Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05358 and H281DRAFT_05359 are separated by 75 nucleotides H281DRAFT_05359 and H281DRAFT_05360 overlap by 4 nucleotides H281DRAFT_05360 and H281DRAFT_05361 are separated by 121 nucleotides
H281DRAFT_05358: H281DRAFT_05358 - glucose-6-phosphate 1-dehydrogenase, at 110,843 to 112,324
_05358
H281DRAFT_05359: H281DRAFT_05359 - type VI secretion system protein ImpK, at 112,400 to 113,179
_05359
H281DRAFT_05360: H281DRAFT_05360 - type VI secretion system protein ImpJ, at 113,176 to 114,522
_05360
H281DRAFT_05361: H281DRAFT_05361 - type VI secretion system protein VasD, at 114,644 to 115,309
_05361
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 112.241 kb on + strand at 112.242 kb on - strand at 112.242 kb on - strand at 112.323 kb on - strand at 112.323 kb on - strand at 112.381 kb on + strand at 112.381 kb on + strand at 112.494 kb on + strand, within H281DRAFT_05359 at 112.495 kb on - strand, within H281DRAFT_05359 at 112.495 kb on - strand, within H281DRAFT_05359 at 112.495 kb on - strand, within H281DRAFT_05359 at 112.674 kb on + strand, within H281DRAFT_05359 at 112.674 kb on + strand, within H281DRAFT_05359 at 112.704 kb on + strand, within H281DRAFT_05359 at 112.704 kb on + strand, within H281DRAFT_05359 at 112.704 kb on + strand, within H281DRAFT_05359 at 112.704 kb on + strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.705 kb on - strand, within H281DRAFT_05359 at 112.795 kb on - strand, within H281DRAFT_05359 at 112.935 kb on + strand, within H281DRAFT_05359 at 112.936 kb on - strand, within H281DRAFT_05359 at 112.936 kb on - strand, within H281DRAFT_05359 at 113.077 kb on - strand, within H281DRAFT_05359 at 113.077 kb on - strand, within H281DRAFT_05359 at 113.106 kb on + strand at 113.107 kb on - strand at 113.172 kb on + strand at 113.231 kb on + strand at 113.231 kb on + strand at 113.231 kb on + strand at 113.231 kb on + strand at 113.231 kb on + strand at 113.232 kb on - strand at 113.232 kb on - strand at 113.232 kb on - strand at 113.232 kb on - strand at 113.267 kb on + strand at 113.268 kb on - strand at 113.297 kb on + strand at 113.298 kb on - strand at 113.345 kb on + strand, within H281DRAFT_05360 at 113.472 kb on + strand, within H281DRAFT_05360 at 113.473 kb on - strand, within H281DRAFT_05360 at 113.474 kb on + strand, within H281DRAFT_05360 at 113.474 kb on + strand, within H281DRAFT_05360 at 113.475 kb on - strand, within H281DRAFT_05360 at 113.475 kb on - strand, within H281DRAFT_05360 at 113.475 kb on - strand, within H281DRAFT_05360 at 113.475 kb on - strand, within H281DRAFT_05360 at 113.562 kb on - strand, within H281DRAFT_05360 at 113.562 kb on - strand, within H281DRAFT_05360 at 113.604 kb on + strand, within H281DRAFT_05360 at 113.605 kb on - strand, within H281DRAFT_05360 at 113.646 kb on - strand, within H281DRAFT_05360 at 113.669 kb on + strand, within H281DRAFT_05360 at 113.669 kb on + strand, within H281DRAFT_05360 at 113.670 kb on - strand, within H281DRAFT_05360 at 113.670 kb on - strand, within H281DRAFT_05360 at 113.753 kb on + strand, within H281DRAFT_05360 at 113.754 kb on - strand, within H281DRAFT_05360 at 113.868 kb on - strand, within H281DRAFT_05360 at 113.870 kb on - strand, within H281DRAFT_05360 at 114.143 kb on + strand, within H281DRAFT_05360 at 114.143 kb on + strand, within H281DRAFT_05360 at 114.143 kb on + strand, within H281DRAFT_05360 at 114.179 kb on + strand, within H281DRAFT_05360 at 114.179 kb on + strand, within H281DRAFT_05360 at 114.179 kb on + strand, within H281DRAFT_05360 at 114.179 