Experiment: MoYLS4 with Dimethyl Sulfoxide 5 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1943 and DvMF_1944 are separated by 129 nucleotides DvMF_1944 and DvMF_1945 are separated by 87 nucleotides DvMF_1945 and DvMF_1946 are separated by 247 nucleotides
DvMF_1943: DvMF_1943 - ribonuclease II (RefSeq), at 2,398,885 to 2,401,092
_1943
DvMF_1944: DvMF_1944 - protein of unknown function DUF205 (RefSeq), at 2,401,222 to 2,401,950
_1944
DvMF_1945: DvMF_1945 - threonine synthase (EC 4.2.3.1) (from data) , at 2,402,038 to 2,403,516
_1945
DvMF_1946: DvMF_1946 - Phosphoribosylaminoimidazolecarboxamide formyltransferase (RefSeq), at 2,403,764 to 2,404,363
_1946
Position (kb)
2402
2403
2404 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2402.018 kb on + strand at 2402.176 kb on + strand at 2402.353 kb on - strand, within DvMF_1945 at 2402.809 kb on - strand, within DvMF_1945 at 2403.039 kb on + strand, within DvMF_1945 at 2403.047 kb on - strand, within DvMF_1945 at 2403.047 kb on - strand, within DvMF_1945 at 2403.404 kb on + strand at 2403.701 kb on - strand at 2403.902 kb on + strand, within DvMF_1946 at 2403.917 kb on + strand, within DvMF_1946 at 2404.014 kb on + strand, within DvMF_1946 at 2404.026 kb on + strand, within DvMF_1946 at 2404.071 kb on + strand, within DvMF_1946 at 2404.074 kb on - strand, within DvMF_1946 at 2404.212 kb on - strand, within DvMF_1946 at 2404.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 5 % remove 2,402,018 + -0.2 2,402,176 + -1.8 2,402,353 - DvMF_1945 0.21 -1.3 2,402,809 - DvMF_1945 0.52 -3.9 2,403,039 + DvMF_1945 0.68 -3.3 2,403,047 - DvMF_1945 0.68 -2.7 2,403,047 - DvMF_1945 0.68 -3.1 2,403,404 + -2.5 2,403,701 - +0.5 2,403,902 + DvMF_1946 0.23 -1.0 2,403,917 + DvMF_1946 0.26 -4.0 2,404,014 + DvMF_1946 0.42 -3.9 2,404,026 + DvMF_1946 0.44 -4.3 2,404,071 + DvMF_1946 0.51 -3.6 2,404,074 - DvMF_1946 0.52 -4.5 2,404,212 - DvMF_1946 0.75 -2.4 2,404,369 + -0.3
Or see this region's nucleotide sequence