Experiment: MoYLS4 with Dimethyl Sulfoxide 5 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1741 and DvMF_1742 are separated by 39 nucleotides DvMF_1742 and DvMF_1743 overlap by 4 nucleotides DvMF_1743 and DvMF_1744 overlap by 8 nucleotides
DvMF_1741: DvMF_1741 - tryptophan synthase subunit beta (RefSeq), at 2,128,334 to 2,129,518
_1741
DvMF_1742: DvMF_1742 - Phosphoribosylanthranilate isomerase (RefSeq), at 2,129,558 to 2,130,235
_1742
DvMF_1743: DvMF_1743 - Indole-3-glycerol-phosphate synthase (RefSeq), at 2,130,232 to 2,131,197
_1743
DvMF_1744: DvMF_1744 - anthranilate phosphoribosyltransferase (RefSeq), at 2,131,190 to 2,132,206
_1744
Position (kb)
2130
2131
2132 Strain fitness (log2 ratio)
-2
-1
0
1 at 2129.248 kb on + strand, within DvMF_1741 at 2129.251 kb on - strand, within DvMF_1741 at 2129.335 kb on + strand, within DvMF_1741 at 2129.368 kb on + strand, within DvMF_1741 at 2129.528 kb on - strand at 2129.646 kb on + strand, within DvMF_1742 at 2129.666 kb on + strand, within DvMF_1742 at 2129.865 kb on + strand, within DvMF_1742 at 2130.138 kb on + strand, within DvMF_1742 at 2130.178 kb on + strand at 2130.405 kb on - strand, within DvMF_1743 at 2130.452 kb on - strand, within DvMF_1743 at 2130.675 kb on + strand, within DvMF_1743 at 2130.840 kb on + strand, within DvMF_1743 at 2130.892 kb on + strand, within DvMF_1743 at 2130.909 kb on + strand, within DvMF_1743 at 2131.049 kb on + strand, within DvMF_1743 at 2131.261 kb on + strand at 2131.283 kb on + strand at 2131.283 kb on + strand at 2131.291 kb on - strand at 2131.338 kb on + strand, within DvMF_1744 at 2131.496 kb on - strand, within DvMF_1744 at 2131.611 kb on - strand, within DvMF_1744 at 2131.828 kb on - strand, within DvMF_1744 at 2131.897 kb on - strand, within DvMF_1744
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 5 % remove 2,129,248 + DvMF_1741 0.77 -1.6 2,129,251 - DvMF_1741 0.77 -1.5 2,129,335 + DvMF_1741 0.84 -1.5 2,129,368 + DvMF_1741 0.87 -2.0 2,129,528 - -0.1 2,129,646 + DvMF_1742 0.13 -1.0 2,129,666 + DvMF_1742 0.16 -1.2 2,129,865 + DvMF_1742 0.45 -1.2 2,130,138 + DvMF_1742 0.86 -1.0 2,130,178 + +0.3 2,130,405 - DvMF_1743 0.18 -0.6 2,130,452 - DvMF_1743 0.23 -0.4 2,130,675 + DvMF_1743 0.46 -1.3 2,130,840 + DvMF_1743 0.63 -1.1 2,130,892 + DvMF_1743 0.68 -2.1 2,130,909 + DvMF_1743 0.70 -0.6 2,131,049 + DvMF_1743 0.85 -1.3 2,131,261 + -1.8 2,131,283 + -1.7 2,131,283 + -1.6 2,131,291 - -1.3 2,131,338 + DvMF_1744 0.15 -1.5 2,131,496 - DvMF_1744 0.30 -1.1 2,131,611 - DvMF_1744 0.41 -1.5 2,131,828 - DvMF_1744 0.63 -1.2 2,131,897 - DvMF_1744 0.70 -1.2
Or see this region's nucleotide sequence