kb on + strand, within H281DRAFT_05360 at 114.237 kb on + strand, within H281DRAFT_05360 at 114.237 kb on + strand, within H281DRAFT_05360 at 114.238 kb on - strand, within H281DRAFT_05360 at 114.300 kb on - strand, within H281DRAFT_05360 at 114.388 kb on + strand at 114.388 kb on + strand at 114.389 kb on - strand at 114.399 kb on + strand at 114.399 kb on + strand at 114.399 kb on + strand at 114.400 kb on - strand at 114.401 kb on + strand at 114.402 kb on - strand at 114.411 kb on + strand at 114.534 kb on + strand at 114.534 kb on + strand at 114.534 kb on + strand at 114.534 kb on + strand at 114.535 kb on - strand at 114.535 kb on - strand at 114.535 kb on - strand at 114.535 kb on - strand at 114.565 kb on + strand at 114.565 kb on + strand at 114.566 kb on - strand at 114.566 kb on - strand at 114.566 kb on - strand at 114.566 kb on - strand at 114.571 kb on + strand at 114.591 kb on - strand at 114.645 kb on + strand at 114.646 kb on - strand at 114.646 kb on - strand at 114.655 kb on + strand at 114.655 kb on + strand at 114.655 kb on + strand at 114.656 kb on - strand at 114.656 kb on - strand at 114.742 kb on + strand, within H281DRAFT_05361 at 114.742 kb on + strand, within H281DRAFT_05361 at 114.742 kb on + strand, within H281DRAFT_05361 at 114.742 kb on + strand, within H281DRAFT_05361 at 114.742 kb on + strand, within H281DRAFT_05361 at 114.743 kb on - strand, within H281DRAFT_05361 at 114.743 kb on - strand, within H281DRAFT_05361 at 114.758 kb on + strand, within H281DRAFT_05361 at 114.864 kb on - strand, within H281DRAFT_05361 at 114.882 kb on + strand, within H281DRAFT_05361 at 114.882 kb on + strand at 114.882 kb on + strand, within H281DRAFT_05361 at 114.939 kb on - strand, within H281DRAFT_05361 at 114.939 kb on - strand, within H281DRAFT_05361 at 115.014 kb on + strand, within H281DRAFT_05361 at 115.014 kb on + strand, within H281DRAFT_05361 at 115.014 kb on + strand, within H281DRAFT_05361 at 115.014 kb on + strand, within H281DRAFT_05361 at 115.077 kb on + strand, within H281DRAFT_05361 at 115.079 kb on + strand, within H281DRAFT_05361 at 115.079 kb on + strand, within H281DRAFT_05361 at 115.080 kb on - strand, within H281DRAFT_05361 at 115.080 kb on - strand, within H281DRAFT_05361 at 115.080 kb on - strand, within H281DRAFT_05361 at 115.239 kb on - strand, within H281DRAFT_05361 at 115.272 kb on + strand at 115.272 kb on + strand at 115.273 kb on - strand at 115.273 kb on - strand at 115.273 kb on - strand at 115.273 kb on - strand at 115.273 kb on - strand at 115.303 kb on + strand at 115.304 kb on - strand at 115.304 kb on - strand at 115.304 kb on - strand at 115.322 kb on + strand at 115.346 kb on - strand at 115.346 kb on - strand at 115.366 kb on - strand at 115.392 kb on - strand at 115.403 kb on + strand at 115.403 kb on + strand at 115.403 kb on + strand at 115.404 kb on - strand at 115.404 kb on - strand at 115.404 kb on - strand at 115.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 112,241 + -0.8 112,242 - +1.5 112,242 - +0.6 112,323 - +0.0 112,323 - -1.5 112,381 + -0.2 112,381 + +0.2 112,494 + H281DRAFT_05359 0.12 -1.4 112,495 - H281DRAFT_05359 0.12 -0.1 112,495 - H281DRAFT_05359 0.12 -0.2 112,495 - H281DRAFT_05359 0.12 -0.4 112,674 + H281DRAFT_05359 0.35 +1.7 112,674 + H281DRAFT_05359 0.35 +0.2 112,704 + H281DRAFT_05359 0.39 -0.5 112,704 + H281DRAFT_05359 0.39 -0.0 112,704 + H281DRAFT_05359 0.39 +0.1 112,704 + H281DRAFT_05359 0.39 -0.7 112,705 - H281DRAFT_05359 0.39 +0.6 112,705 - H281DRAFT_05359 0.39 +0.6 112,705 - H281DRAFT_05359 0.39 +0.1 112,705 - H281DRAFT_05359 