Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01211

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01209 and H281DRAFT_01210 are separated by 30 nucleotidesH281DRAFT_01210 and H281DRAFT_01211 are separated by 397 nucleotidesH281DRAFT_01211 and H281DRAFT_01212 are separated by 244 nucleotidesH281DRAFT_01212 and H281DRAFT_01213 are separated by 49 nucleotidesH281DRAFT_01213 and H281DRAFT_01214 are separated by 29 nucleotides H281DRAFT_01209: H281DRAFT_01209 - putative MFS transporter, AGZA family, xanthine/uracil permease, at 48,541 to 49,842 _01209 H281DRAFT_01210: H281DRAFT_01210 - Protein of unknown function (DUF1488), at 49,873 to 50,163 _01210 H281DRAFT_01211: H281DRAFT_01211 - Transcriptional regulator of acetoin/glycerol metabolism, at 50,561 to 52,537 _01211 H281DRAFT_01212: H281DRAFT_01212 - Ferredoxin, at 52,782 to 53,093 _01212 H281DRAFT_01213: H281DRAFT_01213 - Protein of unknown function (DUF1653), at 53,143 to 53,346 _01213 H281DRAFT_01214: H281DRAFT_01214 - Predicted transcriptional regulator YheO, contains PAS and DNA-binding HTH domains, at 53,376 to 54,020 _01214 Position (kb) 50 51 52 53Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 49.594 kb on + strand, within H281DRAFT_01209at 49.697 kb on - strand, within H281DRAFT_01209at 49.735 kb on + strandat 49.735 kb on + strandat 49.735 kb on + strandat 49.826 kb on - strandat 49.826 kb on - strandat 49.826 kb on - strandat 49.829 kb on + strandat 49.830 kb on - strandat 49.830 kb on - strandat 49.896 kb on - strandat 49.907 kb on + strand, within H281DRAFT_01210at 50.129 kb on + strand, within H281DRAFT_01210at 50.130 kb on - strand, within H281DRAFT_01210at 50.261 kb on - strandat 50.261 kb on - strandat 50.470 kb on - strandat 50.470 kb on - strandat 50.531 kb on - strandat 50.726 kb on + strandat 50.728 kb on + strandat 50.989 kb on + strand, within H281DRAFT_01211at 51.235 kb on + strand, within H281DRAFT_01211at 51.441 kb on - strand, within H281DRAFT_01211at 51.441 kb on - strand, within H281DRAFT_01211at 51.793 kb on + strand, within H281DRAFT_01211at 52.029 kb on - strand, within H281DRAFT_01211at 52.029 kb on - strand, within H281DRAFT_01211at 52.218 kb on + strand, within H281DRAFT_01211at 52.218 kb on + strand, within H281DRAFT_01211at 52.219 kb on - strand, within H281DRAFT_01211at 52.219 kb on - strand, within H281DRAFT_01211at 52.373 kb on - strandat 52.535 kb on + strandat 52.536 kb on - strandat 52.536 kb on - strandat 52.558 kb on + strandat 52.558 kb on + strandat 52.563 kb on + strandat 52.660 kb on + strandat 52.661 kb on - strandat 52.718 kb on - strandat 52.916 kb on + strand, within H281DRAFT_01212at 52.916 kb on + strand, within H281DRAFT_01212at 52.916 kb on + strand, within H281DRAFT_01212at 52.917 kb on - strand, within H281DRAFT_01212at 52.917 kb on - strand, within H281DRAFT_01212at 52.964 kb on + strand, within H281DRAFT_01212at 52.964 kb on + strand, within H281DRAFT_01212at 52.965 kb on - strand, within H281DRAFT_01212at 53.010 kb on + strand, within H281DRAFT_01212at 53.010 kb on + strand, within H281DRAFT_01212at 53.011 kb on - strand, within H281DRAFT_01212at 53.011 kb on - strandat 53.092 kb on - strandat 53.304 kb on - strand, within H281DRAFT_01213at 53.307 kb on + strand, within H281DRAFT_01213at 53.308 kb on - strand, within H281DRAFT_01213at 53.318 kb on + strand, within H281DRAFT_01213at 53.319 kb on - strand, within H281DRAFT_01213at 53.319 kb on - strand, within H281DRAFT_01213at 53.409 kb on - strandat 53.410 kb on + strandat 53.513 kb on + strand, within H281DRAFT_01214at 53.513 kb on + strand, within H281DRAFT_01214at 53.514 kb on - strand, within H281DRAFT_01214at 53.514 kb on - strand, within H281DRAFT_01214at 53.514 kb on - strand, within H281DRAFT_01214

