Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00018 and H281DRAFT_00019 are separated by 670 nucleotides H281DRAFT_00019 and H281DRAFT_00020 are separated by 165 nucleotides
H281DRAFT_00018: H281DRAFT_00018 - hypothetical protein, at 19,259 to 19,903
_00018
H281DRAFT_00019: H281DRAFT_00019 - transcriptional regulator, LysR family, at 20,574 to 21,485
_00019
H281DRAFT_00020: H281DRAFT_00020 - Glutathione S-transferase, at 21,651 to 22,349
_00020
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1
2
3 at 19.599 kb on + strand, within H281DRAFT_00018 at 19.635 kb on + strand, within H281DRAFT_00018 at 19.637 kb on + strand, within H281DRAFT_00018 at 19.638 kb on - strand, within H281DRAFT_00018 at 19.680 kb on - strand, within H281DRAFT_00018 at 19.684 kb on + strand, within H281DRAFT_00018 at 19.684 kb on + strand, within H281DRAFT_00018 at 19.685 kb on - strand, within H281DRAFT_00018 at 19.685 kb on - strand, within H281DRAFT_00018 at 19.685 kb on - strand, within H281DRAFT_00018 at 19.685 kb on - strand, within H281DRAFT_00018 at 19.755 kb on + strand, within H281DRAFT_00018 at 19.756 kb on - strand, within H281DRAFT_00018 at 19.756 kb on - strand, within H281DRAFT_00018 at 19.793 kb on - strand at 19.836 kb on + strand, within H281DRAFT_00018 at 20.050 kb on - strand at 20.050 kb on - strand at 20.050 kb on - strand at 20.074 kb on + strand at 20.180 kb on + strand at 20.181 kb on - strand at 20.181 kb on - strand at 20.366 kb on + strand at 20.418 kb on + strand at 20.418 kb on + strand at 20.419 kb on - strand at 20.419 kb on - strand at 20.419 kb on - strand at 20.424 kb on - strand at 20.576 kb on - strand at 20.595 kb on + strand at 20.596 kb on - strand at 20.628 kb on + strand at 20.740 kb on + strand, within H281DRAFT_00019 at 20.803 kb on - strand, within H281DRAFT_00019 at 20.885 kb on + strand, within H281DRAFT_00019 at 20.896 kb on + strand, within H281DRAFT_00019 at 20.896 kb on + strand, within H281DRAFT_00019 at 20.896 kb on + strand, within H281DRAFT_00019 at 20.897 kb on - strand, within H281DRAFT_00019 at 21.002 kb on + strand, within H281DRAFT_00019 at 21.002 kb on + strand, within H281DRAFT_00019 at 21.003 kb on - strand, within H281DRAFT_00019 at 21.003 kb on - strand, within H281DRAFT_00019 at 21.004 kb on + strand, within H281DRAFT_00019 at 21.004 kb on + strand, within H281DRAFT_00019 at 21.036 kb on + strand, within H281DRAFT_00019 at 21.093 kb on - strand, within H281DRAFT_00019 at 21.093 kb on - strand, within H281DRAFT_00019 at 21.093 kb on - strand, within H281DRAFT_00019 at 21.096 kb on + strand, within H281DRAFT_00019 at 21.097 kb on - strand, within H281DRAFT_00019 at 21.549 kb on - strand at 21.606 kb on + strand at 21.606 kb on + strand at 21.763 kb on - strand, within H281DRAFT_00020 at 21.889 kb on - strand, within H281DRAFT_00020 at 22.023 kb on - strand, within H281DRAFT_00020 at 22.038 kb on - strand, within H281DRAFT_00020 at 22.178 kb on + strand, within H281DRAFT_00020 at 22.178 kb on + strand, within H281DRAFT_00020 at 22.179 kb on - strand, within H281DRAFT_00020 at 22.302 kb on + strand at 22.407 kb on + strand at 22.436 kb on + strand at 22.441 kb on + strand at 22.441 kb on + strand at 22.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 19,599 + H281DRAFT_00018 0.53 +0.3 19,635 + H281DRAFT_00018 0.58 +0.3 19,637 + H281DRAFT_00018 0.59 -0.4 19,638 - H281DRAFT_00018 0.59 -1.2 19,680 - H281DRAFT_00018 0.65 -0.2 19,684 + H281DRAFT_00018 0.66 -0.1 19,684 + H281DRAFT_00018 0.66 -0.3 19,685 - H281DRAFT_00018 0.66 +0.3 19,685 - H281DRAFT_00018 0.66 -0.2 19,685 - H281DRAFT_00018 0.66 +0.3 19,685 - H281DRAFT_00018 0.66 -0.0 19,755 + H281DRAFT_00018 0.77 -0.2 19,756 - H281DRAFT_00018 0.77 -1.7 19,756 - H281DRAFT_00018 0.77 -0.2 19,793 - +1.8 19,836 + H281DRAFT_00018 0.89 +0.7 20,050 - -0.1 20,050 - +0.5 20,050 - -0.4 20,074 + -0.3 20,180 + +1.1 20,181 - -0.4 20,181 - -0.6 20,366 + +0.5 20,418 + +0.3 20,418 + -0.8 20,419 - -0.8 20,419 - +0.2 20,419 - -0.3 20,424 - +0.8 20,576 - -1.7 20,595 + -0.4 20,596 - -0.4 20,628 + -0.5 20,740 + H281DRAFT_00019 0.18 -0.3 20,803 - H281DRAFT_00019 0.25 -0.1 20,885 + H281DRAFT_00019 0.34 +0.6 20,896 + H281DRAFT_00019 0.35 -1.5 20,896 + H281DRAFT_00019 0.35 +0.0 20,896 + H281DRAFT_00019 0.35 +0.3 20,897 - H281DRAFT_00019 0.35 +0.3 21,002 + H281DRAFT_00019 0.47 -0.8 21,002 + H281DRAFT_00019 0.47 -0.5 21,003 - H281DRAFT_00019 0.47 -0.5 21,003 - H281DRAFT_00019 0.47 +1.0 21,004 + H281DRAFT_00019 0.47 -0.4 21,004 + H281DRAFT_00019 0.47 -1.0 21,036 + H281DRAFT_00019 0.51 -1.5 21,093 - H281DRAFT_00019 0.57 +0.4 21,093 - H281DRAFT_00019 0.57 +0.3 21,093 - H281DRAFT_00019 0.57 -0.1 21,096 + H281DRAFT_00019 0.57 -0.8 21,097 - H281DRAFT_00019 0.57 -1.3 21,549 - -0.4 21,606 + +2.1 21,606 + +0.3 21,763 - H281DRAFT_00020 0.16 -0.6 21,889 - H281DRAFT_00020 0.34 -0.9 22,023 - H281DRAFT_00020 0.53 +0.8 22,038 - H281DRAFT_00020 0.55 +0.8 22,178 + H281DRAFT_00020 0.75 -0.2 22,178 + H281DRAFT_00020 0.75 -0.1 22,179 - H281DRAFT_00020 0.76 +0.4 22,302 + +0.0 22,407 + +1.8 22,436 + +0.5 22,441 + -0.2 22,441 + +2.8 22,457 - +0.8
Or see this region's nucleotide sequence