Experiment: MoYLS4 with Dimethyl Sulfoxide 5 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0007 and DvMF_R0002 are separated by 71 nucleotides DvMF_R0002 and DvMF_R0003 are separated by 57 nucleotides DvMF_R0003 and DvMF_R0004 are separated by 8 nucleotides DvMF_R0004 and DvMF_R0005 are separated by 13 nucleotides DvMF_R0005 and DvMF_0008 are separated by 475 nucleotides DvMF_0008 and DvMF_0009 are separated by 205 nucleotides
DvMF_0007: DvMF_0007 - protein of unknown function DUF302 (RefSeq), at 9,037 to 9,444
_0007
DvMF_R0002: DvMF_R0002 - tRNA-Gly (RefSeq), at 9,516 to 9,590
_R0002
DvMF_R0003: DvMF_R0003 - tRNA-Gly (RefSeq), at 9,648 to 9,722
_R0003
DvMF_R0004: DvMF_R0004 - tRNA-Cys (RefSeq), at 9,731 to 9,805
_R0004
DvMF_R0005: DvMF_R0005 - tRNA-Gly (RefSeq), at 9,819 to 9,893
_R0005
DvMF_0008: DvMF_0008 - transcriptional regulator, TraR/DksA family (RefSeq), at 10,369 to 10,731
_0008
DvMF_0009: DvMF_0009 - protein of unknown function DUF814 (RefSeq), at 10,937 to 12,919
_0009
Position (kb) 10
11 Strain fitness (log2 ratio) -1
0
1 at 9.399 kb on - strand, within DvMF_0007 at 9.412 kb on + strand at 9.553 kb on - strand, within DvMF_R0002 at 9.702 kb on + strand, within DvMF_R0003 at 10.109 kb on + strand at 10.951 kb on + strand at 11.107 kb on + strand at 11.337 kb on + strand, within DvMF_0009 at 11.371 kb on - strand, within DvMF_0009 at 11.413 kb on - strand, within DvMF_0009
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 5 % remove 9,399 - DvMF_0007 0.89 -0.3 9,412 + -0.7 9,553 - DvMF_R0002 0.49 -0.4 9,702 + DvMF_R0003 0.72 -0.2 10,109 + -0.7 10,951 + -0.2 11,107 + -0.2 11,337 + DvMF_0009 0.20 -0.4 11,371 - DvMF_0009 0.22 -0.1 11,413 - DvMF_0009 0.24 +0.0
Or see this region's nucleotide sequence