Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01371

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01370 and H281DRAFT_01371 are separated by 278 nucleotidesH281DRAFT_01371 and H281DRAFT_01372 are separated by 107 nucleotidesH281DRAFT_01372 and H281DRAFT_01373 overlap by 4 nucleotides H281DRAFT_01370: H281DRAFT_01370 - phospholipase C, at 214,203 to 215,672 _01370 H281DRAFT_01371: H281DRAFT_01371 - MFS transporter, AAHS family, 4-hydroxybenzoate transporter, at 215,951 to 217,294 _01371 H281DRAFT_01372: H281DRAFT_01372 - protocatechuate 4,5-dioxygenase alpha subunit (EC 1.13.11.8), at 217,402 to 217,761 _01372 H281DRAFT_01373: H281DRAFT_01373 - protocatechuate 4,5-dioxygenase beta subunit (EC 1.13.11.8), at 217,758 to 218,603 _01373 Position (kb) 215 216 217 218Strain fitness (log2 ratio) -1 0 1at 215.105 kb on - strand, within H281DRAFT_01370at 215.270 kb on - strand, within H281DRAFT_01370at 215.270 kb on - strand, within H281DRAFT_01370at 215.270 kb on - strand, within H281DRAFT_01370at 215.412 kb on + strand, within H281DRAFT_01370at 215.601 kb on + strandat 215.601 kb on + strandat 215.602 kb on - strandat 216.054 kb on - strandat 216.650 kb on - strand, within H281DRAFT_01371at 217.470 kb on - strand, within H281DRAFT_01372at 217.514 kb on - strand, within H281DRAFT_01372at 217.718 kb on + strand, within H281DRAFT_01372at 217.915 kb on + strand, within H281DRAFT_01373

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
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215,105 - H281DRAFT_01370 0.61 +1.1
215,270 - H281DRAFT_01370 0.73 +0.4
215,270 - H281DRAFT_01370 0.73 +1.1
215,270 - H281DRAFT_01370 0.73 -0.0
215,412 + H281DRAFT_01370 0.82 -1.3
215,601 + -0.6
215,601 + -0.1
215,602 - +0.5
216,054 - +1.1
216,650 - H281DRAFT_01371 0.52 -1.1
217,470 - H281DRAFT_01372 0.19 +0.6
217,514 - H281DRAFT_01372 0.31 -0.4
217,718 + H281DRAFT_01372 0.88 -0.1
217,915 + H281DRAFT_01373 0.19 -0.6

Or see this region's nucleotide sequence