Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04073

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04072 and H281DRAFT_04073 are separated by 241 nucleotidesH281DRAFT_04073 and H281DRAFT_04074 are separated by 79 nucleotides H281DRAFT_04072: H281DRAFT_04072 - stearoyl-CoA desaturase (delta-9 desaturase), at 173,249 to 174,406 _04072 H281DRAFT_04073: H281DRAFT_04073 - Small-conductance mechanosensitive channel, at 174,648 to 176,021 _04073 H281DRAFT_04074: H281DRAFT_04074 - 16S rRNA (cytosine967-C5)-methyltransferase, at 176,101 to 177,360 _04074 Position (kb) 174 175 176 177Strain fitness (log2 ratio) -2 -1 0 1 2 3at 174.514 kb on + strandat 174.620 kb on - strandat 174.650 kb on - strandat 174.653 kb on + strandat 174.653 kb on + strandat 174.653 kb on + strandat 174.654 kb on - strandat 174.726 kb on + strandat 174.874 kb on - strand, within H281DRAFT_04073at 174.874 kb on - strand, within H281DRAFT_04073at 174.906 kb on + strand, within H281DRAFT_04073at 174.907 kb on - strand, within H281DRAFT_04073at 175.003 kb on + strand, within H281DRAFT_04073at 175.004 kb on - strand, within H281DRAFT_04073at 175.031 kb on - strand, within H281DRAFT_04073at 175.045 kb on + strand, within H281DRAFT_04073at 175.046 kb on - strand, within H281DRAFT_04073at 175.130 kb on + strand, within H281DRAFT_04073at 175.130 kb on + strand, within H281DRAFT_04073at 175.131 kb on - strand, within H281DRAFT_04073at 175.131 kb on - strand, within H281DRAFT_04073at 175.131 kb on - strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.234 kb on + strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.235 kb on - strand, within H281DRAFT_04073at 175.238 kb on + strand, within H281DRAFT_04073at 175.238 kb on + strand, within H281DRAFT_04073at 175.239 kb on - strand, within H281DRAFT_04073at 175.239 kb on - strand, within H281DRAFT_04073at 175.700 kb on - strand, within H281DRAFT_04073at 175.700 kb on - strand, within H281DRAFT_04073at 176.091 kb on + strandat 176.091 kb on + strandat 176.172 kb on - strandat 176.306 kb on + strand, within H281DRAFT_04074at 176.606 kb on + strand, within H281DRAFT_04074at 176.606 kb on + strand, within H281DRAFT_04074at 176.607 kb on - strand, within H281DRAFT_04074at 176.607 kb on - strand, within H281DRAFT_04074at 176.607 kb on - strand, within H281DRAFT_04074at 176.866 kb on + strand, within H281DRAFT_04074at 176.866 kb on + strand, within H281DRAFT_04074at 176.866 kb on + strand, within H281DRAFT_04074at 176.866 kb on + strand, within H281DRAFT_04074at 176.867 kb on - strand, within H281DRAFT_04074at 176.921 kb on + strand, within H281DRAFT_04074at 176.984 kb on + strand, within H281DRAFT_04074at 176.985 kb on - strand, within H281DRAFT_04074at 176.985 kb on - strand, within H281DRAFT_04074at 176.985 kb on - strand, within H281DRAFT_04074at 176.985 kb on - strand, within H281DRAFT_04074

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
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174,514 + -1.2
174,620 - -1.1
174,650 - -0.4
174,653 + -0.8
174,653 + +0.2
174,653 + +1.0
174,654 - -0.0
174,726 + +1.3
174,874 - H281DRAFT_04073 0.16 -0.6
174,874 - H281DRAFT_04073 0.16 +0.3
174,906 + H281DRAFT_04073 0.19 +1.0
174,907 - H281DRAFT_04073 0.19 -0.6
175,003 + H281DRAFT_04073 0.26 -0.7
175,004 - H281DRAFT_04073 0.26 -0.4
175,031 - H281DRAFT_04073 0.28 -0.7
175,045 + H281DRAFT_04073 0.29 +1.0
175,046 - H281DRAFT_04073 0.29 +0.0
175,130 + H281DRAFT_04073 0.35 +0.5
175,130 + H281DRAFT_04073 0.35 -0.7
175,131 - H281DRAFT_04073 0.35 +0.6
175,131 - H281DRAFT_04073 0.35 +1.2
175,131 - H281DRAFT_04073 0.35 +0.1
175,234 + H281DRAFT_04073 0.43 +0.5
175,234 + H281DRAFT_04073 0.43 -0.4
175,234 + H281DRAFT_04073 0.43 -0.6
175,234 + H281DRAFT_04073 0.43 +1.2
175,234 + H281DRAFT_04073 0.43 +0.6
175,234 + H281DRAFT_04073 0.43 +0.2
175,234 + H281DRAFT_04073 0.43 -0.2
175,235 - H281DRAFT_04073 0.43 -0.3
175,235 - H281DRAFT_04073 0.43 +0.3
175,235 - H281DRAFT_04073 0.43 -0.6
175,235 - H281DRAFT_04073 0.43 +0.0
175,235 - H281DRAFT_04073 0.43 +0.5
175,235 - H281DRAFT_04073 0.43 -0.2
175,235 - H281DRAFT_04073 0.43 -0.4
175,235 - H281DRAFT_04073 0.43 +1.1
175,235 - H281DRAFT_04073 0.43 +1.6
175,235 - H281DRAFT_04073 0.43 -0.2
175,238 + H281DRAFT_04073 0.43 +0.7
175,238 + H281DRAFT_04073 0.43 -0.4
175,239 - H281DRAFT_04073 0.43 +0.4
175,239 - H281DRAFT_04073 0.43 +0.4
175,700 - H281DRAFT_04073 0.77 +0.4
175,700 - H281DRAFT_04073 0.77 +2.0
176,091 + -2.5
176,091 + -0.2
176,172 - +1.1
176,306 + H281DRAFT_04074 0.16 +1.0
176,606 + H281DRAFT_04074 0.40 -1.5
176,606 + H281DRAFT_04074 0.40 +3.6
176,607 - H281DRAFT_04074 0.40 +0.1
176,607 - H281DRAFT_04074 0.40 +0.5
176,607 - H281DRAFT_04074 0.40 -0.2
176,866 + H281DRAFT_04074 0.61 +0.4
176,866 + H281DRAFT_04074 0.61 +1.1
176,866 + H281DRAFT_04074 0.61 -0.2
176,866 + H281DRAFT_04074 0.61 +0.2
176,867 - H281DRAFT_04074 0.61 -0.1
176,921 + H281DRAFT_04074 0.65 -0.0
176,984 + H281DRAFT_04074 0.70 +0.2
176,985 - H281DRAFT_04074 0.70 -0.5
176,985 - H281DRAFT_04074 0.70 -0.0
176,985 - H281DRAFT_04074 0.70 -0.1
176,985 - H281DRAFT_04074 0.70 +0.2

Or see this region's nucleotide sequence