Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides MMP_RS00745 and MMP_RS00750 are separated by 15 nucleotides MMP_RS00750 and MMP_RS00755 are separated by 144 nucleotides
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
MMP_RS00750: MMP_RS00750 - fumarate hydratase, at 136,315 to 137,160
_RS00750
MMP_RS00755: MMP_RS00755 - tyrosine decarboxylase MfnA, at 137,305 to 138,459
_RS00755
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand at 135.800 kb on - strand at 136.395 kb on + strand at 136.454 kb on - strand, within MMP_RS00750 at 136.665 kb on - strand, within MMP_RS00750 at 136.688 kb on - strand, within MMP_RS00750 at 136.868 kb on - strand, within MMP_RS00750 at 137.061 kb on + strand, within MMP_RS00750 at 137.179 kb on + strand at 137.207 kb on - strand at 137.522 kb on - strand at 137.551 kb on + strand at 137.829 kb on - strand at 137.933 kb on - strand at 137.934 kb on + strand at 137.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 135,338 + MMP_RS00740 0.60 -0.2 135,421 - MMP_RS00740 0.75 -0.1 135,422 + MMP_RS00740 0.75 +0.2 135,565 - +0.6 135,600 + -1.6 135,606 + -0.0 135,613 - -2.8 135,613 - +0.1 135,619 + -1.1 135,675 - -1.3 135,800 - -1.8 136,395 + -0.8 136,454 - MMP_RS00750 0.16 -1.6 136,665 - MMP_RS00750 0.41 -0.7 136,688 - MMP_RS00750 0.44 -1.2 136,868 - MMP_RS00750 0.65 -0.8 137,061 + MMP_RS00750 0.88 -1.1 137,179 + +0.1 137,207 - -0.2 137,522 - -0.0 137,551 + -1.2 137,829 - -1.3 137,933 - -0.6 137,934 + -1.2 137,943 - -0.7
Or see this region's nucleotide sequence