 - DvMF_0852 0.68 +5.4 1,031,040 - DvMF_0852 0.73 +3.3 1,031,557 - DvMF_0853 0.35 +1.1 1,031,562 - DvMF_0853 0.36 +2.0
Or see this region's nucleotide sequence