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
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49,594 + H281DRAFT_01209 0.81 -1.8
49,697 - H281DRAFT_01209 0.89 -0.9
49,735 + -0.3
49,735 + -0.8
49,735 + +0.8
49,826 - +1.1
49,826 - +0.1
49,826 - -0.8
49,829 + -0.6
49,830 - +0.1
49,830 - +0.6
49,896 - -0.9
49,907 + H281DRAFT_01210 0.12 +1.0
50,129 + H281DRAFT_01210 0.88 -0.3
50,130 - H281DRAFT_01210 0.88 -0.4
50,261 - -0.9
50,261 - +0.5
50,470 - +0.3
50,470 - +1.2
50,531 - +0.8
50,726 + -2.6
50,728 + -1.7
50,989 + H281DRAFT_01211 0.22 -0.5
51,235 + H281DRAFT_01211 0.34 +0.3
51,441 - H281DRAFT_01211 0.45 +0.8
51,441 - H281DRAFT_01211 0.45 -0.9
51,793 + H281DRAFT_01211 0.62 -1.9
52,029 - H281DRAFT_01211 0.74 +0.2
52,029 - H281DRAFT_01211 0.74 -2.7
52,218 + H281DRAFT_01211 0.84 +1.3
52,218 + H281DRAFT_01211 0.84 -0.9
52,219 - H281DRAFT_01211 0.84 +3.8
52,219 - H281DRAFT_01211 0.84 +0.1
52,373 - -2.3
52,535 + -0.3
52,536 - -3.2
52,536 - -0.4
52,558 + +1.0
52,558 + -0.3
52,563 + +0.2
52,660 + -0.7
52,661 - +0.7
52,718 - +1.1
52,916 + H281DRAFT_01212 0.43 +0.5
52,916 + H281DRAFT_01212 0.43 +0.6
52,916 + H281DRAFT_01212 0.43 +0.8
52,917 - H281DRAFT_01212 0.43 -0.1
52,917 - H281DRAFT_01212 0.43 +1.1
52,964 + H281DRAFT_01212 0.58 +0.3
52,964 + H281DRAFT_01212 0.58 +0.6
52,965 - H281DRAFT_01212 0.59 +0.5
53,010 + H281DRAFT_01212 0.73 +0.9
53,010 + H281DRAFT_01212 0.73 -0.5
53,011 - H281DRAFT_01212 0.73 +0.2
53,011 - -1.6
53,092 - +0.7
53,304 - H281DRAFT_01213 0.79 -0.1
53,307 + H281DRAFT_01213 0.80 -1.4
53,308 - H281DRAFT_01213 0.81 -0.6
53,318 + H281DRAFT_01213 0.86 -0.1
53,319 - H281DRAFT_01213 0.86 +0.2
53,319 - H281DRAFT_01213 0.86 -0.4
53,409 - +2.0
53,410 + +0.2
53,513 + H281DRAFT_01214 0.21 +0.5
53,513 + H281DRAFT_01214 0.21 +1.8
53,514 - H281DRAFT_01214 0.21 -0.0
53,514 - H281DRAFT_01214 0.21 +0.1
53,514 - H281DRAFT_01214 0.21 +0.6

Or see this region's nucleotide sequence