0.39 -0.9 112,705 - H281DRAFT_05359 0.39 -0.6 112,705 - H281DRAFT_05359 0.39 -0.6 112,705 - H281DRAFT_05359 0.39 +1.0 112,705 - H281DRAFT_05359 0.39 +0.3 112,795 - H281DRAFT_05359 0.51 -0.1 112,935 + H281DRAFT_05359 0.69 +0.8 112,936 - H281DRAFT_05359 0.69 -0.9 112,936 - H281DRAFT_05359 0.69 -0.6 113,077 - H281DRAFT_05359 0.87 +0.3 113,077 - H281DRAFT_05359 0.87 -0.6 113,106 + -0.0 113,107 - -1.0 113,172 + -0.1 113,231 + +0.1 113,231 + -0.0 113,231 + -0.0 113,231 + -0.1 113,231 + -4.0 113,232 - +0.4 113,232 - -0.5 113,232 - -0.2 113,232 - -0.1 113,267 + -0.2 113,268 - +0.3 113,297 + -0.8 113,298 - -0.5 113,345 + H281DRAFT_05360 0.13 +0.3 113,472 + H281DRAFT_05360 0.22 -0.5 113,473 - H281DRAFT_05360 0.22 -0.0 113,474 + H281DRAFT_05360 0.22 +2.8 113,474 + H281DRAFT_05360 0.22 +0.7 113,475 - H281DRAFT_05360 0.22 -0.4 113,475 - H281DRAFT_05360 0.22 +0.2 113,475 - H281DRAFT_05360 0.22 -1.2 113,475 - H281DRAFT_05360 0.22 -2.0 113,562 - H281DRAFT_05360 0.29 -0.1 113,562 - H281DRAFT_05360 0.29 +1.8 113,604 + H281DRAFT_05360 0.32 -0.1 113,605 - H281DRAFT_05360 0.32 +0.0 113,646 - H281DRAFT_05360 0.35 -2.4 113,669 + H281DRAFT_05360 0.37 +1.3 113,669 + H281DRAFT_05360 0.37 +0.5 113,670 - H281DRAFT_05360 0.37 -0.1 113,670 - H281DRAFT_05360 0.37 -0.6 113,753 + H281DRAFT_05360 0.43 +0.4 113,754 - H281DRAFT_05360 0.43 +0.2 113,868 - H281DRAFT_05360 0.51 -1.1 113,870 - H281DRAFT_05360 0.52 +0.1 114,143 + H281DRAFT_05360 0.72 +0.3 114,143 + H281DRAFT_05360 0.72 -1.7 114,143 + H281DRAFT_05360 0.72 -0.6 114,179 + H281DRAFT_05360 0.74 +0.1 114,179 + H281DRAFT_05360 0.74 +1.0 114,179 + H281DRAFT_05360 0.74 -0.2 114,179 + H281DRAFT_05360 0.74 -0.6 114,237 + H281DRAFT_05360 0.79 +0.9 114,237 + H281DRAFT_05360 0.79 -0.2 114,238 - H281DRAFT_05360 0.79 -0.5 114,300 - H281DRAFT_05360 0.83 +0.1 114,388 + +0.3 114,388 + +0.3 114,389 - +0.1 114,399 + -1.2 114,399 + +1.0 114,399 + +0.1 114,400 - +0.4 114,401 + -0.2 114,402 - -0.3 114,411 + +1.3 114,534 + -0.5 114,534 + -0.6 114,534 + -0.1 114,534 + -0.7 114,535 - -0.6 114,535 - -0.3 114,535 - -0.8 114,535 - -0.1 114,565 + -0.4 114,565 + -0.2 114,566 - +0.3 114,566 - -0.5 114,566 - -0.4 114,566 - -0.4 114,571 + -1.3 114,591 - -0.6 114,645 + +0.4 114,646 - +0.4 114,646 - -0.7 114,655 + -0.2 114,655 + -0.2 114,655 + +0.0 114,656 - -0.0 114,656 - +0.3 114,742 + H281DRAFT_05361 0.15 -0.5 114,742 + H281DRAFT_05361 0.15 -0.1 114,742 + H281DRAFT_05361 0.15 +0.1 114,742 + H281DRAFT_05361 0.15 +0.1 114,742 + H281DRAFT_05361 0.15 +0.1 114,743 - H281DRAFT_05361 0.15 +0.4 114,743 - H281DRAFT_05361 0.15 +0.2 114,758 + H281DRAFT_05361 0.17 +0.4 114,864 - H281DRAFT_05361 0.33 +0.3 114,882 + H281DRAFT_05361 0.36 +0.2 114,882 + +1.1 114,882 + H281DRAFT_05361 0.36 -0.4 114,939 - H281DRAFT_05361 0.44 +1.4 114,939 - H281DRAFT_05361 0.44 -0.1 115,014 + H281DRAFT_05361 0.56 +0.2 115,014 + H281DRAFT_05361 0.56 -1.2 115,014 + H281DRAFT_05361 0.56 -0.1 115,014 + H281DRAFT_05361 0.56 -0.9 115,077 + H281DRAFT_05361 0.65 +0.1 115,079 + H281DRAFT_05361 0.65 +0.2 115,079 + H281DRAFT_05361 0.65 -0.4 115,080 - H281DRAFT_05361 0.65 -0.8 115,080 - H281DRAFT_05361 0.65 -0.9 115,080 - H281DRAFT_05361 0.65 -0.2 115,239 - H281DRAFT_05361 0.89 -1.7 115,272 + +0.7 115,272 + -1.9 115,273 - -0.1 115,273 - +1.7 115,273 - +0.5 115,273 - +0.2 115,273 - +0.2 115,303 + +0.6 115,304 - -0.8 115,304 - -0.9 115,304 - +0.8 115,322 + +3.0 115,346 - +0.3 115,346 - +1.0 115,366 - +0.1 115,392 - -0.9 115,403 + +0.4 115,403 + +0.4 115,403 + -0.3 115,404 - +0.9 115,404 - -0.4 115,404 - -0.8 115,509 - +0.0
Or see this region's nucleotide sequence