Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00115 and MMP_RS00120 are separated by 72 nucleotides MMP_RS00120 and MMP_RS00125 are separated by 29 nucleotides MMP_RS00125 and MMP_RS00130 are separated by 91 nucleotides MMP_RS00130 and MMP_RS00135 are separated by 116 nucleotides
MMP_RS00115: MMP_RS00115 - radical SAM protein, at 26,673 to 27,725
_RS00115
MMP_RS00120: MMP_RS00120 - LysR family transcriptional regulator, at 27,798 to 28,640
_RS00120
MMP_RS00125: MMP_RS00125 - DUF2798 domain-containing protein, at 28,670 to 28,897
_RS00125
MMP_RS00130: MMP_RS00130 - nickel-responsive transcriptional regulator NikR, at 28,989 to 29,414
_RS00130
MMP_RS00135: MMP_RS00135 - DUF2121 domain-containing protein, at 29,531 to 30,406
_RS00135
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 26.829 kb on - strand, within MMP_RS00115 at 26.852 kb on - strand, within MMP_RS00115 at 26.855 kb on + strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.915 kb on + strand, within MMP_RS00115 at 26.939 kb on - strand, within MMP_RS00115 at 26.940 kb on + strand, within MMP_RS00115 at 26.944 kb on + strand, within MMP_RS00115 at 26.981 kb on - strand, within MMP_RS00115 at 26.987 kb on - strand, within MMP_RS00115 at 27.057 kb on + strand, within MMP_RS00115 at 27.209 kb on - strand, within MMP_RS00115 at 27.387 kb on - strand, within MMP_RS00115 at 27.413 kb on + strand, within MMP_RS00115 at 27.473 kb on - strand, within MMP_RS00115 at 27.492 kb on - strand, within MMP_RS00115 at 27.493 kb on + strand, within MMP_RS00115 at 27.596 kb on - strand, within MMP_RS00115 at 27.614 kb on - strand, within MMP_RS00115 at 27.650 kb on - strand at 27.725 kb on - strand at 27.728 kb on - strand at 27.739 kb on + strand at 27.764 kb on - strand at 27.765 kb on + strand at 27.823 kb on - strand at 27.824 kb on + strand at 27.840 kb on + strand at 27.842 kb on + strand at 27.846 kb on - strand at 27.846 kb on - strand at 27.847 kb on + strand at 27.847 kb on + strand at 27.848 kb on - strand at 28.059 kb on - strand, within MMP_RS00120 at 28.060 kb on + strand, within MMP_RS00120 at 28.071 kb on - strand, within MMP_RS00120 at 28.115 kb on - strand, within MMP_RS00120 at 28.118 kb on + strand, within MMP_RS00120 at 28.332 kb on - strand, within MMP_RS00120 at 28.609 kb on - strand at 28.657 kb on + strand at 28.659 kb on - strand at 28.660 kb on + strand at 28.683 kb on + strand at 28.829 kb on - strand, within MMP_RS00125 at 28.900 kb on + strand at 28.900 kb on + strand at 28.924 kb on - strand at 29.300 kb on - strand, within MMP_RS00130 at 29.390 kb on - strand at 29.390 kb on - strand at 29.390 kb on - strand at 29.417 kb on - strand at 29.417 kb on - strand at 29.418 kb on + strand at 29.453 kb on + strand at 29.459 kb on - strand at 29.516 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 26,829 - MMP_RS00115 0.15 -1.4 26,852 - MMP_RS00115 0.17 -1.7 26,855 + MMP_RS00115 0.17 -1.4 26,856 - MMP_RS00115 0.17 -1.3 26,856 - MMP_RS00115 0.17 -1.1 26,856 - MMP_RS00115 0.17 -1.6 26,856 - MMP_RS00115 0.17 -1.8 26,856 - MMP_RS00115 0.17 -2.8 26,857 + MMP_RS00115 0.17 -1.3 26,857 + MMP_RS00115 0.17 -2.1 26,857 + MMP_RS00115 0.17 -1.6 26,857 + MMP_RS00115 0.17 -0.7 26,915 + MMP_RS00115 0.23 -1.6 26,939 - MMP_RS00115 0.25 -1.7 26,940 + MMP_RS00115 0.25 -2.0 26,944 + MMP_RS00115 0.26 -1.2 26,981 - MMP_RS00115 0.29 -1.9 26,987 - MMP_RS00115 0.30 -1.3 27,057 + MMP_RS00115 0.36 -1.6 27,209 - MMP_RS00115 0.51 -1.7 27,387 - MMP_RS00115 0.68 -1.6 27,413 + MMP_RS00115 0.70 -1.2 27,473 - MMP_RS00115 0.76 -1.5 27,492 - MMP_RS00115 0.78 -1.7 27,493 + MMP_RS00115 0.78 -1.5 27,596 - MMP_RS00115 0.88 -0.2 27,614 - MMP_RS00115 0.89 -1.8 27,650 - -0.3 27,725 - -1.3 27,728 - -0.9 27,739 + -1.6 27,764 - -0.0 27,765 + -1.3 27,823 - -1.6 27,824 + -3.5 27,840 + -1.8 27,842 + -1.4 27,846 - -0.5 27,846 - -1.4 27,847 + -1.0 27,847 + -0.9 27,848 - -1.5 28,059 - MMP_RS00120 0.31 -0.7 28,060 + MMP_RS00120 0.31 -1.7 28,071 - MMP_RS00120 0.32 -0.9 28,115 - MMP_RS00120 0.38 -0.6 28,118 + MMP_RS00120 0.38 -0.5 28,332 - MMP_RS00120 0.63 -0.3 28,609 - -1.8 28,657 + -0.0 28,659 - -0.2 28,660 + +0.1 28,683 + -0.1 28,829 - MMP_RS00125 0.70 +0.1 28,900 + +0.0 28,900 + -0.1 28,924 - +0.0 29,300 - MMP_RS00130 0.73 -0.5 29,390 - -0.1 29,390 - -0.3 29,390 - -0.2 29,417 - +0.2 29,417 - +0.2 29,418 + -0.4 29,453 + -0.5 29,459 - -0.1 29,516 + -0.2
Or see this region's nucleotide sequence