Experiment: MoYLS4 with Dimethyl Sulfoxide 5 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisH and DvMF_2306 are separated by 712 nucleotides DvMF_2306 and DvMF_2307 are separated by 182 nucleotides
DvMF_2305: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) (from data) , at 2,895,267 to 2,895,905
hisH
DvMF_2306: DvMF_2306 - carbamoyl-phosphate synthase, large subunit (RefSeq), at 2,896,618 to 2,899,854
_2306
DvMF_2307: DvMF_2307 - amidophosphoribosyltransferase (RefSeq), at 2,900,037 to 2,901,440
_2307
Position (kb)
2896
2897
2898
2899
2900 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2895.738 kb on - strand, within hisH at 2895.840 kb on - strand, within hisH at 2895.896 kb on - strand at 2895.958 kb on + strand at 2896.166 kb on + strand at 2896.658 kb on + strand at 2896.750 kb on + strand at 2896.750 kb on + strand at 2896.858 kb on + strand at 2896.883 kb on - strand at 2896.900 kb on + strand at 2896.912 kb on + strand at 2896.960 kb on - strand, within DvMF_2306 at 2896.993 kb on + strand, within DvMF_2306 at 2897.480 kb on - strand, within DvMF_2306 at 2897.654 kb on + strand, within DvMF_2306 at 2897.764 kb on + strand, within DvMF_2306 at 2897.806 kb on + strand, within DvMF_2306 at 2898.178 kb on - strand, within DvMF_2306 at 2898.217 kb on + strand, within DvMF_2306 at 2898.872 kb on + strand, within DvMF_2306 at 2899.087 kb on - strand, within DvMF_2306 at 2899.104 kb on + strand, within DvMF_2306 at 2899.352 kb on + strand, within DvMF_2306 at 2899.559 kb on + strand at 2899.687 kb on - strand at 2899.793 kb on - strand at 2899.819 kb on - strand at 2899.824 kb on - strand at 2899.895 kb on - strand at 2900.172 kb on - strand at 2900.382 kb on - strand, within DvMF_2307 at 2900.521 kb on - strand, within DvMF_2307 at 2900.725 kb on + strand, within DvMF_2307 at 2900.726 kb on - strand, within DvMF_2307 at 2900.803 kb on + strand, within DvMF_2307
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 5 % remove 2,895,738 - hisH DvMF_2305 0.74 -1.9 2,895,840 - hisH DvMF_2305 0.90 -1.7 2,895,896 - -2.1 2,895,958 + -0.4 2,896,166 + -0.4 2,896,658 + -2.5 2,896,750 + -2.1 2,896,750 + -3.9 2,896,858 + -4.0 2,896,883 - -2.1 2,896,900 + -3.6 2,896,912 + -2.8 2,896,960 - DvMF_2306 0.11 -2.2 2,896,993 + DvMF_2306 0.12 -3.3 2,897,480 - DvMF_2306 0.27 -3.0 2,897,654 + DvMF_2306 0.32 -3.5 2,897,764 + DvMF_2306 0.35 -3.3 2,897,806 + DvMF_2306 0.37 -1.9 2,898,178 - DvMF_2306 0.48 -3.0 2,898,217 + DvMF_2306 0.49 -3.4 2,898,872 + DvMF_2306 0.70 -3.4 2,899,087 - DvMF_2306 0.76 -3.8 2,899,104 + DvMF_2306 0.77 -3.2 2,899,352 + DvMF_2306 0.84 -2.2 2,899,559 + -0.7 2,899,687 - -4.4 2,899,793 - -1.3 2,899,819 - -0.2 2,899,824 - -0.7 2,899,895 - -0.6 2,900,172 - -3.3 2,900,382 - DvMF_2307 0.25 -3.7 2,900,521 - DvMF_2307 0.34 -4.5 2,900,725 + DvMF_2307 0.49 -2.8 2,900,726 - DvMF_2307 0.49 -4.1 2,900,803 + DvMF_2307 0.55 -3.7
Or see this region's nucleotide sequence